公开(公告)号：US20090275023A1

公开(公告)日：2009-11-05

申请号：US12156029

申请日：2008-05-29

申请人： Melanie Feola ,  Martin Adelson ,  Eli Mordechai ,  Lisa Novak

发明人： Melanie Feola ,  Martin Adelson ,  Eli Mordechai ,  Lisa Novak

IPC分类号： C12Q1/68 ,  C07H21/04

CPC分类号： C12Q1/689 ,  Y02A50/57

摘要： Disclosed are oligonucleotides useful in methods for determining whether a sample contains Borrelia afzelii, a spirochete which is a causative agent of Lyme disease in humans. These oligonucleotides, which have nucleotide sequences derived from a coding segment of the gene encoding the p24 gene for the outer surface protein of Borrelia afzelii, are useful as forward and reverse primers for a polymerase chain reaction using nucleic acids from a biological sample as templates, and as probes for detecting any resultant amplicon. Detection of an amplicon indicates the sample contains Borrelia afzelii. Real-time PCR and detection using florescence resonance energy transfer is disclosed.

摘要翻译： 公开了用于确定样品是否含有人类的莱姆病的致病因子的螺旋体（Borrelia afzelii）的方法中的寡核苷酸。 这些寡核苷酸具有衍生自编码b24基因的基因的编码区段的核苷酸序列，用于使用来自生物样品的核酸作为模板的聚合酶链反应的正向和反向引物， 以及用于检测任何所得扩增子的探针。 扩增子的检测表明样品含有Borrelia afzelii。 公开了使用荧光共振能量转移的实时PCR和检测。

公开(公告)号：US20060127934A1

公开(公告)日：2006-06-15

申请号：US11299362

申请日：2005-12-09

申请人： Jason Trama ,  Martin Adelson ,  Eli Mordechai

发明人： Jason Trama ,  Martin Adelson ,  Eli Mordechai

IPC分类号： C12Q1/68 ,  C07H21/04

CPC分类号： C12Q1/6895 ,  C12Q1/689 ,  C12Q2561/113 ,  C12Q2565/301 ,  C12Q2600/156

摘要： Oligonucleotides and methods for using these oligonucleotides in the detection of Aspergillus fumigatus are disclosed. Aspergillus fumigatus is the causative agent for medical conditions including invasive aspergillosis. The oligonucleotides of the invention have nucleotide sequences derived from the gene encoding the cytochrome P450 14 alpha-sterol demethylase (i.e., the Cyp51A protein) of Aspergillus fumigatus. The oligonucleotides of the invention include forward primers and reverse primers which in combination are capable of priming the synthesis of amplicons specific to cyp51A in polymerase chain reactions using nucleic acids isolated from Aspergillus fumigatus as templates. The oligonucleotides of the invention also include probes capable of detecting these cyp51A-specific amplicons. Thus, a biological sample is tested for the presence of Aspergillus fumigatus by isolating nucleic acid from the sample, conducting a polymerase chain reaction in a mixture containing this nucleic acid and these forward and reverse primers, and then determining, using an oligonucleotide probe, whether an amplicon is produced in the mixture, wherein detection of the amplicon indicates the presence of Aspergillus fumigatus in the sample. The oligonucleotides of the invention also include primers for nucleotide sequencing reactions to determine whether an isolate of Aspergillus fumigatus is more tolerant than wild-type Aspergillus fumigatus to a triazole, which is a compound commonly used as an antifungal drug. Specifically, a strand of a cyp51A-specific amplicon is at least partially sequenced using a nucleotide sequencing primer in a primer extension reaction. Identification of mutations giving rise to amino acid substitutions at positions 54, 138, 220, and 448 of the amino acid sequence of the wild-type Cyp51A protein indicates that the isolate of Aspergillus fumigatus from which the amplicon is derived exhibits decreased susceptibility to at least one triazole.

公开(公告)号：US20060088865A1

公开(公告)日：2006-04-27

申请号：US11246976

申请日：2005-10-07

申请人： Martin Adelson ,  Melanie Feola ,  Jason Trama ,  Eli Mordechai

发明人： Martin Adelson ,  Melanie Feola ,  Jason Trama ,  Eli Mordechai

IPC分类号： C12Q1/70 ,  C12Q1/68

CPC分类号： C12Q1/705

摘要： Methods are described herein for detecting and identifying distinct species of nucleic acids, in a single container, for example, from a certain genus of infectious agents or otherwise causative agents comprising, for example, providing a forward PCR primer common to a homologous gene region between the distinct species, and providing a reverse PCR primer common to a homologous gene region between the distinct species, to thereby define a PCR target region amongst the species, and providing a first oligonucleotide probe specific to a nucleic acid sequence within the target region that is characteristic of a first species, providing a second oligonucleotide probe specific to a nucleic acid sequence within the target region that is characteristic of a second species, wherein the first and second oligonucleotide probes are each detectably labeled with distinctly different detectable labels, conducting a PCR reaction in the container by means of the primers to amplify the target region amongst the species, and detecting the distinct labels, thereby identifying distinct species of nucleic acids corresponding to distinct species of infectious agents. Methods are preferred, for example, wherein the infectious agent is a member of the Herpesviridae family.

公开(公告)号：US20120015362A1

公开(公告)日：2012-01-19

申请号：US13135022

申请日：2011-06-23

申请人： Melanie Feola ,  Martin Adelson ,  Eli Mordechai ,  John Entwistle

发明人： Melanie Feola ,  Martin Adelson ,  Eli Mordechai ,  John Entwistle

IPC分类号： C12Q1/68 ,  C07H21/04

CPC分类号： C12Q1/6895 ,  C07H21/04 ,  C12Q2600/158

摘要： Disclosed are oligonucleotides useful in methods for determining whether a sample contains Cryptococcus neoformans, a causative agent for human cryptococcosis. These oligonucleotides, which have nucleotide sequences derived from a coding segment of the gene encoding the fungal specific transcription factor gene in Cryptococcus neoformans, are useful as forward and reverse primers for a polymerase chain reaction using nucleic acids from a biological sample as templates, and as probes for detecting any resultant amplicon. Detection of an amplicon indicates the sample contains Cryptococcus neoformans. Real-time PCR and detection using florescence resonance energy transfer is disclosed.

摘要翻译： 公开了用于确定样品是否含有新隐球菌隐球菌（人类隐球菌病的致病因子）的方法中的寡核苷酸。 这些寡核苷酸具有源自编码新型隐球菌中的真菌特异性转录因子基因的基因的编码区的核苷酸序列，可用作使用来自生物样品的核酸作为模板的聚合酶链反应的正向和反向引物，以及 用于检测任何所得扩增子的探针。 扩增子的检测表明样品含有新型隐球酵母。 公开了使用荧光共振能量转移的实时PCR和检测。

公开(公告)号：US20090246767A1

公开(公告)日：2009-10-01

申请号：US12214317

申请日：2008-06-18

申请人： Melanie Feola ,  Martin Adelson ,  Eli Mordechai ,  John Entwistle

发明人： Melanie Feola ,  Martin Adelson ,  Eli Mordechai ,  John Entwistle

IPC分类号： C12Q1/68 ,  C07H21/04

CPC分类号： C12Q1/6895 ,  C07H21/04 ,  C12Q2600/158

摘要： Disclosed are oligonucleotides useful in methods for determining whether a sample contains Cryptococcus neoformans, a causative agent for human cryptococcosis. These oligonucleotides, which have nucleotide sequences derived from a coding segment of the gene encoding the fungal specific transcription factor gene in Cryptococcus neoformans, are useful as forward and reverse primers for a polymerase chain reaction using nucleic acids from a biological sample as templates, and as probes for detecting any resultant amplicon. Detection of an amplicon indicates the sample contains Cryptococcus neoformans. Real-time PCR and detection using florescence resonance energy transfer is disclosed.

摘要翻译： 公开了用于确定样品是否含有新隐球菌隐球菌（人类隐球菌病的致病因子）的方法中的寡核苷酸。 这些寡核苷酸具有源自编码新型隐球菌中的真菌特异性转录因子基因的基因的编码区的核苷酸序列，可用作使用来自生物样品的核酸作为模板的聚合酶链反应的正向和反向引物，以及 用于检测任何所得扩增子的探针。 扩增子的检测表明样品含有新型隐球酵母。 公开了使用荧光共振能量转移的实时PCR和检测。

公开(公告)号：US08377656B2

公开(公告)日：2013-02-19

申请号：US13135022

申请日：2011-06-23

申请人： Melanie Feola ,  John Entwistle ,  Martin Adelson ,  Eli Mordechai

发明人： Melanie Feola ,  John Entwistle ,  Martin Adelson ,  Eli Mordechai

IPC分类号： C12P19/34 ,  C12Q1/68 ,  C07H21/04

CPC分类号： C12Q1/6895 ,  C07H21/04 ,  C12Q2600/158

摘要： Disclosed are oligonucleotides useful in methods for determining whether a sample contains Cryptococcus neoformans, a causative agent for human cryptococcosis. These oligonucleotides, which have nucleotide sequences derived from a coding segment of the gene encoding the fungal specific transcription factor gene in Cryptococcus neoformans, are useful as forward and reverse primers for a polymerase chain reaction using nucleic acids from a biological sample as templates, and as probes for detecting any resultant amplicon. Detection of an amplicon indicates the sample contains Cryptococcus neoformans. Real-time PCR and detection using florescence resonance energy transfer is disclosed.

公开(公告)号：US20120122092A1

公开(公告)日：2012-05-17

申请号：US13199093

申请日：2011-08-18

申请人： Melanie Feola ,  Martin Adelson ,  Eli Mordechai ,  Lisa Novak

发明人： Melanie Feola ,  Martin Adelson ,  Eli Mordechai ,  Lisa Novak

IPC分类号： C12Q1/68

CPC分类号： C12Q1/689 ,  Y02A50/57

摘要： Disclosed are oligonucleotides useful in methods for determining whether a sample contains Borrelia afzelii, a spirochete which is a causative agent of Lyme disease in humans. These oligonucleotides, which have nucleotide sequences derived from a coding segment of the gene encoding the p24 gene for the outer surface protein of Borrelia afzelii, are useful as forward and reverse primers for a polymerase chain reaction using nucleic acids from a biological sample as templates, and as probes for detecting any resultant amplicon. Detection of an amplicon indicates the sample contains Borrelia afzelii. Real-time PCR and detection using florescence resonance energy transfer is disclosed.

摘要翻译： 公开了用于确定样品是否含有人类的莱姆病的致病因子的螺旋体（Borrelia afzelii）的方法中使用的寡核苷酸。 这些寡核苷酸具有衍生自编码b24基因的基因的编码区段的核苷酸序列，用于使用来自生物样品的核酸作为模板的聚合酶链反应的正向和反向引物， 以及用于检测任何所得扩增子的探针。 扩增子的检测表明样品含有Borrelia afzelii。 公开了使用荧光共振能量转移的实时PCR和检测。

公开(公告)号：US07989186B2

公开(公告)日：2011-08-02

申请号：US12214317

申请日：2008-06-18

申请人： Melanie Feola ,  Martin Adelson ,  Eli Mordechai ,  John Entwistle

发明人： Melanie Feola ,  Martin Adelson ,  Eli Mordechai ,  John Entwistle

IPC分类号： C12P19/34

CPC分类号： C12Q1/6895 ,  C07H21/04 ,  C12Q2600/158

摘要： Disclosed are oligonucleotides useful in methods for determining whether a sample contains Cryptococcus neoformans, a causative agent for human cryptococcosis. These oligonucleotides, which have nucleotide sequences derived from a coding segment of the gene encoding the fungal specific transcription factor gene in Cryptococcus neoformans, are useful as forward and reverse primers for a polymerase chain reaction using nucleic acids from a biological sample as templates, and as probes for detecting any resultant amplicon. Detection of an amplicon indicates the sample contains Cryptococcus neoformans. Real-time PCR and detection using florescence resonance energy transfer is disclosed.

摘要翻译： 公开了用于确定样品是否含有新隐球菌隐球菌（人类隐球菌病的致病因子）的方法中的寡核苷酸。 这些寡核苷酸具有源自编码新型隐球菌中的真菌特异性转录因子基因的基因的编码区的核苷酸序列，可用作使用来自生物样品的核酸作为模板的聚合酶链反应的正向和反向引物，以及 用于检测任何所得扩增子的探针。 扩增子的检测表明样品含有新型隐球酵母。 公开了使用荧光共振能量转移的实时PCR和检测。

公开(公告)号：US20060246423A1

公开(公告)日：2006-11-02

申请号：US11343858

申请日：2006-01-31

申请人： Martin Adelson ,  Eli Mordechai

发明人： Martin Adelson ,  Eli Mordechai

IPC分类号： C12Q1/70 ,  G01N33/569 ,  G01N33/554 ,  A61M35/00 ,  G06F19/00 ,  C12M1/34 ,  C12Q1/04

CPC分类号： C12Q1/04

摘要： A method and kit related thereto are described for the collection and maintenance of detectability of a plurality of species of microbiological agents selected from the group consisting of bacteria, fungi, viruses, and protozoa, in a single gynecological sample which comprises providing transport media in a resealable container and instructions for preparation and handling of the gynecological sample and a written indication of the detectability of a plurality of species.

摘要翻译： 描述了与其相关的方法和试剂盒，用于在单个妇科样品中收集和维持选自细菌，真菌，病毒和原生动物的多种微生物剂的可检测性，其包括在 可重复密封的容器以及妇科样品的制备和处理说明以及多种物种的可检测性的书面指示。

公开(公告)号：US20140303072A1

公开(公告)日：2014-10-09

申请号：US14217606

申请日：2014-03-18

申请人： Scott Elliot Gygax ,  Christina Lim Overmyer ,  Lisa A. DeSalvia ,  Martin Adelson ,  Eli Mordechai

发明人： Scott Elliot Gygax ,  Christina Lim Overmyer ,  Lisa A. DeSalvia ,  Martin Adelson ,  Eli Mordechai

IPC分类号： C12Q1/68 ,  A61K31/5377 ,  A61K38/12 ,  A61K38/14

CPC分类号： C12Q1/689 ,  A61K31/5377 ,  A61K38/12 ,  A61K38/14 ,  C12Q1/6844 ,  C12Q2600/16

摘要： Disclosed are diagnostic methods for determining a subtype of methicillin-resistant Staphylococcus aureus (MRSA) in a biological sample of a mammal. Methods include providing a biological sample of the mammal, performing a PCR analysis of the biological sample, and analyzing the PCR amplicons with respect to their sizes so as to determine for type I, type II, type III, type IV or type V MRSA that may be present in the biological sample. Further example embodiments include using at least one mecA primer pair and/or using at least one Staphylococcus aureus nuc primer pair in the PCR analysis. Further disclosed are methods for screening populations for MRSA, and methods of treating a mammal testing positive for Type IV MRSA. Also disclosed are kits for determining a MRSA subtype in a mammal and isolated primers that may be used in the present methods and kits.

摘要翻译： 公开了用于确定哺乳动物生物样品中耐甲氧西林金黄色葡萄球菌（MRSA）亚型的诊断方法。 方法包括提供哺乳动物的生物样品，对生物样品进行PCR分析，并根据其大小对PCR扩增子进行分析，以确定I型，II型，III型，IV型或V型MRSA， 可能存在于生物样品中。 另外的实施例包括在PCR分析中使用至少一个mecA引物对和/或使用至少一种金黄色葡萄球菌引物对。 进一步披露的是用于筛选MRSA群体的方法以及治疗IV型MRSA阳性的哺乳动物的方法。 还公开了用于测定哺乳动物中MRSA亚型的试剂盒和可用于本发明方法和试剂盒的分离的引物。