公开(公告)号：US20050029195A1

公开(公告)日：2005-02-10

申请号：US10830935

申请日：2004-04-23

Applicant: Gary Gibson ,  Keith Hayes ,  Meng Heng ,  Csilla Kollar ,  Thomas Lane ,  Anthony Revis ,  Landon Steele

Inventor： Gary Gibson ,  Keith Hayes ,  Meng Heng ,  Csilla Kollar ,  Thomas Lane ,  Anthony Revis ,  Landon Steele

IPC: B01D15/08 ,  B01D15/36 ,  B01D15/38 ,  B01J20/10 ,  B01J20/286 ,  B01J20/32

CPC classification number: B01J20/3259 ,  B01D15/361 ,  B01D15/3804 ,  B01J20/103 ,  B01J20/14 ,  B01J20/286 ,  B01J20/3204 ,  B01J20/3242 ,  B01J20/3244 ,  B01J20/3257 ,  B01J2220/4887 ,  Y10T436/25375

Abstract: The present invention provides methods for separating one or more components of interest from a sample containing particulates and soluble materials. The method comprises the steps of: (a) filtering a sample through silica filter media whose surface silanol groups have reacted with one or more silanes, and (b) simultaneously capturing particulates and binding a soluble component to the silica filter media. The bound soluble component of interest is subsequently eluted from the silica filter media. In one embodiment of the invention, unwanted soluble materials are captured by the treated silica filter media and desired component of interest is recovered from the flow-through. In another embodiment of the invention, different components of interest are recovered from both the eluate and the flow-through. Preferred treated silica filter media are silane-treated rice hull ash or diatomaceous earth with functional quaternary ammonium group or functional sulphonate group. Particulates suitable for the present invention, for example, are microorganisms.

Abstract translation: 本发明提供了从含有微粒和可溶性材料的样品中分离出一种或多种感兴趣组分的方法。 该方法包括以下步骤：（a）通过其表面硅烷醇基团与一种或多种硅烷反应的二氧化硅过滤介质过滤样品，和（b）同时捕获微粒并将可溶组分结合到二氧化硅过滤介质上。 随后从二氧化硅过滤介质中洗脱所结合的可溶性成分。 在本发明的一个实施方案中，不希望的可溶物质被经处理的二氧化硅过滤介质捕获，并且所需的目的组分从流通中回收。 在本发明的另一个实施方案中，感兴趣的不同成分从洗脱液和流通液中回收。 优选的经处理的二氧化硅过滤介质是具有功能性季铵基团或官能磺酸盐基团的硅烷处理的稻壳灰或硅藻土。 适用于本发明的微粒例如是微生物。