公开(公告)号：US11008579B2

公开(公告)日：2021-05-18

申请号：US15084935

申请日：2016-03-30

Applicant: Keygene N.V.

Inventor： Paul Bundock ,  Michiel Theodoor Jan De Both ,  Frank Lhuissier

IPC: C12N15/82

Abstract: Method for targeted alteration of a duplex acceptor DNA sequence in a plant cell protoplast, comprising combining the duplex acceptor DNA sequence with a donor mutagenic nucleobase, wherein the duplex acceptor DNA sequence contains a first DNA sequence and a second DNA sequence which is the complement of the first DNA sequence and wherein the donor mutagenic nucleobase comprises at least one mismatch with respect to the duplex acceptor DNA sequence to be altered, preferably with respect to the first DNA sequence, wherein the method further comprises a step of introducing the donor mutagenic nucleobase into the cell protoplasts using polyethylene glycol (PEG) mediated transformation and the use of PEG protoplast transformation for enhancing the rate of targeted mutagenesis.

公开(公告)号：US20210123068A1

公开(公告)日：2021-04-29

申请号：US17142063

申请日：2021-01-05

Applicant: KEYGENE N.V.

Inventor： Paul BUNDOCK ,  Jeroen STUURMAN

IPC: C12N15/82 ,  A01H1/04 ,  C12N9/22

Abstract: The current disclosure relates to the field of plants, in particular to the fields of plant breeding and plant genetics. More particular, the disclosure concerns inventive methodology that may be useful in improving plant properties. In particular the invention may be useful in removing linkage drag. Also provided are plant and plant parts obtained with the method disclosed herein.

公开(公告)号：US20210024951A1

公开(公告)日：2021-01-28

申请号：US17037506

申请日：2020-09-29

Applicant: Keygene N.V.

Inventor： Diana RIGOLA ,  René Johannes Maria HOFSTEDE ,  Magdalena Barbara LASTDRAGER ,  Jamila Maguy CHAÏB

IPC: C12N15/82 ,  C12Q1/6895

Abstract: The present invention relates to genetic elements comprising powdery mildew-conferring QTLs derived from a plant of the species Cucumis melo, or powdery mildew-conferring part or variant thereof. The invention also relates to markers for identification of said QTLs, use thereof and methods for producing plants with increased resistance to powdery mildew and the plants thus obtained.

公开(公告)号：US20200185056A1

公开(公告)日：2020-06-11

申请号：US16517411

申请日：2019-07-19

Applicant: KEYGENE N.V.

Inventor： Michael Josephus Theresia VAN EIJK ,  Henricus Johannes Adam VAN DER POEL

IPC: G16B30/00 ,  C12Q1/6858 ,  C12Q1/6883 ,  C12Q1/6806 ,  C12Q1/6809 ,  C12Q1/6874 ,  C12N15/10 ,  C12Q1/6827 ,  C40B30/04

Abstract: The invention relates to a method for identifying one or more polymorphisms in nucleic acid samples, comprising: (a) performing a reproducible complexity reduction on a plurality of nucleic acid samples to provide a plurality of libraries of the nucleic acid samples comprising amplified fragments, wherein the reproducible complexity reduction comprises amplifying fragments of the nucleic acid samples using one or more primers to obtain the amplified fragments, and wherein the amplified fragments in each library comprise a unique identifier sequence to indicate origin of each library obtained by the reproducible complexity reduction; (b) combining the plurality of libraries to obtain a combined library and sequencing at least a portion of the combined library to obtain sequences; (c) aligning the sequences to obtain an alignment; and (d) identifying one or more polymorphisms in the plurality of nucleic acid samples.

公开(公告)号：US20200181698A1

公开(公告)日：2020-06-11

申请号：US16517502

申请日：2019-07-19

Applicant: KEYGENE N.V.

Inventor： Michael Josephus Theresia VAN EIJK ,  Anker Preben SØRENSEN ,  Marco Gerardus Maria VAN SCHRIEK

IPC: C12Q1/6874 ,  C12Q1/6876 ,  C12Q1/6883 ,  C12Q1/6855 ,  C12Q1/6869 ,  C12Q1/6827

Abstract: The invention relates to a method for the high throughput discovery, detection and genotyping of one or more genetic markers in one or more samples, comprising the steps of restriction endonuclease digest of DNA, adaptor-ligation, optional pre-amplification, selective amplification, pooling of the amplified products, sequencing the libraries with sufficient redundancy, clustering followed by identification of the genetic markers within the library and/or between libraries and determination of (co-)dominant genotypes of the genetic markers.

公开(公告)号：US20200181694A1

公开(公告)日：2020-06-11

申请号：US16516866

申请日：2019-07-19

Applicant: Keygene N.V.

Inventor： Michael Josephus Theresia Van Eijk ,  Rene Cornelis Josephus Hogers

IPC: C12Q1/6855 ,  C12Q1/6853

Abstract: The present invention relates to a high throughput method for the identification and detection of molecular markers wherein restriction fragments are generated and suitable adaptors comprising (sample-specific) identifiers are ligated. The adapter-ligated restriction fragments may be selectively amplified with adaptor compatible primers carrying selective nucleotides at their 3′ end. The amplified adapter-ligated restriction fragments are, at least partly, sequenced using high throughput sequencing methods and the sequence parts of the restriction fragments together with the sample-specific identifiers serve as molecular marker.

公开(公告)号：US20200095597A1

公开(公告)日：2020-03-26

申请号：US16491470

申请日：2018-03-07

Applicant: PureCircle USA Inc. ,  KeyGene N.V. ,  The Coca-Cola Company

Inventor： Avetik Markosyan ,  Seong Siang Ong ,  Runchun Jing ,  Tengfang Huang ,  Stephen Ezra Schauer ,  Fayaz Khazi ,  Indra Prakash ,  Alec Hayes

IPC: C12N15/82

Abstract: Stevia varieties with a high content of RebM, are disclosed Further provided are methods for producing Stevia plants having a high RebM content by negatively regulating certain genes selecting the resulting plants, and breeding with such plants to confer such desirable Reb M phenotypes to plant progeny.

公开(公告)号：US20200080110A1

公开(公告)日：2020-03-12

申请号：US16449326

申请日：2019-06-21

Applicant: Keygene N.V.

Inventor： Paul BUNDOCK

IPC: C12N15/90 ,  C12N15/10 ,  C12N9/22 ,  C12N15/11

Abstract: The current invention relates to methods of targeted genetic alteration in plant cells, as well as to plant cells and plants thus obtained. In the method, a Cpf1 protein and a crRNA is employed to provide for targeted alteration, in particular increased biallelic alteration, of a DNA duplex with increased efficacy.

公开(公告)号：US20190029202A1

公开(公告)日：2019-01-31

申请号：US15763710

申请日：2016-10-03

Applicant: KEYGENE N.V.

Inventor： Rik Hubertus Martinus OP DEN CAMP ,  Peter Johannes VAN DIJK ,  Anthony GALLARD

IPC: A01H1/08 ,  C07K14/415 ,  C12N15/82

Abstract: It was found that plants with loss of functional Msi2 protein due to a nucleotide polymorphism resulting in the introduction of a premature stop codon in the Msi2 protein, are able to induce haploid offspring after a cross to or with a wild type plant comprising a functional Msi2 protein. The invention relates to generation of haploid and doubled haploid plants.

公开(公告)号：US20180319904A1

公开(公告)日：2018-11-08

申请号：US15773024

申请日：2016-11-03

Applicant: Keygene N.V.

Inventor： Peter Johannes VAN DIJK ,  Rolf André MANK

IPC: C08C1/04 ,  C08C4/00 ,  C08C1/14 ,  C08C2/00

CPC classification number: C08C1/04 ,  B60C1/00 ,  C08C1/02 ,  C08C1/14 ,  C08C2/00 ,  C08C4/00 ,  C08L7/00 ,  C08L9/00

Abstract: The present invention relates to a method for obtaining rubber from rubber-containing plant material, the method comprising the steps of subjecting a composition comprising rubber-containing plant material and a fermentation culture to conditions allowing anaerobic fermentation, wherein the composition preferably has a water content of less than 95 wt. %. The fermentation preferably does not involve movement of the rubber-containing plant material (or parts thereof), and preferably the fermentation is performed in the absence of oxygen ventilation. Additionally, it is preferred that the fermentation culture is cycled through the composition by percolation. A further step of the method involves obtaining the rubber from the composition.