-
公开(公告)号：US20190017086A1
公开(公告)日：2019-01-17
申请号：US16145146
申请日：2018-09-27
发明人： Hironori OMURA , Hirokazu Sanada , Takako Yada , Ayaka Atsumi , Tetsunari Morita , Emi Ishimaru
IPC分类号： C12Q1/00 , C12N9/04 , G01N27/327 , C12N9/02 , C12Q1/32
摘要： The present invention provides members that produce on a large scale a coenzyme-linked glucose dehydrogenase which has excellent substrate-recognizing ability toward glucose while providing low action on maltose. The present invention relates to a polynucleotide encoding a soluble coenzyme-linked glucose dehydrogenase that catalyzes the oxidation of glucose in the presence of an electron acceptor and has an activity toward maltose of 5% or lower; a polypeptide encoded by the nucleotide sequence of the polynucleotide; a recombinant vector carrying the polynucleotide; a transformed cell produced using the recombinant vector; a method for producing a polypeptide comprising culturing the transformed cell and collecting from the cultivated products a polypeptide that links to FAD to exert the glucose dehydration activity; a method for determination of glucose using the polypeptide; a reagent composition for determination of glucose; and a biosensor.
-
公开(公告)号：US09957543B2
公开(公告)日：2018-05-01
申请号：US15079002
申请日：2016-03-23
发明人： Hironori Omura , Hirokazu Sanada , Takako Yada , Ayaka Atsumi , Tetsunari Morita , Emi Ishimaru
CPC分类号： C12Q1/006 , C12N9/0004 , C12N9/0006 , C12Q1/004 , C12Q1/32 , C12Y101/05 , C12Y101/9901 , G01N27/3271 , G01N2333/904
摘要： The present invention provides members that produce on a large scale a coenzyme-linked glucose dehydrogenase which has excellent substrate-recognizing ability toward glucose while providing low action on maltose. The present invention relates to a polynucleotide encoding a soluble coenzyme-linked glucose dehydrogenase that catalyzes the oxidation of glucose in the presence of an electron acceptor and has an activity toward maltose of 5% or lower; a polypeptide encoded by the nucleotide sequence of the polynucleotide; a recombinant vector carrying the polynucleotide; a transformed cell produced using the recombinant vector; a method for producing a polypeptide comprising culturing the transformed cell and collecting from the cultivated products a polypeptide that links to FAD to exert the glucose dehydration activity; a method for determination of glucose using the polypeptide; a reagent composition for determination of glucose; and a biosensor.
-
公开(公告)号：US08716442B2
公开(公告)日：2014-05-06
申请号：US12857880
申请日：2010-08-17
申请人： Ryo Takenaka , Michinari Honda , Hironori Omura
发明人： Ryo Takenaka , Michinari Honda , Hironori Omura
IPC分类号： C07K14/00
CPC分类号： H01M8/16 , A61B5/14532 , A61B5/1486 , C07K14/80 , C12N9/0006 , C12N15/62 , C12Q1/004 , Y02E60/527
摘要： The problem to be resolved is to provide an electron mediator and a fusion body with high affinity with an enzyme, a measuring method using extracellular secretion type cytochrome and an enzyme, an electrode, and a sensor.The present invention relates to an electron mediator for glucose oxidoreductase comprising extracellular secretion type cytochrome, a fusion body in which the electron mediator is fused with glucose oxidoreductase, a composition for glucose measurement including the electron mediator or fusion body, a gene encoding a new extracellular secretion type cytochrome, and a measurement method using extracellular secretion type cytochrome and an enzyme, an electrode, and a sensor.
摘要翻译： 要解决的问题是提供与酶具有高亲和力的电子介体和融合体，使用细胞外分泌型细胞色素的测定方法和酶，电极和传感器。 本发明涉及包含细胞外分泌型细胞色素的葡萄糖氧化还原酶的电子介体，其中电子介体与葡萄糖氧化还原酶融合的融合体，包括电子介体或融合体的葡萄糖测量用组合物，编码新的细胞外基因的基因 分泌型细胞色素，以及使用细胞外分泌型细胞色素和酶，电极和传感器的测定方法。
-
公开(公告)号：US20130203093A1
公开(公告)日：2013-08-08
申请号：US13777827
申请日：2013-02-26
发明人： Michinari HONDA , Sayaka Taki , Ryo Takenaka
IPC分类号： C12N9/00
CPC分类号： C12N9/0006 , C12Q1/006 , C12Q1/32 , C12Y101/01047 , C12Y101/9901
摘要： The invention provides a flavin-binding glucose dehydrogenase exhibiting reduced fluctuation of activity depending on temperature environment, and a method for measuring glucose concentration using the flavin-binding glucose dehydrogenase. The flavin-binding glucose dehydrogenase has the following properties (1) to (3): (1) activity: which exhibits glucose dehydrogenase activity in the presence of an electron acceptor; (2) substrate specificity: which exhibits an activity of 10% or less against maltose, D-galactose, D-fructose, sorbitol, lactose and sucrose when the activity against D-glucose is defined as 100%; and (3) temperature characteristics: which exhibits lower fluctuation of activity in a wide temperature range of 10 to 50° C.
摘要翻译： 本发明提供了一种黄色素结合性葡萄糖脱氢酶，其显示出根据温度环境降低的活性波动，以及使用黄素结合葡萄糖脱氢酶测量葡萄糖浓度的方法。 黄素结合葡萄糖脱氢酶具有以下性质（1）至（3）：（1）活性：在电子受体存在下显示葡萄糖脱氢酶活性; （2）底物特异性：当对D-葡萄糖的活性定义为100％时，其对麦芽糖，D-半乳糖，D-果糖，山梨糖醇，乳糖和蔗糖具有10％或更低的活性; 和（3）温度特性：在10〜50℃的较宽的温度范围内表现出较低的活性波动。
-
公开(公告)号：US20020098536A1
公开(公告)日：2002-07-25
申请号：US09988919
申请日：2001-11-19
发明人： Seiji Norinobu , Naoko Seo , Fumi Sato , Shoji Kaneko , Mitsumasa Mankura
IPC分类号： C12P033/00
摘要： A process for enzymatically producing, from a vegetable oil deodorizer distillate, a dietary sterol fatty acid ester superior in flavor qualities (e.g., color, odor and taste) and safety and containing no or little trans fatty acids, wherein the conditions for treatment of the starting material, synthetic reaction and subsequent purification are controlled so that the sterol fatty acid ester becomes applicable as a daily food material, a health food material or a pharmaceutical material. In the process, fatty acid esters, e.g., triacylglycerol, in the vegetable oil deodorizer distillate are previously degraded by hydrolysis with a chemical catalyst, fatty acids produced in the hydrolysis are removed by molecular distillation to give a sterol-containing fraction. The sterol-containing fraction is added with any fat and oil primarily comprising triacylglycerol. The mixture is used as the starting material, and the synthetic reaction is performed under strictly controlled conditions using a lipolytic enzyme. The resulting product undergoes several steps of purification process to provide qualities suitable for a food material.
摘要翻译： 一种从植物油除臭馏分中提取出具有优良风味品质（例如颜色，气味和味道）和安全性并且不含或少量反式脂肪酸的膳食甾醇脂肪酸酯的方法，其中处理 控制起始原料，合成反应和随后的纯化，使得固醇脂肪酸酯变得适用于日常食品材料，保健食品材料或药物材料。 在此过程中，植物油除臭馏分中的脂肪酸酯，例如三酰基甘油，预先通过用化学催化剂水解而降解，通过分子蒸馏除去在水解中产生的脂肪酸，得到含固醇馏分。 加入含甾醇的级分中主要包含三酰基甘油的任何脂肪和油。 使用该混合物作为起始原料，并且使用脂肪分解酶在严格控制的条件下进行合成反应。 所得产物经历几个步骤的纯化过程以提供适合食品材料的质量。
-
公开(公告)号：US20230049282A1
公开(公告)日：2023-02-16
申请号：US17399394
申请日：2021-08-11
发明人： Takeshi MOROSUGI , Akiomi OKADA
摘要： A frozen food is provided that requires neither addition of water nor large cookware such as a pot, an electromagnetic cooker and a gas stove; is easy to eat; can be stored for a long period; does not cause loosening and uneven heating of the noodle; and makes it possible to enjoy an aromatic and authentic taste in a simple manner only by heating in a microwave oven. It was found that a plurality of chunks of frozen soup having a specific size are placed at a higher position with respect to a frozen noodle, so that a frozen food that makes it possible to enjoy an authentic taste as a hot noodle simply by microwave cooking can be provided, and these findings have led to the present invention.
-
公开(公告)号：US20210238562A1
公开(公告)日：2021-08-05
申请号：US17049119
申请日：2019-04-15
IPC分类号： C12N9/04 , G01N33/535
摘要： The present invention addresses the problem of providing: a glucose dehydrogenase; a polynucleotide encoding the enzyme; a method for producing the enzyme; a method for measuring glucose using the enzyme; a measurement reagent composition; and a biosensor. The present invention pertains to a protein that has any of amino acid sequences (a), (b) and (c) and has a glucose dehydrogenase activity, etc.: (a) an amino acid sequence represented by SEQ ID NO: 3, 6, 15 or 16; (b) an amino acid sequence derived from the amino acid sequence represented by SEQ ID NO: 3, 6, 15 or 16 by deleting, substituting or adding 1-3 amino acids; and (c) an amino acid sequence having 90% or more identity to the amino acid sequence represented by SEQ ID NO: 3 or 15 or 85% or more identity to the amino acid sequence represented by SEQ ID NO: 6 or 16.
-
公开(公告)号：US10942187B2
公开(公告)日：2021-03-09
申请号：US16145509
申请日：2018-09-28
发明人： Daisuke Sato , Tomoko Nakatsuka , Hideyuki Aoki , Mikiko Kida , Kenta Yamada , Shoji Kaneko , Akimitsu Kugimiya
IPC分类号： G01N33/68 , G01N27/416
摘要： A method for selectively and easily quantifying the L-form and/or D-form amino acids to be measured using an aminoacyl tRNA synthetase (AARS) with high sensitivity, and an amino acid quantification kit. A method for quantifying amino acids (L-AA and/or D-AA) in a sample using an AARS, wherein the amino acids and the AARS are released from an aminoacyl AMP-AARS complex once formed, and they are used again for forming the aminoacyl AMP-AARS complex, so that reaction products such as pyrophosphoric acid to be measured can be ultimately produced up to a molar number larger than that of the amino acids contained in the sample, and an amino acid quantification kit for performing the method.
-
公开(公告)号：US10851398B2
公开(公告)日：2020-12-01
申请号：US16145196
申请日：2018-09-28
发明人： Hironori Omura , Hirokazu Sanada , Takako Yada , Ayaka Atsumi , Tetsunari Morita , Emi Ishimaru
摘要： The present invention provides members that produce on a large scale a coenzyme-linked glucose dehydrogenase which has excellent substrate-recognizing ability toward glucose while providing low action on maltose. The present invention relates to a polynucleotide encoding a soluble coenzyme-linked glucose dehydrogenase that catalyzes the oxidation of glucose in the presence of an electron acceptor and has an activity toward maltose of 5% or lower; a polypeptide encoded by the nucleotide sequence of the polynucleotide; a recombinant vector carrying the polynucleotide; a transformed cell produced using the recombinant vector; a method for producing a polypeptide comprising culturing the transformed cell and collecting from the cultivated products a polypeptide that links to FAD to exert the glucose dehydration activity; a method for determination of glucose using the polypeptide; a reagent composition for determination of glucose; and a biosensor.
-
公开(公告)号：US10808274B2
公开(公告)日：2020-10-20
申请号：US15928012
申请日：2018-03-21
发明人： Hironori Omura , Hirokazu Sanada , Takako Yada , Ayaka Atsumi , Tetsunari Morita , Emi Ishimaru
IPC分类号： C12Q1/00 , C12N9/02 , C12Q1/32 , C12N9/04 , G01N27/327
摘要： The present invention provides members that produce on a large scale a coenzyme-linked glucose dehydrogenase which has excellent substrate-recognizing ability toward glucose while providing low action on maltose. The present invention relates to a polynucleotide encoding a soluble coenzyme-linked glucose dehydrogenase that catalyzes the oxidation of glucose in the presence of an electron acceptor and has an activity toward maltose of 5% or lower; a polypeptide encoded by the nucleotide sequence of the polynucleotide; a recombinant vector carrying the polynucleotide; a transformed cell produced using the recombinant vector; a method for producing a polypeptide comprising culturing the transformed cell and collecting from the cultivated products a polypeptide that links to FAD to exert the glucose dehydration activity; a method for determination of glucose using the polypeptide; a reagent composition for determination of glucose; and a biosensor.
-
-
-
-
-
-
-
-
-
搜索结果
70 条记录 (耗时 0.025 秒)
