公开(公告)号：US06974697B2

公开(公告)日：2005-12-13

申请号：US10087346

申请日：2002-03-01

Applicant: Allen Comer ,  Lynn Allen-Hoffmann ,  Michael Hoffmann

Inventor： Allen Comer ,  Lynn Allen-Hoffmann ,  Michael Hoffmann

IPC: A61F2/10 ,  A61K45/00 ,  C12N5/00 ,  C12N5/02 ,  C12N5/071 ,  C12N5/08

CPC classification number: C12N5/0698 ,  C12N2500/36 ,  C12N2500/38 ,  C12N2500/90 ,  C12N2501/01 ,  C12N2501/11 ,  C12N2501/13 ,  C12N2501/385 ,  C12N2501/39 ,  C12N2501/60 ,  C12N2502/094 ,  C12N2502/1323 ,  C12N2510/00 ,  C12N2533/54

Abstract: The present invention relates to in vitro cultured skin substitutes, and in particular to in vitro cultured skin substitutes that have improved barrier function. In some embodiments, improved barrier function is a result of improved culture conditions, while in other embodiments, improved barrier function results from genetic modification of keratinocytes. Improved culture conditions to improve barrier function include organotypic culture in the presence of linoleic acid and/or linoleic acid at about 75% humidity. Suitable genetic modifications for improving barrier function includes transfection with a DNA construct capable of expressing GKLF.

Abstract translation: 本发明涉及体外培养的皮肤替代物，特别涉及具有改善的屏障功能的体外培养的皮肤代用品。 在一些实施方案中，改进的屏障功能是改善的培养条件的结果，而在其它实施方案中，角质形成细胞的遗传修饰改善的屏障功能结果。 提高屏障功能的改善的培养条件包括在约75％湿度下亚油酸和/或亚油酸存在下的器官培养。 用于改善屏障功能的合适的遗传修饰包括用能够表达GKLF的DNA构建体的转染。

公开(公告)号：US07407805B2

公开(公告)日：2008-08-05

申请号：US11235814

申请日：2005-09-27

Applicant: Allen Comer ,  Lynn Allen-Hoffmann ,  Michael Hoffmann

Inventor： Allen Comer ,  Lynn Allen-Hoffmann ,  Michael Hoffmann

IPC: A61K35/36 ,  C12N5/08 ,  C12N5/02 ,  C12N5/00

CPC classification number: C12N5/0698 ,  C12N2500/36 ,  C12N2500/38 ,  C12N2500/90 ,  C12N2501/01 ,  C12N2501/11 ,  C12N2501/13 ,  C12N2501/385 ,  C12N2501/39 ,  C12N2501/60 ,  C12N2502/094 ,  C12N2502/1323 ,  C12N2510/00 ,  C12N2533/54

Abstract: The present invention relates to in vitro cultured skin substitutes, and in particular to in vitro cultured skin substitutes that have improved barrier function. In some embodiments, improved barrier function is a result of improved culture conditions, while in other embodiments, improved barrier function results from genetic modification of keratinocytes. Improved culture conditions to improve barrier function include organotypic culture in the presence of linoleic acid and/or linoleic acid at about 75% humidity. Suitable genetic modifications for improving barrier function includes transfection with a DNA construct capable of expressing GKLF.

Abstract translation: 本发明涉及体外培养的皮肤替代物，特别涉及具有改善的屏障功能的体外培养的皮肤代用品。 在一些实施方案中，改进的屏障功能是改善的培养条件的结果，而在其它实施方案中，角质形成细胞的遗传修饰改善的屏障功能结果。 提高屏障功能的改善的培养条件包括在约75％湿度下亚油酸和/或亚油酸存在下的器官培养。 用于改善屏障功能的合适的遗传修饰包括用能够表达GKLF的DNA构建体的转染。