公开(公告)号：US5480789A

公开(公告)日：1996-01-02

申请号：US154143

申请日：1993-11-18

申请人： Ebrahim Firoozabady ,  Karol Robinson

发明人： Ebrahim Firoozabady ,  Karol Robinson

IPC分类号： C12N5/00 ,  C12N5/04 ,  C12N15/82 ,  C12N15/00 ,  A01H4/00 ,  A01H5/00 ,  C12N15/63

CPC分类号： C12N5/04 ,  C12N15/8205 ,  C12N5/0025 ,  C12N2510/00

摘要： Rose plant cells are transformed by incubation with Agrobacterium cells carrying an exogenous DNA sequence. The callus cells may be obtained from various tissue sources, including stamen filaments, leaf explants, and the like, and whole rose plants may be regenerated from the transformed callus cells. The exogenous DNA will be stably incorporated into the chromosomes of the regenerated rose plant which will be able to express gene(s) encoded by the DNA sequence.

摘要翻译： 通过与携带外源DNA序列的农杆菌细胞孵育来转化玫瑰植物细胞。 愈伤组织细胞可以从各种组织来源获得，包括雄蕊长丝，叶外植体等，并且整个玫瑰植物可以从转化的愈伤组织细胞再生。 外源DNA将稳定地并入再生的玫瑰植物的染色体中，其能够表达由DNA序列编码的基因。