摘要:
The present invention provides methods of transforming pineapple cells and plants with carotenoid biosynthetic polypeptide expression regulators that control the accumulation of carotenoids in the transformed cells, and in certain embodiments, the coloration of those cells. The invention also provides for the regeneration of pineapple plants from the transformed cells. In addition, the invention provides pineapple cells and plants that include introduced carotenoid biosynthetic polypeptide expression regulators.
摘要:
The invention provides novel regulatory polynucleotide sequences useful in plant genetic engineering applications, e.g., polynucleotides having transcriptional promoter or terminator activity, are provided. The invention also provides novel gene and polypeptide sequences, for example, genes corresponding to pineapple carotenoid biosynthesis proteins, e.g., carotenoid isomerase (ISO), phytoene synthase (PSY) and lycopene β-cyclase (LYC), which find use in producing plants with improved traits, e.g., improved nutritional value. In addition, related nucleic acids, e.g., vectors, and transformed plants that include one or more of these polynucleotides or polypeptides are also provided, as are related methods for producing such compositions.
摘要:
Rose plant cells are transformed by incubation with Agrobacterium cells carrying an exogenous DNA sequence. The callus cells may be obtained from various tissue sources, including stamen filaments, leaf explants, and the like, and whole rose plants may be regenerated from the transformed callus cells. The exogenous DNA will be stably incorporated into the chromosomes of the regenerated rose plant which will be able to express gene(s) encoded by the DNA sequence.
摘要:
Carnation plant material is transformed by cocultivation with Agrobacterium cells carrying an exogenous DNA sequence. In particular, by initiating callus formation on the plant material, transformed shoots may be produced in a suitable medium. Plantlets may be produced from the shoots by initiating root formation and subsequently transferring the rooted shoots to soil.
摘要:
A method for regeneration of sunflower plants via shoot induction from cotyledon explants prepared from non-germinated sunflower embryos is described. Several methods for preparing explants by dissection of mature and immature fully-formed embryos are provided. Explants are prepared to insure that substantially all of the induced shoots derive from single cotyledon cells. Appropriately prepared explants are cultured on a shoot induction medium containing a combination of BA and NAA. Induced shoots are rooted and the resulting plantlets are grown into mature sunflower plants. This regeneration method is efficient, rapid and employs a convenient explant source, sunflower seed. This regeneration method is particularly useful in combination with methods for introduction of foreign DNA into sunflower cells and tissue.
摘要:
A new pineapple variety named ‘Rosé’ is provided. Internal red or pink color with unique shell morphology and possibility of flowering control trait are traits of the new variety.
摘要:
A method for expressing insecticidal protein structural genes in cotton plant genomes is provided. In the preferred embodiments this invention comprises placing a structural gene for the Bacillus thuringiensis crystal protein under control of a plant or a T-DNA promoter and ahead of a polyadenylation site followed by insertion of said promoter/structural gene combination into a plant genome by utilizing an Agrobacterium tumefaciens Ti plasmid-based transformation system. The modified Ti plasmid is then used to transform recipient plant cells. Also provided are the plants and tissues produced by this method and bacterial strains, plasmids, and vectors useful for execution of this invention.
摘要:
The present invention is directed to methods for the genetic transformation of pineapple plant tissue with Agrobacterium. The present invention also provides for the regeneration of intact pineapple plants from the transformed tissue.