公开(公告)号：US20110190158A1

公开(公告)日：2011-08-04

申请号：US13064322

申请日：2011-03-18

申请人： Hajime Ikuta ,  Kiyokazu Takemura

发明人： Hajime Ikuta ,  Kiyokazu Takemura

IPC分类号： C40B30/04 ,  C40B40/06

CPC分类号： C12Q1/682 ,  C12Q1/6837 ,  C12Q2563/131 ,  C12Q2563/125 ,  C12Q2537/125 ,  C12Q2565/625 ,  C12Q2565/543 ,  C12Q2523/308

摘要： According to an aspect of the present invention, a pair of oligonucleotide strands are anchored onto the surface of a substrate by immobilizing one of the ends thereof onto the substrate. Each of the immobilized oligonucleotide strands is bound to a target nucleic acid sequence (single-stranded) having complementary sequences thereto to form a cross-linked structure on the substrate, thereby forming a finely reticulated space. Ligands are captured by this reticulated space through physical adsorption and caused to color with active substances reactive to the ligands. As a result of this, the present invention is capable of highly sensitively detecting even an exceedingly small concentration of a particular target nucleic acid sequence to be detected, at low cost and for a short time.

摘要翻译： 根据本发明的一个方面，通过将其一个端部固定在基底上，将一对寡核苷酸链锚定在基底的表面上。 固定的寡核苷酸链中的每一个都与具有互补序列的靶核酸序列（单链）结合以在基底上形成交联结构，由此形成精细的网状空间。 配体被这种网状空间通过物理吸附捕获，并使与对配体反应的活性物质着色。 结果，本发明能够以低成本和短时间高度灵敏地检测甚至是要检测的特定目标核酸序列的浓度极小。

公开(公告)号：US20100193431A1

公开(公告)日：2010-08-05

申请号：US12696082

申请日：2010-01-29

申请人： Hajime IKUTA ,  Tatsuo Sumino

发明人： Hajime IKUTA ,  Tatsuo Sumino

IPC分类号： C02F3/12 ,  C02F3/34

CPC分类号： C02F3/302 ,  C02F1/66

摘要： A method for producing a nitrite type nitrification-reactive sludge includes the step of subjecting an activated sludge to alkali treatment so that the pH of the activated sludge containing at least the ammonium oxidizing bacteria and nitrite oxidizing bacteria can be 10 or higher, in order to accumulate ammonium oxidizing bacteria with higher priority in the activated sludge.

摘要翻译： 一种生产亚硝酸盐型硝化反应性污泥的方法包括以下步骤：使活性污泥进行碱处理，使至少含有氨氧化细菌和亚硝酸盐氧化细菌的活性污泥的pH为10以上，以便 在活性污泥中积聚较高优先级的氨氧化细菌。