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公开(公告)号：US08263334B2
公开(公告)日：2012-09-11
申请号：US13064322
申请日：2011-03-18
申请人： Hajime Ikuta , Kiyokazu Takemura
发明人： Hajime Ikuta , Kiyokazu Takemura
CPC分类号： C12Q1/682 , C12Q1/6837 , C12Q2563/131 , C12Q2563/125 , C12Q2537/125 , C12Q2565/625 , C12Q2565/543 , C12Q2523/308
摘要： According to an aspect of the present invention, a pair of oligonucleotide strands are anchored onto the surface of a substrate by immobilizing one of the ends thereof onto the substrate. Each of the immobilized oligonucleotide strands is bound to a target nucleic acid sequence (single-stranded) having complementary sequences thereto to form a cross-linked structure on the substrate, thereby forming a finely reticulated space. Ligands are captured by this reticulated space through physical adsorption and caused to color with active substances reactive to the ligands. As a result of this, the present invention is capable of highly sensitively detecting even an exceedingly small concentration of a particular target nucleic acid sequence to be detected, at low cost and for a short time.
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公开(公告)号：US20080248562A1
公开(公告)日：2008-10-09
申请号：US10586730
申请日：2005-01-20
申请人： Tadashi Sano , Ryo Miyake , Yasuhiko Sasaki , Tatsuo Sumino , Kazuichi Isaka , Hajime Ikuta
发明人： Tadashi Sano , Ryo Miyake , Yasuhiko Sasaki , Tatsuo Sumino , Kazuichi Isaka , Hajime Ikuta
IPC分类号： C12M1/00
CPC分类号： G01N15/10 , G01N2015/0088
摘要： A microorganism separation device includes: sample solution supply means (30) for supplying a sample solution (40) stored within a sample solution reservoir (32) to a first flow path (12); a microorganism sensor (22) that is capable of detecting a monadelphous microorganism in the sample solution (40) that passes through the first flow path (12); a controller (24) that stops to supply the sample solution (40) to the first flow path (12) and discharges the detected microorganism together with the sample solution (40) from a termination side of the first flow path (12) on the basis of a detection result of the microorganism by the microorganism sensor (22); and an acceptor (52) that receives a droplet (28) of the sample solution (40) that is discharged from the termination side of the first flow path (12).
摘要翻译： 微生物分离装置包括：样品溶液供给装置（30），用于将存储在样品溶液储存器（32）内的样品溶液（40）供应到第一流动路径（12）; 微生物传感器（22），其能够检测通过所述第一流路（12）的所述样品溶液（40）中的单位微生物; 控制器（24），停止向第一流路（12）供应样品溶液（40），并将检测到的微生物与样品溶液（40）一起从第一流路（12）的终止侧排出到 微生物传感器（22）对微生物的检测结果的基础; 以及接收从第一流路（12）的终端侧排出的样品溶液（40）的液滴（28）的受体（52）。
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3.发明申请公开(公告)号：US20090162860A1
公开(公告)日：2009-06-25
申请号：US12289512
申请日：2008-10-29
申请人： Hajime Ikuta , Kiyokazu Takemura
发明人： Hajime Ikuta , Kiyokazu Takemura
IPC分类号： C12Q1/68
CPC分类号： C12Q1/682 , C12Q1/6837 , C12Q2563/131 , C12Q2563/125 , C12Q2537/125 , C12Q2565/625 , C12Q2565/543 , C12Q2523/308
摘要： According to an aspect of the present invention, a pair of oligonucleotide strands are anchored onto the surface of a substrate by immobilizing one of the ends thereof onto the substrate. Each of the immobilized oligonucleotide strands is bound to a target nucleic acid sequence (single-stranded) having complementary sequences thereto to form a cross-linked structure on the substrate, thereby forming a finely reticulated space. Ligands are captured by this reticulated space through physical adsorption and caused to color with active substances reactive to the ligands. As a result of this, the present invention is capable of highly sensitively detecting even an exceedingly small concentration of a particular target nucleic acid sequence to be detected, at low cost and for a short time.
摘要翻译： 根据本发明的一个方面，通过将其一个端部固定在基底上，将一对寡核苷酸链锚定在基底的表面上。 固定的寡核苷酸链中的每一个都与具有互补序列的靶核酸序列（单链）结合以在基底上形成交联结构，由此形成精细的网状空间。 配体被这种网状空间通过物理吸附捕获，并使与对配体反应的活性物质着色。 结果，本发明能够以低成本和短时间高度灵敏地检测甚至是要检测的特定目标核酸序列的浓度极小。
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公开(公告)号：US20080098092A1
公开(公告)日：2008-04-24
申请号：US11755944
申请日：2007-05-31
申请人： Tadashi Sano , Yasuhiko Sasaki , Hajime Ikuta , Kazuichi Isaka , Tatsuo Sumino , Shigenori Togashi , Tetsuro Miyamoto
发明人： Tadashi Sano , Yasuhiko Sasaki , Hajime Ikuta , Kazuichi Isaka , Tatsuo Sumino , Shigenori Togashi , Tetsuro Miyamoto
摘要： A microorganism separation system comprising a sample solution container 34 containing microorganisms, a separator 1, and a receiver 47, designed to separate microorganisms from the sample solution; further comprising a microorganism detection sensor and a plate 49 which has a plurality of receivers 47 connected to each other and an identification indicator, wherein when the microorganism detection sensor judges that a microorganism has passed, supply of the sample solution is stopped, the detected microorganism is discharged together with the sample solution, and then the solution starts to be injected into the receiver; and, the number of times microorganisms are detected during the time period from the start of the injection to the end is recognized as separation quantity; and then as separation information, the separation quantity, number for a receiver 47 into which a microorganism was injected for each identification indicator, signal waveform sent from the microorganism detection sensor at the separation, date and temperature are stored.
摘要翻译： 一种微生物分离系统，包括含有微生物的样品溶液容器34，分离器1和接收器47，设计用于将微生物与样品溶液分离; 进一步包括微生物检测传感器和板49，其具有彼此连接的多个接收器47和识别指示器，其中当微生物检测传感器判断微生物已经通过时，停止供应样品溶液，检测到的微生物 与样品溶液一起放出，然后溶液开始注入接收器; 并且在从注射开始到结束的时间段期间检测到微生物的次数被识别为分离量; 然后作为分离信息，存储分离量，针对每个识别指示符注入微生物的接收器47的数量，在分离，日期和温度从微生物检测传感器发送的信号波形。
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5.发明授权公开(公告)号：US08227222B2
公开(公告)日：2012-07-24
申请号：US12532941
申请日：2008-03-26
申请人： Tatsuo Sumino , Naoki Abe , Hajime Ikuta , Naomichi Mori , Yutaka Mitani , Yasuhiro Oki
发明人： Tatsuo Sumino , Naoki Abe , Hajime Ikuta , Naomichi Mori , Yutaka Mitani , Yasuhiro Oki
IPC分类号： C12P5/02
CPC分类号： C12M21/04 , C12M23/58 , C12M25/16 , C12M41/26 , C12N11/04 , C12P3/00 , C12R1/01 , Y02E50/343
摘要： The hydrogen-producing method of the an aspect of the present invention is a method for producing hydrogen in which hydrogen is produced from an organic matter using a microorganism, characterized by using pellets on which hydrogen-producing acid-resistant bacteria are entrapped and immobilized, producing hydrogen by bringing the pellets into contact and react with the organic matter in an environment of a pH of 4 to 6. The inventors of the present invention have obtained a finding that a hydrogen-producing bacteria are entrapped and immobilized, so that the optimum pH of the hydrogen-producing bacterium shifts to low range. The present invention is made based on the above finding and uses pellets on which the hydrogen-producing bacteria are entrapped and immobilized, so that the hydrogen-producing bacterium is activated at a low pH range of 4 to 6 to produce hydrogen. Therefore, the effects of contaminated bacteria that consume hydrogen are less, and the yield of hydrogen can be improved.
摘要翻译： 本发明的一个方面的制氢方法是使用微生物从有机物中产生氢的氢的制造方法，其特征在于，使用在其上捕获并固定产氢耐酸细菌的粒料， 通过使颗粒接触并在pH为4至6的环境中与有机物质反应来生产氢。本发明的发明人已经获得了产生氢的细菌被捕获和固定的发现，使得最佳 产氢细菌的pH转变到低范围。 本发明是基于上述发现进行的，并且使用其上产生氢的细菌被捕获和固定的颗粒，使得产生氢的细菌在4至6的低pH范围内活化以产生氢。 因此，消耗氢的污染细菌的影响较小，可以提高氢的产率。
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6.发明授权公开(公告)号：US08221974B2
公开(公告)日：2012-07-17
申请号：US12289512
申请日：2008-10-29
申请人： Hajime Ikuta , Kiyokazu Takemura
发明人： Hajime Ikuta , Kiyokazu Takemura
CPC分类号： C12Q1/682 , C12Q1/6837 , C12Q2563/131 , C12Q2563/125 , C12Q2537/125 , C12Q2565/625 , C12Q2565/543 , C12Q2523/308
摘要： According to an aspect of the present invention, a pair of oligonucleotide strands are anchored onto the surface of a substrate by immobilizing one of the ends thereof onto the substrate. Each of the immobilized oligonucleotide strands is bound to a target nucleic acid sequence (single-stranded) having complementary sequences thereto to form a cross-linked structure on the substrate, thereby forming a finely reticulated space. Ligands are captured by this reticulated space through physical adsorption and caused to color with active substances reactive to the ligands. As a result of this, the present invention is capable of highly sensitively detecting even an exceedingly small concentration of a particular target nucleic acid sequence to be detected, at low cost and for a short time.
摘要翻译： 根据本发明的一个方面，通过将其一个端部固定在基底上，将一对寡核苷酸链锚定在基底的表面上。 固定的寡核苷酸链中的每一个都与具有互补序列的靶核酸序列（单链）结合以在基底上形成交联结构，由此形成精细的网状空间。 配体被这种网状空间通过物理吸附捕获，并使与对配体反应的活性物质着色。 结果，本发明能够以低成本和短时间高度灵敏地检测甚至是要检测的特定目标核酸序列的浓度极小。
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7.发明申请METHOD AND APPARATUS FOR PRODUCING HYDROGEN AND MICROORGANISM-IMMOBILIZATION PELLETS USED IN THE SAME 失效用于生产与其相同的氢和微生物固定颗粒的方法和装置公开(公告)号：US20100136643A1
公开(公告)日：2010-06-03
申请号：US12532941
申请日：2008-03-26
申请人： Tatsuo Sumino , Naoki Abe , Hajime Ikuta , Naomichi Mori , Yutaka Mitani , Yasuhiro Oki
发明人： Tatsuo Sumino , Naoki Abe , Hajime Ikuta , Naomichi Mori , Yutaka Mitani , Yasuhiro Oki
CPC分类号： C12M21/04 , C12M23/58 , C12M25/16 , C12M41/26 , C12N11/04 , C12P3/00 , C12R1/01 , Y02E50/343
摘要： The hydrogen-producing method of the an aspect of the present invention is a method for producing hydrogen in which hydrogen is produced from an organic matter using a microorganism, characterized by using pellets on which hydrogen-producing acid-resistant bacteria are entrapped and immobilized, producing hydrogen by bringing the pellets into contact and react with the organic matter in an environment of a pH of 4 to 6. The inventors of the present invention have obtained a finding that a hydrogen-producing bacteria are entrapped and immobilized, so that the optimum pH of the hydrogen-producing bacterium shifts to low range. The present invention is made based on the above finding and uses pellets on which the hydrogen-producing bacteria are entrapped and immobilized, so that the hydrogen-producing bacterium is activated at a low pH range of 4 to 6 to produce hydrogen. Therefore, the effects of contaminated bacteria that consume hydrogen are less, and the yield of hydrogen can be improved.
摘要翻译： 本发明的一个方面的制氢方法是使用微生物从有机物中产生氢的氢的制造方法，其特征在于，使用在其上捕获并固定产氢耐酸细菌的粒料， 通过使颗粒接触并在pH为4至6的环境中与有机物质反应来生产氢。本发明的发明人已经获得了产生氢的细菌被捕获和固定的发现，使得最佳 产氢细菌的pH转变到低范围。 本发明是基于上述发现进行的，并且使用其上产生氢的细菌被捕获和固定的颗粒，使得产生氢的细菌在4至6的低pH范围内活化以产生氢。 因此，消耗氢的污染细菌的影响较小，可以提高氢的产率。
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公开(公告)号：US07691990B2
公开(公告)日：2010-04-06
申请号：US11629788
申请日：2005-06-14
申请人： Zuyi Zhang , Toshiya Yuasa , Yoshinori Kotani , Kazumi Tanaka , Hajime Ikuta , Kunio Miyasaka
发明人： Zuyi Zhang , Toshiya Yuasa , Yoshinori Kotani , Kazumi Tanaka , Hajime Ikuta , Kunio Miyasaka
CPC分类号： B01J20/06 , B01J20/103 , B01J20/28016 , B01J20/28033 , B01J20/3274 , B01J2220/42 , B01J2220/4856 , B01J2220/58
摘要： Provided is a structure designed for adsorption, which is suitable for removing dioxin and dioxin-like substances from leachate and ground water from polluted soils or garbage, washing effluent from garbage incinerators and so on that contain DNA intercalators, particularly, dioxin and dioxin-like substances. The structure designed for adsorption is a structure designed for adsorption having an adsorbing layer containing, as a constituent, a DNA complex containing a DNA-binding protein, DNA including double strand DNA and a carrier, which can selectively remove DNA intercalators with high efficiency from water, gas and so on containing them.
摘要翻译： 提供了一种用于吸附的结构，适用于从污染土壤或垃圾中的渗滤液和地下水中除去二恶英和二恶英样物质，从含有DNA嵌入剂的垃圾焚烧炉排出的洗涤液等，特别是二恶英和二恶英样物质 物质 设计用于吸附的结构是设计用于吸附的结构，其吸收层含有含有DNA结合蛋白的DNA复合物，包含双链DNA和载体的DNA，其可以从高效率选择性地除去DNA嵌入剂 水，气等都含有它们。
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公开(公告)号：US20070148675A1
公开(公告)日：2007-06-28
申请号：US11629788
申请日：2005-06-14
申请人： Zuyi Zhang , Toshiya Yuasa , Yoshinori Kotani , Kazumi Tanaka , Hajime Ikuta , Kunio Miyasaka
发明人： Zuyi Zhang , Toshiya Yuasa , Yoshinori Kotani , Kazumi Tanaka , Hajime Ikuta , Kunio Miyasaka
CPC分类号： B01J20/06 , B01J20/103 , B01J20/28016 , B01J20/28033 , B01J20/3274 , B01J2220/42 , B01J2220/4856 , B01J2220/58
摘要： Provided is a structure designed for adsorption, which is suitable for removing dioxin and dioxin-like substances from leachate and ground water from polluted soils or garbage, washing effluent from garbage incinerators and so on that contain DNA intercalators, particularly, dioxin and dioxin-like substances. The structure designed for adsorption is a structure designed for adsorption having an adsorbing layer containing, as a constituent, a DNA complex containing a DNA-binding protein, DNA including double strand DNA and a carrier, which can selectively remove DNA intercalators with high efficiency from water, gas and so on containing them.
摘要翻译： 提供了一种用于吸附的结构，适用于从污染土壤或垃圾中的渗滤液和地下水中除去二恶英和二恶英样物质，从含有DNA嵌入剂的垃圾焚烧炉排出的洗涤液等，特别是二恶英和二恶英样物质 物质 设计用于吸附的结构是设计用于吸附的结构，其吸收层含有含有DNA结合蛋白的DNA复合物，包含双链DNA和载体的DNA，其可以从高效率选择性地除去DNA嵌入剂 水，气等都含有它们。
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公开(公告)号：US08323487B2
公开(公告)日：2012-12-04
申请号：US12696082
申请日：2010-01-29
申请人： Hajime Ikuta , Tatsuo Sumino
发明人： Hajime Ikuta , Tatsuo Sumino
IPC分类号： C02F3/00
摘要： A method for producing a nitrite type nitrification-reactive sludge includes the step of subjecting an activated sludge to alkali treatment so that the pH of the activated sludge containing at least the ammonium oxidizing bacteria and nitrite oxidizing bacteria can be 10 or higher, in order to accumulate ammonium oxidizing bacteria with higher priority in the activated sludge.
摘要翻译： 一种生产亚硝酸盐型硝化反应性污泥的方法包括以下步骤：使活性污泥进行碱处理，使至少含有氨氧化细菌和亚硝酸盐氧化细菌的活性污泥的pH为10以上，以便 在活性污泥中积聚较高优先级的氨氧化细菌。
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