公开(公告)号：US20130053354A1

公开(公告)日：2013-02-28

申请号：US13643803

申请日：2011-04-25

Applicant: Jae-Jin Han

Inventor： Jae-Jin Han

IPC: A61K31/618 ,  A61P1/02 ,  A61K31/235 ,  A61P19/08 ,  A61K31/60 ,  A61K31/192

CPC classification number: A61C8/0006 ,  A61L27/50 ,  A61L2430/02 ,  A61L2430/12 ,  C07C65/03 ,  C07C69/84

Abstract: Provided is a bone-transplant or bone-filling composition, which comprises a dihydroxybenzoic acid derivative which has been newly demonstrated to increase the activity of osteoblasts and so induce bone formation.

Abstract translation: 提供了一种骨移植或骨填充组合物，其包含已被新证实增加成骨细胞活性并因此诱导骨形成的二羟基苯甲酸衍生物。

公开(公告)号：US20140093955A1

公开(公告)日：2014-04-03

申请号：US14090768

申请日：2013-11-26

Applicant: Jae-Jin HAN

Inventor： Jae-Jin HAN

IPC: C12N5/071

CPC classification number: C12N5/0602 ,  C12N5/0018 ,  C12N2500/76

Abstract: Provided is a method for culturing cells, which prevents the possibility of mycoplasma contamination by culturing the cells using biomass extract obtained by culturing a strain with amphidinol productivity, preferably a strain such as Amphidinium klebsii, Amphididinium carterae, and the like belonging to Dinoflagellates, or using a fraction obtained from the extract; and a method for removing mycoplasma contamination of the cells by culturing the cells contaminated by mycoplasma using the amphidinol derivatives. In addition, provided is a method for removing mycoplasma contamination of the cells, including a combination of single cell-isolating enzyme solution treatment and cell aggregate removal.

Abstract translation: 本发明提供一种培养细胞的方法，其通过利用通过培养具有阿奇霉素生产力的菌株获得的生物质提取物优选培养细胞来防止支原体污染的可能性，优选菌株如属于鞭毛藻的as螨属（Amphidinium klebsii），镰孢菌纲（Amphididinium carterae）等，或 使用从提取物获得的级分; 以及通过使用二甲啶醇衍生物培养受支原体污染的细胞来去除细胞的支原体污染的方法。 此外，提供了除去细胞的支原体污染的方法，包括单细胞分离酶溶液处理和细胞聚集体去除的组合。