公开(公告)号：US20050181353A1

公开(公告)日：2005-08-18

申请号：US10780349

申请日：2004-02-17

申请人： Galla Rao ,  Melissa Herman ,  Herman Rutner ,  Leon Terstappen

发明人： Galla Rao ,  Melissa Herman ,  Herman Rutner ,  Leon Terstappen

IPC分类号： A01N1/02 ,  A61K31/198 ,  A61K31/727

CPC分类号： A01N1/02 ,  A01N1/0226 ,  A61K31/198 ,  A61K31/727

摘要： Compositions and methods for stabilizing rare cells in blood specimens, preserving the quality of blood specimens, and also serving as cell fixatives are disclosed which minimize losses of target cells (for example, circulating tumor cells) and formation of debris and aggregates from target cells, non-target cells and plasma components, thereby allowing more accurate analysis and classification of circulating tumor cells (CTC) and, ultimately, of tumor burdens in cancer patients. Stabilization of specimens is particularly desirable in protocols requiring rare cell enrichment from blood specimens drawn from cancer patients. Exposure of such specimens to potentially stressful conditions encountered, for example, in normal processing, mixing, shaking, delays due to transporting the blood, has been observed to not only diminish the number of CTC but also to generate debris and aggregates in the blood specimens that were found to interfere with accurate enumeration of target cells, if present. Stabilizers are necessary to discriminate between in vivo CTC disintegration and in vitro sample degredation.

摘要翻译： 公开了用于稳定血液标本中稀有细胞，保持血液标本质量以及用作细胞固定剂的组合物和方法，其使靶细胞（例如循环肿瘤细胞）的损失最小化，并从靶细胞形成碎片和聚集体， 非靶细胞和血浆成分，从而允许更准确地分析和分类循环肿瘤细胞（CTC），最终导致癌症患者的肿瘤负担。 在从癌症患者抽取的血液标本中需要罕见细胞富集的方案中，标本的稳定是特别需要的。 已经观察到这些样品暴露于例如在正常加工，混合，摇动，由于运送血液引起的延迟所遇到的潜在压力条件下，不仅减少了CTC的数量，而且在血样中产生碎片和聚集体 被发现干扰靶细胞的准确计数（如果存在）。 鉴别体内CTC分解和体外样品降解是必需的稳定剂。

公开(公告)号：US20050003464A1

公开(公告)日：2005-01-06

申请号：US10612144

申请日：2003-07-02

申请人： Arjan Tibbe ,  Jan Greve ,  Leon Terstappen ,  Bart De Grooth

发明人： Arjan Tibbe ,  Jan Greve ,  Leon Terstappen ,  Bart De Grooth

IPC分类号： C12M1/34 ,  G01N33/543 ,  G01N33/574

CPC分类号： G01N33/54326

摘要： Devices and methods for automated collection and image analysis are disclosed that enable identification or classification of microscopic objects aligned or deposited on surfaces. Such objects, e.g. detectably labeled rare target cells, are magnetically or non-magnetically immobilized and subjected to automated laser scanning to generate sequential digitized x-y sub-images or partial images of target and non-target objects that are combined to form reconstructed full images, thereby allowing detection, enumeration, differentiation and characterization of imaged objects on the basis of size, morphology and immunophenotype.

摘要翻译： 公开了用于自动收集和图像分析的装置和方法，其能够识别或分类对准或沉积在表面上的微观物体。 这些物体，例如。 可检测标记的稀有目标细胞是磁性或非磁性固定的，并进行自动激光扫描以产生被组合以形成重建的全部图像的目标和非目标对象的顺序数字化的xy子图像或部分图像，从而允许检测， 基于大小，形态和免疫表型的成像物体的计数，分化和表征。