公开(公告)号：US20060257847A1

公开(公告)日：2006-11-16

申请号：US11447562

申请日：2006-06-06

申请人： Tycho Scholtens ,  Leon Terstappen ,  Arjan Tibbe

发明人： Tycho Scholtens ,  Leon Terstappen ,  Arjan Tibbe

IPC分类号： C12Q1/00 ,  C12M1/34

CPC分类号： C12M33/00 ,  Y10T436/25 ,  Y10T436/25375

摘要： Apparatuses and methods for separating, immobilizing, and quantifying biological substances from within a fluid medium. Biological substances are observed by employing a vessel (6) having a chamber therein, the vessel comprising a transparent collection wall (5). A high internal gradient magnetic capture structure may be on the transparent collection wall (5), magnets (3) create an externally-applied force for transporting magnetically responsive material toward the transparent collection wall (5). V-shaped grooves on the inner surface of the viewing face of the chamber provide uniform. The invention is also useful in conducting quantitative analysis and sample preparation in conjunction with automated cell enumeration techniques.

摘要翻译： 从流体介质中分离，固定和定量生物物质的装置和方法。 通过使用其中具有室的容器（6）观察生物物质，所述容器包括透明收集壁（5）。 高的内部梯度磁捕获结构可以在透明收集壁（5）上，磁体（3）产生用于将磁响应材料传送到透明收集壁（5）的外部施加的力。 在腔室的观察面的内表面上的V形凹槽提供均匀的。 本发明还可用于结合自动细胞计数技术进行定量分析和样品制备。

公开(公告)号：US20060024756A1

公开(公告)日：2006-02-02

申请号：US10903798

申请日：2004-07-30

申请人： Arjan Tibbe ,  Jan Greve ,  Dhanesh Gohel ,  Erik Droog ,  Leon Terstappen

发明人： Arjan Tibbe ,  Jan Greve ,  Dhanesh Gohel ,  Erik Droog ,  Leon Terstappen

IPC分类号： G01N33/53

CPC分类号： G01N15/1475 ,  G01N15/1463 ,  G01N33/5094 ,  G01N33/54333 ,  G01N2015/008 ,  G01N2015/1486 ,  Y10T436/101666 ,  Y10T436/25375

摘要： The enumeration of cells in fluids by flow cytometry is widely used across many disciplines such as assessment of leukocyte subsets in different bodily fluids or of bacterial contamination in environmental samples, food products and bodily fluids. For many applications the cost, size and complexity of the instruments prevents wider use, for example, CD4 analysis in HIV monitoring in resource-poor countries. The novel device, methods and algorithms disclosed herein largely overcome these limitations. Briefly, all cells in a biological sample are fluorescently labeled, but only the target cells are also magnetically labeled. The labeled sample, in a chamber or cuvet, is placed between two wedge-shaped magnets to selectively move the magnetically labeled cells to the observation surface of the cuvet. An LED illuminates the cells and a CCD camera captures the images of the fluorescent light emitted by the target cells. Image analysis performed with a novel algorithm provides a count of the cells on the surface that can be related to the target cell concentration of the original sample. The compact cytometer system provides a rugged, affordable and easy-to-use technique, which can be used in remote locations.

摘要翻译： 通过流式细胞术对流体细胞的计数被广泛应用于许多学科，例如评估不同体液中的白细胞亚群或环境样品，食品和体液中的细菌污染。 对于许多应用，仪器的成本，尺寸和复杂性阻止了更广泛的使用，例如资源贫乏国家的艾滋病毒监测中的CD4分析。 本文公开的新颖的装置，方法和算法在很大程度上克服了这些限制。 简言之，生物样品中的所有细胞都被荧光标记，但只有靶细胞也被磁性标记。 将标记的样品置于室或铜管中，放置在两个楔形磁体之间，以选择性地将磁性标记的细胞移动到铜管的观察表面。 LED照亮细胞，CCD相机捕获由靶细胞发射的荧光的图像。 用新算法进行的图像分析提供了与原始样品的靶细胞浓度相关的表面上的细胞计数。 紧凑型细胞仪系统提供坚固，经济实惠且易于使用的技术，可用于偏远地区。

公开(公告)号：US20110044527A1

公开(公告)日：2011-02-24

申请号：US12917000

申请日：2010-11-01

申请人： Arjan Tibbe ,  Jan Greve ,  Dhanesh Gohel ,  Erik Droog ,  Leon W.M.M. Terstappen

发明人： Arjan Tibbe ,  Jan Greve ,  Dhanesh Gohel ,  Erik Droog ,  Leon W.M.M. Terstappen

IPC分类号： G06K9/00

CPC分类号： G01N15/1475 ,  G01N15/1463 ,  G01N33/5094 ,  G01N33/54333 ,  G01N2015/008 ,  G01N2015/1486 ,  Y10T436/101666 ,  Y10T436/25375

摘要： The enumeration of cells in fluids by flow cytometry is widely used across many disciplines such as assessment of leukocyte subsets in different bodily fluids or of bacterial contamination in environmental samples, food products and bodily fluids. For many applications the cost, size and complexity of the instruments prevents wider use, for example, CD4 analysis in HIV monitoring in resource-poor countries. The novel device, methods and algorithms disclosed herein largely overcome these limitations. Briefly, all cells in a biological sample are fluorescently labeled, but only the target cells are also magnetically labeled. The labeled sample, in a chamber or cuvet, is placed between two wedge-shaped magnets to selectively move the magnetically labeled cells to the observation surface of the cuvet. An LED illuminates the cells and a CCD camera captures the images of the fluorescent light emitted by the target cells. Image analysis performed with a novel algorithm provides a count of the cells on the surface that can be related to the target cell concentration of the original sample. The compact cytometer system provides a rugged, affordable and easy-to-use technique, which can be used in remote locations.

摘要翻译： 通过流式细胞术对流体细胞的计数被广泛应用于许多学科，例如评估不同体液中的白细胞亚群或环境样品，食品和体液中的细菌污染。 对于许多应用，仪器的成本，尺寸和复杂性阻止了更广泛的使用，例如资源贫乏国家的艾滋病毒监测中的CD4分析。 本文公开的新颖的装置，方法和算法在很大程度上克服了这些限制。 简言之，生物样品中的所有细胞都被荧光标记，但只有靶细胞也被磁性标记。 将标记的样品置于室或铜管中，放置在两个楔形磁体之间，以选择性地将磁性标记的细胞移动到铜管的观察表面。 LED照亮细胞，CCD相机捕获由靶细胞发射的荧光的图像。 用新算法进行的图像分析提供了与原始样品的靶细胞浓度相关的表面上的细胞计数。 紧凑型细胞仪系统提供坚固，经济实惠且易于使用的技术，可用于偏远地区。

公开(公告)号：US07282180B2

公开(公告)日：2007-10-16

申请号：US10612144

申请日：2003-07-02

申请人： Arjan Tibbe ,  Jan Greve ,  Leon W. M. M. Terstappen ,  Jan Greve, legal representative

发明人： Bart De Grooth, deceased

IPC分类号： G01N21/00

CPC分类号： G01N33/54326

摘要： Devices and methods for automated collection and image analysis are disclosed that enable identification or classification of microscopic objects aligned or deposited on surfaces. Such objects, e.g. detectably labeled rare target cells, are magnetically or non-magnetically immobilized and subjected to automated laser scanning to generate sequential digitized x-y sub-images or partial images of target and non-target objects that are combined to form reconstructed full images, thereby allowing detection, enumeration, differentiation and characterization of imaged objects on the basis of size, morphology and immunophenotype.

摘要翻译： 公开了用于自动收集和图像分析的装置和方法，其能够识别或分类对准或沉积在表面上的微观物体。 这些物体，例如。 可检测标记的稀有目标细胞是磁性或非磁性固定的，并进行自动激光扫描以产生被组合以形成重建的全部图像的目标和非目标对象的顺序数字化的xy子图像或部分图像，从而允许检测， 基于大小，形态和免疫表型的成像物体的计数，分化和表征。

公开(公告)号：US07943397B2

公开(公告)日：2011-05-17

申请号：US10903798

申请日：2004-07-30

申请人： Arjan Tibbe ,  Jan Greve ,  Dhanesh Gohel ,  Erik Droog ,  Leon W. M. M. Terstappen

发明人： Arjan Tibbe ,  Jan Greve ,  Dhanesh Gohel ,  Erik Droog ,  Leon W. M. M. Terstappen

IPC分类号： G01N33/00 ,  G01N33/553

CPC分类号： G01N15/1475 ,  G01N15/1463 ,  G01N33/5094 ,  G01N33/54333 ,  G01N2015/008 ,  G01N2015/1486 ,  Y10T436/101666 ,  Y10T436/25375

摘要： The enumeration of cells in fluids by flow cytometry is widely used across many disciplines such as assessment of leukocyte subsets in different bodily fluids or of bacterial contamination in environmental samples, food products and bodily fluids. For many applications the cost, size and complexity of the instruments prevents wider use, for example, CD4 analysis in HIV monitoring in resource-poor countries. The novel device, methods and algorithms disclosed herein largely overcome these limitations. Briefly, all cells in a biological sample are fluorescently labeled, but only the target cells are also magnetically labeled. The labeled sample, in a chamber or cuvet, is placed between two wedge-shaped magnets to selectively move the magnetically labeled cells to the observation surface of the cuvet. An LED illuminates the cells and a CCD camera captures the images of the fluorescent light emitted by the target cells. Image analysis performed with a novel algorithm provides a count of the cells on the surface that can be related to the target cell concentration of the original sample. The compact cytometer system provides a rugged, affordable and easy-to-use technique, which can be used in remote locations.

摘要翻译： 通过流式细胞术对流体细胞的计数被广泛应用于许多学科，例如评估不同体液中的白细胞亚群或环境样品，食品和体液中的细菌污染。 对于许多应用，仪器的成本，尺寸和复杂性阻止了更广泛的使用，例如资源贫乏国家的艾滋病毒监测中的CD4分析。 本文公开的新颖的装置，方法和算法在很大程度上克服了这些限制。 简言之，生物样品中的所有细胞都被荧光标记，但只有靶细胞也被磁性标记。 将标记的样品置于室或铜管中，放置在两个楔形磁体之间，以选择性地将磁性标记的细胞移动到铜管的观察表面。 LED照亮细胞，CCD相机捕获由靶细胞发射的荧光的图像。 用新算法进行的图像分析提供了与原始样品的靶细胞浓度相关的表面上的细胞计数。 紧凑型细胞仪系统提供坚固，经济实惠且易于使用的技术，可用于偏远地区。

公开(公告)号：US20060147901A1

公开(公告)日：2006-07-06

申请号：US11344757

申请日：2006-02-01

申请人： Greve Jan ,  Federik Schreuder ,  Leon Terstappen ,  Arjan Tibbe

发明人： Greve Jan ,  Federik Schreuder ,  Leon Terstappen ,  Arjan Tibbe

IPC分类号： C12Q1/00 ,  G06K9/00

CPC分类号： G01N33/54326

摘要： Devices and methods for automated collection and image analysis are disclosed that enable identification or classification of microscopic objects aligned or deposited on surfaces. Such objects, e.g. detectably labeled rare target cells, are magnetically or non-magnetically immobilized and subjected to Time Delay Integration imaging (TDI). Incorporation of TDI technology into image cytometry analysis, like CellTracks®, makes it possible to image moving objects with very high sensitivity and signal-to-noise ratios. Implementation of TDI camera technology with dual excitation and multispectral imaging of enriched rare cells provides a rapid system for detection, enumeration, differentiation and characterization of imaged rare cells on the basis of size, morphology and immunophenotype.

摘要翻译： 公开了用于自动收集和图像分析的装置和方法，其能够识别或分类对准或沉积在表面上的微观物体。 这些物体，例如。 可检测标记的罕见靶细胞是磁性或非磁性固定的并经受时间延迟积分成像（TDI）。 将TDI技术结合到图像细胞分析中，像CellTracks（R），使得可以以非常高的灵敏度和信噪比来对移动物体进行成像。 基于大小，形态和免疫表型，实现了具有双重激发和多光谱成像的富集稀有细胞的TDI相机技术，提供了快速检测，计数，分化和表征成像稀有细胞的系统。

公开(公告)号：US20050003464A1

公开(公告)日：2005-01-06

申请号：US10612144

申请日：2003-07-02

申请人： Arjan Tibbe ,  Jan Greve ,  Leon Terstappen ,  Bart De Grooth

发明人： Arjan Tibbe ,  Jan Greve ,  Leon Terstappen ,  Bart De Grooth

IPC分类号： C12M1/34 ,  G01N33/543 ,  G01N33/574

CPC分类号： G01N33/54326

摘要： Devices and methods for automated collection and image analysis are disclosed that enable identification or classification of microscopic objects aligned or deposited on surfaces. Such objects, e.g. detectably labeled rare target cells, are magnetically or non-magnetically immobilized and subjected to automated laser scanning to generate sequential digitized x-y sub-images or partial images of target and non-target objects that are combined to form reconstructed full images, thereby allowing detection, enumeration, differentiation and characterization of imaged objects on the basis of size, morphology and immunophenotype.

摘要翻译： 公开了用于自动收集和图像分析的装置和方法，其能够识别或分类对准或沉积在表面上的微观物体。 这些物体，例如。 可检测标记的稀有目标细胞是磁性或非磁性固定的，并进行自动激光扫描以产生被组合以形成重建的全部图像的目标和非目标对象的顺序数字化的xy子图像或部分图像，从而允许检测， 基于大小，形态和免疫表型的成像物体的计数，分化和表征。