-
公开(公告)号:US07255705B2
公开(公告)日:2007-08-14
申请号:US10743707
申请日:2003-12-24
申请人: Lih Tao Hsu , Wannhsin Chen , Kuang Ning Chang , Mei Ju Yang , Jinn Fa Wu , Yeou Bin Guu
发明人: Lih Tao Hsu , Wannhsin Chen , Kuang Ning Chang , Mei Ju Yang , Jinn Fa Wu , Yeou Bin Guu
IPC分类号: A62B17/3213 , B26B21/00
CPC分类号: B26B5/008 , A61B17/32 , A61B17/32093 , A61B17/3211
摘要: A device for dissecting an object, such as a cell colony includes a handle and an incision knife module that can dissociate the colony into several colony pieces. In accordance with features of the invention, users can use this dissecting device to cut an object into several pieces quickly and smoothly at a time.
摘要翻译: 用于解剖物体(例如细胞集落)的装置包括手柄和切割刀模块,其可以将菌落分解成几个菌落片。 根据本发明的特征,用户可以使用该解剖装置一次将物体快速平稳地切割成几件。
-
公开(公告)号:US20060030040A1
公开(公告)日:2006-02-09
申请号:US11233055
申请日:2005-09-23
申请人: Mei-Ju Yang , Kuang-Ning Chang , Lih-Tao Hsu , Jun-Jae Huang , Wann-Hsin Chen , Chao-Ying Kuo
发明人: Mei-Ju Yang , Kuang-Ning Chang , Lih-Tao Hsu , Jun-Jae Huang , Wann-Hsin Chen , Chao-Ying Kuo
CPC分类号: C12N5/0606 , C12N2500/84 , C12N2502/13 , C12N2533/90
摘要: Provided herein are human embryonic stem cells and their method of culturing. In one embodiment, the human embryonic stem cells are maintained in an environment containing an extracellular matrix isolated from feeder cells and a conditioned medium pre-conditioned by the feeder cells; the feeder cells are pre-inactivated by either gamma ray radiation or by mitomycin C treatment. The cultured human embryonic stem cells remain substantially undifferentiated and maintain their pluripotency to differentiate into three germ layer cells.
摘要翻译: 本文提供人胚胎干细胞及其培养方法。 在一个实施方案中,将人胚胎干细胞保持在含有从饲养细胞分离的细胞外基质和由饲养细胞预处理的条件培养基的环境中; 饲养细胞通过γ射线辐射或丝裂霉素C处理预灭活。 培养的人胚胎干细胞保持基本上未分化,并保持其多能性以分化成三个胚层细胞。
-