Human embryonic stem cells and culturing methods thereof
    12.
    发明申请
    Human embryonic stem cells and culturing methods thereof 失效
    人胚胎干细胞及其培养方法

    公开(公告)号:US20060030040A1

    公开(公告)日:2006-02-09

    申请号:US11233055

    申请日:2005-09-23

    IPC分类号: C12N5/08 C12N5/06

    摘要: Provided herein are human embryonic stem cells and their method of culturing. In one embodiment, the human embryonic stem cells are maintained in an environment containing an extracellular matrix isolated from feeder cells and a conditioned medium pre-conditioned by the feeder cells; the feeder cells are pre-inactivated by either gamma ray radiation or by mitomycin C treatment. The cultured human embryonic stem cells remain substantially undifferentiated and maintain their pluripotency to differentiate into three germ layer cells.

    摘要翻译: 本文提供人胚胎干细胞及其培养方法。 在一个实施方案中,将人胚胎干细胞保持在含有从饲养细胞分离的细胞外基质和由饲养细胞预处理的条件培养基的环境中; 饲养细胞通过γ射线辐射或丝裂霉素C处理预灭活。 培养的人胚胎干细胞保持基本上未分化,并保持其多能性以分化成三个胚层细胞。