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公开(公告)号：US20060030040A1
公开(公告)日：2006-02-09
申请号：US11233055
申请日：2005-09-23
申请人： Mei-Ju Yang , Kuang-Ning Chang , Lih-Tao Hsu , Jun-Jae Huang , Wann-Hsin Chen , Chao-Ying Kuo
发明人： Mei-Ju Yang , Kuang-Ning Chang , Lih-Tao Hsu , Jun-Jae Huang , Wann-Hsin Chen , Chao-Ying Kuo
CPC分类号： C12N5/0606 , C12N2500/84 , C12N2502/13 , C12N2533/90
摘要： Provided herein are human embryonic stem cells and their method of culturing. In one embodiment, the human embryonic stem cells are maintained in an environment containing an extracellular matrix isolated from feeder cells and a conditioned medium pre-conditioned by the feeder cells; the feeder cells are pre-inactivated by either gamma ray radiation or by mitomycin C treatment. The cultured human embryonic stem cells remain substantially undifferentiated and maintain their pluripotency to differentiate into three germ layer cells.
摘要翻译： 本文提供人胚胎干细胞及其培养方法。 在一个实施方案中，将人胚胎干细胞保持在含有从饲养细胞分离的细胞外基质和由饲养细胞预处理的条件培养基的环境中; 饲养细胞通过γ射线辐射或丝裂霉素C处理预灭活。 培养的人胚胎干细胞保持基本上未分化，并保持其多能性以分化成三个胚层细胞。
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2.发明申请Culture system and method for maintenance and proliferation of undifferentiated human embryonic stem cells 审中-公开未分化人胚胎干细胞的维持和增殖的培养系统和方法公开(公告)号：US20050124063A1
公开(公告)日：2005-06-09
申请号：US10839212
申请日：2004-05-06
申请人： Mei-Ju Yang , Kuang-Ning Chang , Lih-Tao Hsu , Jun-Jae Huang , Wann-Hsin Chen , Chao-Ying Kuo
发明人： Mei-Ju Yang , Kuang-Ning Chang , Lih-Tao Hsu , Jun-Jae Huang , Wann-Hsin Chen , Chao-Ying Kuo
IPC分类号： C12M3/00 , C12N5/00 , C12N5/02 , C12N5/0735 , C12N5/08
CPC分类号： C12N5/0606 , C12N2500/84 , C12N2502/13 , C12N2533/90
摘要： The present invention discloses an improved culture system for proliferative and undifferentiated growth of human embryonic stem cells, comprising an extracellular matrix prepared from feeder cells and a conditioned medium preconditioned by feeder cells. This invention also relates to a culture method utilizing the foregoing culture system.
摘要翻译： 本发明公开了一种用于人胚胎干细胞增殖和未分化生长的改良培养系统，其包括由饲养细胞制备的细胞外基质和由饲养细胞预处理的条件培养基。 本发明还涉及利用上述培养系统的培养方法。
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公开(公告)号：US20060141616A1
公开(公告)日：2006-06-29
申请号：US11317701
申请日：2005-12-23
申请人： Yeou-Bin Guu , Yu-Hsi Hsing , Wann-Hsin Chen , Lih-Tao Hsu , Jinn-Fa Wu
发明人： Yeou-Bin Guu , Yu-Hsi Hsing , Wann-Hsin Chen , Lih-Tao Hsu , Jinn-Fa Wu
摘要： A cell colonies dissecting and transplanting apparatus comprises: a base, a platform, a feeding mechanism having at least three axis feeding means, a movable holder, a cutting means, a transplanting means, and a CCD image capturing means. The platform having the CCD image capturing means therebottom is disposed onto the base, and on the platform is defined with the holder for receiving a plurality of loading trays, so as to the original cell colonies and a plurality of media boards of subculture can be placed on the loading trays of holder, the holder can displace from a first axis feeding means and a second axis feeding means of feeding mechanism to moveably align with a first axis and a second axis. In addition, the cutting means and the transplanting means respectively capable of vertically move along a third axis feeding means of feeding mechanism are arranged onto the base, wherein the cutting means is provided with an array of invertable blades, and the transplanting means is defined with a bent capture needle for capturing the cell masses, whereby enabling to use operational analysis of controller to precisely cut, capture and pickedly place the original cell colonies.
摘要翻译： 细胞集落解剖和移植装置包括：基座，平台，具有至少三轴进给装置的进给机构，可动夹持器，切割装置，移植装置和CCD图像捕获装置。 将具有CCD图像捕获装置的平台设置在基座上，并且在平台上限定有用于容纳多个装载盘的保持器，以便可以放置原始细胞集落和多个传播介质板 在保持器的装载托盘上，保持器可以从第一轴进给装置和进给机构的第二轴进给装置移位以与第一轴和第二轴可移动地对准。 此外，切割装置和分别能够沿着进给机构的第三轴进给装置垂直移动的移植装置布置在基部上，其中切割装置设置有可逆转刀片阵列，并且移植装置被定义为 用于捕获细胞块的弯曲捕获针，由此能够使用控制器的操作分析来精确地切割，捕获和拾取并放置原始细胞集落。
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公开(公告)号：US20080148913A1
公开(公告)日：2008-06-26
申请号：US11693656
申请日：2007-03-29
申请人： Jan-Hao Chen , Yeou-Bin Guu , Yung-Feng Nien , Wann-Hsin Chen , Lih-Tao Hsu
发明人： Jan-Hao Chen , Yeou-Bin Guu , Yung-Feng Nien , Wann-Hsin Chen , Lih-Tao Hsu
IPC分类号： G01N1/06
CPC分类号： G01N1/06 , Y10T83/0448 , Y10T83/6668
摘要： A dissection device for organisms includes a table bearing an organism and at least one blade lifted or lowered to approach the table. The blade includes a connecting portion, a cutting portion having a curved edge, and a neck portion connecting the connecting portion and the cutting portion. When the blade is lowered to contact the table, the cutting portion is rotated with respect to the neck portion to have rolling contact with the table from a first point to a second point of the edge.
摘要翻译： 用于生物体的解剖装置包括具有生物体的桌子和至少一个升降的叶片以接近桌子。 刀片包括连接部分，具有弯曲边缘的切割部分和连接连接部分和切割部分的颈部。 当叶片降低以接触桌子时，切割部分相对于颈部部分旋转以与台面滚动接触，从边缘的第一点到第二点。
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公开(公告)号：US20080161722A1
公开(公告)日：2008-07-03
申请号：US11690064
申请日：2007-03-22
申请人： Yeou-Bin Guu , Jan-Hao Chen , Lih-Tao Hsu , Wann-Hsin Chen
发明人： Yeou-Bin Guu , Jan-Hao Chen , Lih-Tao Hsu , Wann-Hsin Chen
IPC分类号： A61B10/02
CPC分类号： A61B10/0283 , A61B2017/320008
摘要： An aspiration device for cells or tissues loaded on a container includes a tubular body, a scraper disposed on the tubular body. The scraper scrapes cells or tissues from the container, and the scraped cells or tissues are suctioned into the tubular body. The scraper includes a connecting portion connected to the tubular body, an elastic portion connected to the connecting portion, and a scraping end connected to the elastic portion. When the scraper scrapes the cells or the tissues, the elastic portion deforms elastically to enable the scraping end to abut the surface of the container, thus, the cells or tissues are scraped.
摘要翻译： 用于装载在容器上的细胞或组织的抽吸装置包括管状体，设置在管状体上的刮刀。 刮刀从容器中刮下细胞或组织，将刮下的细胞或组织吸入管状体。 刮刀包括连接到管状体的连接部分，连接到连接部分的弹性部分和连接到弹性部分的刮削端。 当刮板刮擦细胞或组织时，弹性部分弹性变形，使得刮削端能够抵靠容器的表面，从而刮除细胞或组织。
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公开(公告)号：US07727760B2
公开(公告)日：2010-06-01
申请号：US11317701
申请日：2005-12-23
申请人： Yeou-Bin Guu , Yu-Hsi Hsing , Wann-Hsin Chen , Lih-Tao Hsu , Jinn-Fa Wu
发明人： Yeou-Bin Guu , Yu-Hsi Hsing , Wann-Hsin Chen , Lih-Tao Hsu , Jinn-Fa Wu
摘要： A cell colonies dissecting and transplanting apparatus comprises: a base, a platform, a feeding mechanism having at least three axis feeding means, a movable holder, a cutting means, a transplanting means, and a CCD image capturing means. The platform having the CCD image capturing means therebottom is disposed onto the base, and on the platform is defined with the holder for receiving a plurality of loading trays, so as to the original cell colonies and a plurality of media boards of subculture can be placed on the loading trays of holder, the holder can displace from a first axis feeding means and a second axis feeding means of feeding mechanism to moveably align with a first axis and a second axis. In addition, the cutting means and the transplanting means respectively capable of vertically move along a third axis feeding means of feeding mechanism are arranged onto the base, wherein the cutting means is provided with an array of invertable blades, and the transplanting means is defined with a bent capture needle for capturing the cell masses, whereby enabling to use operational analysis of controller to precisely cut, capture and pickedly place the original cell colonies.
摘要翻译： 细胞集落解剖和移植装置包括：基座，平台，具有至少三轴进给装置的进给机构，可动夹持器，切割装置，移植装置和CCD图像捕获装置。 将具有CCD图像捕获装置的平台设置在基座上，并且在平台上限定有用于容纳多个装载盘的保持器，以便可以放置原始细胞集落和多个传播介质板 在保持器的装载托盘上，保持器可以从第一轴进给装置和进给机构的第二轴进给装置移位以与第一轴和第二轴可移动地对准。 此外，切割装置和分别能够沿着进给机构的第三轴进给装置垂直移动的移植装置布置在基部上，其中切割装置设置有可逆转刀片阵列，并且移植装置被定义为 用于捕获细胞块的弯曲捕获针，由此能够使用控制器的操作分析来精确地切割，捕获和拾取并放置原始细胞集落。
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公开(公告)号：US20070148767A1
公开(公告)日：2007-06-28
申请号：US11318882
申请日：2005-12-28
申请人： Mei-Ju Yang , Wann-Hsin Chen , Pei-Ju Lin , Chun-Hung Chen , Hsing-Wen Sung
发明人： Mei-Ju Yang , Wann-Hsin Chen , Pei-Ju Lin , Chun-Hung Chen , Hsing-Wen Sung
CPC分类号： C12N5/0068 , C12N5/0606 , C12N5/067 , C12N2533/78
摘要： Provided herein are methods for producing multicellular spheroids or embryoid bodies suitable for providing cells in large scale for various medical applications. In one embodiment, a method of forming embryoid bodies is provided, which comprises culturing undifferentiated HES cells in a culture vessel pre-coated with cellulose and/or its derivatives. In another embodiment, a method of forming hepatic spheroids is provided, which comprises culturing hepatocytes in a culture vessel pre-coated with cellulose and/or its derivatives.
摘要翻译： 本文提供了适用于大规模提供用于各种医疗应用的细胞的多细胞球体或胚状体的方法。 在一个实施方案中，提供了形成胚状体的方法，其包括在预先包被纤维素和/或其衍生物的培养容器中培养未分化的HES细胞。 在另一个实施方案中，提供了形成肝球体的方法，其包括在预先涂有纤维素和/或其衍生物的培养容器中培养肝细胞。
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公开(公告)号：US07534607B1
公开(公告)日：2009-05-19
申请号：US11319113
申请日：2005-12-27
申请人： Wann-Hsin Chen , Su-Yo Lin
发明人： Wann-Hsin Chen , Su-Yo Lin
CPC分类号： C12N5/0657 , C12N2500/38
摘要： Methods are provided for producing cardiomyocytes suitable for various medical applications. In one embodiment, a method of producing cardiomyocytes is provided, which comprises culturing mesenchymal stem cells in a medium containing ascorbic acid. In another embodiment, such method further comprises the step of selecting fast-growing mesenchymal stem cell clones before culturing the selected clones in the culturing medium that contains ascorbic acid.
摘要翻译： 提供用于产生适合于各种医疗应用的心肌细胞的方法。 在一个实施方案中，提供了产生心肌细胞的方法，其包括在含有抗坏血酸的培养基中培养间充质干细胞。 在另一个实施方案中，这种方法还包括在培养含有抗坏血酸的培养基中选择的克隆之前选择快速生长的间充质干细胞克隆的步骤。
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公开(公告)号：US07981630B2
公开(公告)日：2011-07-19
申请号：US12483434
申请日：2009-06-12
申请人： Chen-Ming Chen , Wann-Hsin Chen
发明人： Chen-Ming Chen , Wann-Hsin Chen
IPC分类号： G01N33/567 , C12N5/00
CPC分类号： G01N33/5073 , C12Q1/6881 , C12Q2600/158
摘要： Polynucleotides associated with differentiation states of stem cells are provided. Also provided are methods and kits for detecting, identifying and/or discriminating differentiated stem cells from undifferentiated ones by measuring an expression level of one or more genes, such as CBARA1 and LHX 6, in the stem cells.
摘要翻译： 提供了与干细胞分化状态相关的多核苷酸。 还提供了用于通过测量干细胞中一种或多种基因（例如CBARA1和LHX6）的表达水平来检测，鉴定和/或区分来自未分化干细胞的分化干细胞的方法和试剂盒。
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公开(公告)号：US20100316997A1
公开(公告)日：2010-12-16
申请号：US12483434
申请日：2009-06-12
申请人： Chen-Ming Chen , Wann-Hsin Chen
发明人： Chen-Ming Chen , Wann-Hsin Chen
CPC分类号： G01N33/5073 , C12Q1/6881 , C12Q2600/158
摘要： Polynucleotides associated with differentiation states of stem cells are provided. Also provided are methods and kits for detecting, identifying and/or discriminating differentiated stem cells from undifferentiated ones by measuring an expression level of one or more genes, such as CBARA1 and LHX 6, in the stem cells.
摘要翻译： 提供了与干细胞分化状态相关的多核苷酸。 还提供了用于通过测量干细胞中一种或多种基因（例如CBARA1和LHX6）的表达水平来检测，鉴定和/或区分来自未分化干细胞的分化干细胞的方法和试剂盒。
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