公开(公告)号：US07727760B2

公开(公告)日：2010-06-01

申请号：US11317701

申请日：2005-12-23

申请人： Yeou-Bin Guu ,  Yu-Hsi Hsing ,  Wann-Hsin Chen ,  Lih-Tao Hsu ,  Jinn-Fa Wu

发明人： Yeou-Bin Guu ,  Yu-Hsi Hsing ,  Wann-Hsin Chen ,  Lih-Tao Hsu ,  Jinn-Fa Wu

IPC分类号： C12M1/26 ,  C12M3/00 ,  G01N1/30 ,  G01N33/48

CPC分类号： C12M23/50 ,  C12M33/00 ,  G01N1/286

摘要： A cell colonies dissecting and transplanting apparatus comprises: a base, a platform, a feeding mechanism having at least three axis feeding means, a movable holder, a cutting means, a transplanting means, and a CCD image capturing means. The platform having the CCD image capturing means therebottom is disposed onto the base, and on the platform is defined with the holder for receiving a plurality of loading trays, so as to the original cell colonies and a plurality of media boards of subculture can be placed on the loading trays of holder, the holder can displace from a first axis feeding means and a second axis feeding means of feeding mechanism to moveably align with a first axis and a second axis. In addition, the cutting means and the transplanting means respectively capable of vertically move along a third axis feeding means of feeding mechanism are arranged onto the base, wherein the cutting means is provided with an array of invertable blades, and the transplanting means is defined with a bent capture needle for capturing the cell masses, whereby enabling to use operational analysis of controller to precisely cut, capture and pickedly place the original cell colonies.

摘要翻译： 细胞集落解剖和移植装置包括：基座，平台，具有至少三轴进给装置的进给机构，可动夹持器，切割装置，移植装置和CCD图像捕获装置。 将具有CCD图像捕获装置的平台设置在基座上，并且在平台上限定有用于容纳多个装载盘的保持器，以便可以放置原始细胞集落和多个传播介质板 在保持器的装载托盘上，保持器可以从第一轴进给装置和进给机构的第二轴进给装置移位以与第一轴和第二轴可移动地对准。 此外，切割装置和分别能够沿着进给机构的第三轴进给装置垂直移动的移植装置布置在基部上，其中切割装置设置有可逆转刀片阵列，并且移植装置被定义为 用于捕获细胞块的弯曲捕获针，由此能够使用控制器的操作分析来精确地切割，捕获和拾取并放置原始细胞集落。

公开(公告)号：US20050124063A1

公开(公告)日：2005-06-09

申请号：US10839212

申请日：2004-05-06

申请人： Mei-Ju Yang ,  Kuang-Ning Chang ,  Lih-Tao Hsu ,  Jun-Jae Huang ,  Wann-Hsin Chen ,  Chao-Ying Kuo

发明人： Mei-Ju Yang ,  Kuang-Ning Chang ,  Lih-Tao Hsu ,  Jun-Jae Huang ,  Wann-Hsin Chen ,  Chao-Ying Kuo

IPC分类号： C12M3/00 ,  C12N5/00 ,  C12N5/02 ,  C12N5/0735 ,  C12N5/08

CPC分类号： C12N5/0606 ,  C12N2500/84 ,  C12N2502/13 ,  C12N2533/90

摘要： The present invention discloses an improved culture system for proliferative and undifferentiated growth of human embryonic stem cells, comprising an extracellular matrix prepared from feeder cells and a conditioned medium preconditioned by feeder cells. This invention also relates to a culture method utilizing the foregoing culture system.

摘要翻译： 本发明公开了一种用于人胚胎干细胞增殖和未分化生长的改良培养系统，其包括由饲养细胞制备的细胞外基质和由饲养细胞预处理的条件培养基。 本发明还涉及利用上述培养系统的培养方法。

公开(公告)号：US20090162925A1

公开(公告)日：2009-06-25

申请号：US11965528

申请日：2007-12-27

申请人： Wannhsin Chen ,  Lih-Tao Hsu ,  Yeou-Bin Guu ,  Yu Mei Chen

发明人： Wannhsin Chen ,  Lih-Tao Hsu ,  Yeou-Bin Guu ,  Yu Mei Chen

IPC分类号： C12M1/00

CPC分类号： G01N1/06 ,  C12M33/04 ,  G01N1/2813 ,  Y10T83/263 ,  Y10T83/283

摘要： A selecting apparatus for selecting cell/tissue mass includes a base, a feeding mechanism and a dividing mechanism. The base has a platform for placing the cell/tissue mass. The feeding mechanism, disposed on the base, moves relative to the platform. The dividing mechanism, disposed on the feeding mechanism, includes a first cutting set and a second cutting set connected therewith. The second cutting set reciprocally moves between a first position and a second position, toward the first cutting set. The feeding mechanism moves the dividing mechanism above the platform, aligning the dividing mechanism with a portion of the cell/tissue mass, and moves the first cutting set toward the cell/tissue mass, cutting into the portion of the cell/tissue mass. The second cutting set moves from the first position to the second position, limiting the portion of the cell/tissue mass between the first cutting set and the second cutting set.

摘要翻译： 用于选择细胞/组织块的选择装置包括基底，进给机构和分割机构。 该基座具有放置细胞/组织块的平台。 设置在基座上的进给机构相对于平台移动。 配置在供给机构上的分割机构包括第一切割组和与其连接的第二切割组。 第二切割组在第一位置和第二位置之间往复移动朝向第一切割组。 供给机构将分割机构移动到平台上方，使分割机构与细胞/组织块的一部分对准，并使第一切割组朝向细胞/组织块移动，切割成细胞/组织块的部分。 第二切割组从第一位置移动到第二位置，限制第一切割组和第二切割组之间的细胞/组织块的部分。

公开(公告)号：US20060141616A1

公开(公告)日：2006-06-29

申请号：US11317701

申请日：2005-12-23

申请人： Yeou-Bin Guu ,  Yu-Hsi Hsing ,  Wann-Hsin Chen ,  Lih-Tao Hsu ,  Jinn-Fa Wu

发明人： Yeou-Bin Guu ,  Yu-Hsi Hsing ,  Wann-Hsin Chen ,  Lih-Tao Hsu ,  Jinn-Fa Wu

IPC分类号： C12M1/34 ,  C12M1/36

CPC分类号： C12M23/50 ,  C12M33/00 ,  G01N1/286

摘要： A cell colonies dissecting and transplanting apparatus comprises: a base, a platform, a feeding mechanism having at least three axis feeding means, a movable holder, a cutting means, a transplanting means, and a CCD image capturing means. The platform having the CCD image capturing means therebottom is disposed onto the base, and on the platform is defined with the holder for receiving a plurality of loading trays, so as to the original cell colonies and a plurality of media boards of subculture can be placed on the loading trays of holder, the holder can displace from a first axis feeding means and a second axis feeding means of feeding mechanism to moveably align with a first axis and a second axis. In addition, the cutting means and the transplanting means respectively capable of vertically move along a third axis feeding means of feeding mechanism are arranged onto the base, wherein the cutting means is provided with an array of invertable blades, and the transplanting means is defined with a bent capture needle for capturing the cell masses, whereby enabling to use operational analysis of controller to precisely cut, capture and pickedly place the original cell colonies.

摘要翻译： 细胞集落解剖和移植装置包括：基座，平台，具有至少三轴进给装置的进给机构，可动夹持器，切割装置，移植装置和CCD图像捕获装置。 将具有CCD图像捕获装置的平台设置在基座上，并且在平台上限定有用于容纳多个装载盘的保持器，以便可以放置原始细胞集落和多个传播介质板 在保持器的装载托盘上，保持器可以从第一轴进给装置和进给机构的第二轴进给装置移位以与第一轴和第二轴可移动地对准。 此外，切割装置和分别能够沿着进给机构的第三轴进给装置垂直移动的移植装置布置在基部上，其中切割装置设置有可逆转刀片阵列，并且移植装置被定义为 用于捕获细胞块的弯曲捕获针，由此能够使用控制器的操作分析来精确地切割，捕获和拾取并放置原始细胞集落。

公开(公告)号：US08062593B2

公开(公告)日：2011-11-22

申请号：US12825136

申请日：2010-06-28

申请人： Wannhsin Chen ,  Lih-Tao Hsu ,  Yeou-Bin Guu ,  Yu Mei Chen

发明人： Wannhsin Chen ,  Lih-Tao Hsu ,  Yeou-Bin Guu ,  Yu Mei Chen

IPC分类号： G01N21/00

CPC分类号： G01N1/06 ,  C12M33/04 ,  G01N1/2813 ,  Y10T83/263 ,  Y10T83/283

摘要： A selecting apparatus for selecting cell/tissue mass includes a base, a feeding mechanism and a dividing mechanism. The base has a platform for placing the cell/tissue mass. The feeding mechanism, disposed on the base, moves relative to the platform. The dividing mechanism, disposed on the feeding mechanism, includes a first cutting set and a second cutting set connected therewith. The second cutting set reciprocally moves between a first position and a second position, toward the first cutting set. The feeding mechanism moves the dividing mechanism above the platform, aligning the dividing mechanism with a portion of the cell/tissue mass, and moves the first cutting set toward the cell/tissue mass, cutting into the portion of the cell/tissue mass. The second cutting set moves from the first position to the second position, limiting the portion of the cell/tissue mass between the first cutting set and the second cutting set.

摘要翻译： 用于选择细胞/组织块的选择装置包括基底，进给机构和分割机构。 该基座具有放置细胞/组织块的平台。 设置在基座上的进给机构相对于平台移动。 配置在供给机构上的分割机构包括第一切割组和与其连接的第二切割组。 第二切割组在第一位置和第二位置之间往复移动朝向第一切割组。 供给机构将分割机构移动到平台上方，使分割机构与细胞/组织块的一部分对准，并使第一切割组朝向细胞/组织块移动，切割成细胞/组织块的部分。 第二切割组从第一位置移动到第二位置，限制第一切割组和第二切割组之间的细胞/组织块的部分。

公开(公告)号：US20100261261A1

公开(公告)日：2010-10-14

申请号：US12825136

申请日：2010-06-28

申请人： Wannhsin Chen ,  Lih-Tao Hsu ,  Yeou-Bin Guu ,  Yu Mei Chen

发明人： Wannhsin Chen ,  Lih-Tao Hsu ,  Yeou-Bin Guu ,  Yu Mei Chen

IPC分类号： C12M1/00

CPC分类号： G01N1/06 ,  C12M33/04 ,  G01N1/2813 ,  Y10T83/263 ,  Y10T83/283

摘要： A selecting apparatus for selecting cell/tissue mass includes a base, a feeding mechanism and a dividing mechanism. The base has a platform for placing the cell/tissue mass. The feeding mechanism, disposed on the base, moves relative to the platform. The dividing mechanism, disposed on the feeding mechanism, includes a first cutting set and a second cutting set connected therewith. The second cutting set reciprocally moves between a first position and a second position, toward the first cutting set. The feeding mechanism moves the dividing mechanism above the platform, aligning the dividing mechanism with a portion of the cell/tissue mass, and moves the first cutting set toward the cell/tissue mass, cutting into the portion of the cell/tissue mass. The second cutting set moves from the first position to the second position, limiting the portion of the cell/tissue mass between the first cutting set and the second cutting set.

摘要翻译： 用于选择细胞/组织块的选择装置包括基底，进给机构和分割机构。 该基座具有放置细胞/组织块的平台。 设置在基座上的进给机构相对于平台移动。 配置在供给机构上的分割机构包括第一切割组和与其连接的第二切割组。 第二切割组在第一位置和第二位置之间往复移动朝向第一切割组。 供给机构将分割机构移动到平台上方，使分割机构与细胞/组织块的一部分对准，并使第一切割组朝向细胞/组织块移动，切割成细胞/组织块的部分。 第二切割组从第一位置移动到第二位置，限制第一切割组和第二切割组之间的细胞/组织块的部分。

公开(公告)号：US07842236B2

公开(公告)日：2010-11-30

申请号：US11965528

申请日：2007-12-27

申请人： Wannhsin Chen ,  Lih-Tao Hsu ,  Yeou-Bin Guu ,  Yu Mei Chen

发明人： Wannhsin Chen ,  Lih-Tao Hsu ,  Yeou-Bin Guu ,  Yu Mei Chen

IPC分类号： G01N21/00

CPC分类号： G01N1/06 ,  C12M33/04 ,  G01N1/2813 ,  Y10T83/263 ,  Y10T83/283

摘要： A selecting apparatus for selecting cell/tissue mass includes a base, a feeding mechanism and a dividing mechanism. The base has a platform for placing the cell/tissue mass. The feeding mechanism, disposed on the base, moves relative to the platform. The dividing mechanism, disposed on the feeding mechanism, includes a first cutting set and a second cutting set connected therewith. The second cutting set reciprocally moves between a first position and a second position, toward the first cutting set. The feeding mechanism moves the dividing mechanism above the platform, aligning the dividing mechanism with a portion of the cell/tissue mass, and moves the first cutting set toward the cell/tissue mass, cutting into the portion of the cell/tissue mass. The second cutting set moves from the first position to the second position, limiting the portion of the cell/tissue mass between the first cutting set and the second cutting set.

摘要翻译： 用于选择细胞/组织块的选择装置包括基底，进给机构和分割机构。 该基座具有放置细胞/组织块的平台。 设置在基座上的进给机构相对于平台移动。 配置在供给机构上的分割机构包括第一切割组和与其连接的第二切割组。 第二切割组在第一位置和第二位置之间往复移动朝向第一切割组。 供给机构将分割机构移动到平台上方，使分割机构与细胞/组织块的一部分对准，并使第一切割组朝向细胞/组织块移动，切割成细胞/组织块的部分。 第二切割组从第一位置移动到第二位置，限制第一切割组和第二切割组之间的细胞/组织块的部分。

公开(公告)号：US20080148913A1

公开(公告)日：2008-06-26

申请号：US11693656

申请日：2007-03-29

申请人： Jan-Hao Chen ,  Yeou-Bin Guu ,  Yung-Feng Nien ,  Wann-Hsin Chen ,  Lih-Tao Hsu

发明人： Jan-Hao Chen ,  Yeou-Bin Guu ,  Yung-Feng Nien ,  Wann-Hsin Chen ,  Lih-Tao Hsu

IPC分类号： G01N1/06

CPC分类号： G01N1/06 ,  Y10T83/0448 ,  Y10T83/6668

摘要： A dissection device for organisms includes a table bearing an organism and at least one blade lifted or lowered to approach the table. The blade includes a connecting portion, a cutting portion having a curved edge, and a neck portion connecting the connecting portion and the cutting portion. When the blade is lowered to contact the table, the cutting portion is rotated with respect to the neck portion to have rolling contact with the table from a first point to a second point of the edge.

摘要翻译： 用于生物体的解剖装置包括具有生物体的桌子和至少一个升降的叶片以接近桌子。 刀片包括连接部分，具有弯曲边缘的切割部分和连接连接部分和切割部分的颈部。 当叶片降低以接触桌子时，切割部分相对于颈部部分旋转以与台面滚动接触，从边缘的第一点到第二点。

公开(公告)号：US07682826B2

公开(公告)日：2010-03-23

申请号：US11233055

申请日：2005-09-23

申请人： Mei Ju Yang ,  Kuang Ning Chang ,  Lih Tao Hsu ,  Jun Jae Huang ,  Wann Hsin Chen ,  Chao Ying Kuo

发明人： Mei Ju Yang ,  Kuang Ning Chang ,  Lih Tao Hsu ,  Jun Jae Huang ,  Wann Hsin Chen ,  Chao Ying Kuo

IPC分类号： C12N5/00

CPC分类号： C12N5/0606 ,  C12N2500/84 ,  C12N2502/13 ,  C12N2533/90

摘要： Provided herein are human embryonic stem cells and their method of culturing. In one embodiment, the human embryonic stem cells are maintained in an environment containing an extracellular matrix isolated from feeder cells and a conditioned medium pre-conditioned by the feeder cells; the feeder cells are pre-inactivated by either gamma ray radiation or by mitomycin C treatment. The cultured human embryonic stem cells remain substantially undifferentiated and maintain their pluripotency to differentiate into three germ layer cells.

摘要翻译： 本文提供人胚胎干细胞及其培养方法。 在一个实施方案中，将人胚胎干细胞保持在含有从饲养细胞分离的细胞外基质和由饲养细胞预处理的条件培养基的环境中; 饲养细胞通过γ射线辐射或丝裂霉素C处理预灭活。 培养的人胚胎干细胞保持基本上未分化，并保持其多能性以分化成三个胚层细胞。

公开(公告)号：US20080161722A1

公开(公告)日：2008-07-03

申请号：US11690064

申请日：2007-03-22

申请人： Yeou-Bin Guu ,  Jan-Hao Chen ,  Lih-Tao Hsu ,  Wann-Hsin Chen

发明人： Yeou-Bin Guu ,  Jan-Hao Chen ,  Lih-Tao Hsu ,  Wann-Hsin Chen

IPC分类号： A61B10/02

CPC分类号： A61B10/0283 ,  A61B2017/320008

摘要： An aspiration device for cells or tissues loaded on a container includes a tubular body, a scraper disposed on the tubular body. The scraper scrapes cells or tissues from the container, and the scraped cells or tissues are suctioned into the tubular body. The scraper includes a connecting portion connected to the tubular body, an elastic portion connected to the connecting portion, and a scraping end connected to the elastic portion. When the scraper scrapes the cells or the tissues, the elastic portion deforms elastically to enable the scraping end to abut the surface of the container, thus, the cells or tissues are scraped.

摘要翻译： 用于装载在容器上的细胞或组织的抽吸装置包括管状体，设置在管状体上的刮刀。 刮刀从容器中刮下细胞或组织，将刮下的细胞或组织吸入管状体。 刮刀包括连接到管状体的连接部分，连接到连接部分的弹性部分和连接到弹性部分的刮削端。 当刮板刮擦细胞或组织时，弹性部分弹性变形，使得刮削端能够抵靠容器的表面，从而刮除细胞或组织。