公开(公告)号：US5202230A

公开(公告)日：1993-04-13

申请号：US912028

申请日：1992-07-09

申请人： Louis A. Kamentsky

发明人： Louis A. Kamentsky

IPC分类号： C12Q1/68 ,  G01N33/50

CPC分类号： G01N33/5005 ,  C12Q1/6841 ,  C12Q1/6886

摘要： A method of identifying a sectioned cell in a tissue section including: supplying a tissue section; labeling cells of the tissue section with a detectable label; and selectively detecting the label from cells at the surface of the tissue section.

摘要翻译： 一种识别组织切片中的切片细胞的方法，包括：提供组织切片; 用可检测标记标记组织切片的细胞; 并从组织切片表面的细胞中选择性地检测标记物。

公开(公告)号：US4487839A

公开(公告)日：1984-12-11

申请号：US455765

申请日：1983-01-05

申请人： Louis A. Kamentsky

发明人： Louis A. Kamentsky

IPC分类号： C12Q1/04 ,  G01N21/86 ,  G01N33/543 ,  G01N33/569 ,  G01N33/80 ,  G01N33/52 ,  G01N33/58

CPC分类号： G01N21/8483 ,  G01N33/543 ,  G01N33/54366 ,  G01N33/56966 ,  G01N33/80 ,  Y10S436/80 ,  Y10S436/804 ,  Y10S436/807 ,  Y10S436/808 ,  Y10S436/809 ,  Y10S436/823

摘要： Methods for determining the presence of antigens or antibodies in an aqueous sample or presence of antigens on the surface of cells. A preferred embodiment employs fluorescent antigens which compete with the sample antigens for antibody binding sites. The antibodies are deposited on a support surface means in alternating patterns. The surface means and fluorescence detector are translocated with respect to each other and a signal generated by the detection of the repeating pattern of fluorescence. The signal is analyzed by means of a gated integrator responsive to a gate track control means also located on the surface means. Immunoassay methods having increased sensitivity are thereby obtained.

摘要翻译： 用于测定水样品中抗原或抗体存在或细胞表面上存在抗原的方法。 优选的实施方案使用与样品抗原竞争抗体结合位点的荧光抗原。 抗体以交替的模式沉积在支撑表面上。 表面装置和荧光检测器相对于彼此移位，并且通过检测重复荧光图案产生的信号。 通过门控积分器来响应于也位于表面装置上的门轨道控制装置来分析信号。 由此获得具有增加的灵敏度的免疫测定方法。