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公开(公告)号：US20080199953A1
公开(公告)日：2008-08-21
申请号：US10602789
申请日：2003-06-24
CPC分类号： A61L27/3604 , A61K35/12 , A61L27/3804 , A61L27/386 , A61L27/3895 , C12N5/066 , C12N2500/38 , C12N2533/18 , C12N2533/52 , C12N2533/56
摘要： A system and method are provided for forming a connective tissue construct, such as a tendon construct, in vitro. A substrate is provided with at least two anchors secured thereto in spaced relationship. Fibroblast cells are provided on the substrate in the absence of a synthetic matrix, where at least some of the cells are in contact with the anchors. The cells are cultured in vitro under conditions to allow the cells to self-organize and become confluent between the anchors, where the anchors are receptive to the cells and allow the cells to attach thereto while permitting the cells to detach from the substrate to form a three-dimensional connective tissue construct.
摘要翻译： 提供了用于在体外形成结缔组织构建体（例如腱结构）的系统和方法。 基板具有至少两个以其间隔的关系固定到其上的锚固件。 在不存在合成基质的情况下，在基质上提供成纤维细胞，其中至少一些细胞与锚接触。 在体外培养细胞以允许细胞自我组织并在锚定体之间融合，其中锚固体接受细胞并允许细胞附着于细胞，同时允许细胞从基底分离以形成 三维结缔组织结构。
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公开(公告)号：US06448076B2
公开(公告)日：2002-09-10
申请号：US09896651
申请日：2001-06-29
IPC分类号： C12N500
CPC分类号： C12N5/0658 , A61K35/12 , C12N2533/52 , C12N2533/92
摘要： A method for chemically acellularizing a biological tissue sample, such as a peripheral nerve, is provided. The method includes disrupting the cell membranes of the biological tissue sample, and then denaturing intracellular proteins within the cells of the tissue sample and removing the denatured proteins from the cells while preserving the extracellular matrix to produce an acellularized tissue construct.
摘要翻译： 提供了一种对生物组织样品如周围神经进行化学脱细胞化的方法。 该方法包括破坏生物组织样品的细胞膜，然后使组织样品的细胞内的细胞内蛋白质变性并从细胞中除去变性的蛋白质，同时保留细胞外基质以产生无细胞化的组织构建体。
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公开(公告)号：US06207451B1
公开(公告)日：2001-03-27
申请号：US09153721
申请日：1998-09-15
IPC分类号： C12N500
CPC分类号： C12N5/0658 , A61K35/12 , C12N2533/52
摘要： A mammalian muscle construct and a method for producing the construct are provided. The mammalian muscle construct includes a substrate and a plurality of separate anchors secured to the substrate. Myogenic precursor cells are provided on the substrate with at least some of the cells in contact with the anchors. The myogenic precursor cells are cultured in vitro under conditions to allow the cells to become confluent between the anchors. The anchors are receptive to the cells and allow the cells to attach thereto, such that placement of the anchors controls the size and shape of the muscle construct formed. Specifically, the anchors include separate fragments of biocompatible material secured to the substrate, wherein cell adhesion molecules are associated with each fragment to facilitate attachment of the precursor cells to the fragment.
摘要翻译： 提供哺乳动物肌肉构造物和制备该构建体的方法。 哺乳动物肌肉构造物包括基底和固定到基底的多个单独的锚。 在底物上提供生肌前体细胞，其中至少一些细胞与锚固体接触。 在体外培养肌原性前体细胞的条件下使细胞在锚定体之间变得融合。 锚固体接受细胞并允许细胞附着于其上，使得锚的放置控制形成的肌肉构造的大小和形状。 具体地，锚固体包括固定到底物的生物相容性材料的分开的片段，其中细胞粘附分子与每个片段相关联，以促进前体细胞与片段的连接。
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14.发明授权System and method for emulating an in vivo environment of a muscle tissue specimen 失效用于模拟肌肉组织标本的体内环境的系统和方法
公开(公告)号：US06114164A
公开(公告)日：2000-09-05
申请号：US206588
申请日：1998-12-07
申请人： Robert G. Dennis , Paul Kosnik
发明人： Robert G. Dennis , Paul Kosnik
IPC分类号： C12M1/36
摘要： A system and method are provided for adaptively controlling a muscle tissue specimen in order to emulate its in vivo environment. The system includes at least one stimulator for stimulating the muscle tissue specimen based on an initial control signal, wherein the stimulation preferably includes electrical and/or mechanical stimulation. A response signal is generated based on a response of the muscle tissue specimen to the step of stimulating. The response signal preferably represents force production of the tissue specimen. A controller is provided for modifying the initial control signal based on the response signal to obtain a final control signal, wherein the final control signal is used to elicit a desired response from the muscle tissue specimen. Advantageously, the system and method of the present invention can be used to adaptively control the stimulation of a muscle tissue specimen in a tissue culture environment.
摘要翻译： 提供了一种用于自适应地控制肌肉组织样本以便模拟其体内环境的系统和方法。 该系统包括至少一个用于基于初始控制信号刺激肌肉组织样本的刺激器，其中所述刺激优选地包括电和/或机械刺激。 基于肌肉组织样本对刺激步骤的响应产生响应信号。 响应信号优选地表示组织标本的力产生。 提供控制器，用于基于响应信号修改初始控制信号以获得最终控制信号，其中最终控制信号用于从肌肉组织标本引出期望的响应。 有利地，本发明的系统和方法可用于适应性地控制组织培养环境中肌肉组织标本的刺激。
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公开(公告)号：US5555470A
公开(公告)日：1996-09-10
申请号：US386120
申请日：1995-02-09
申请人： Neil M. Cole , Robert G. Dennis
发明人： Neil M. Cole , Robert G. Dennis
摘要： A Michelson interferometer is used to determine the displacement of a force loaded member, the loaded member having a reflective surface applied thereto to reflect light back to a beam splitter in the interferometer apparatus. Light is also reflected back from a reflective surface attached to a stationary member. The interaction of the reflected light from the movable and stationary member interacts to form an interference fringe pattern which may be used to determine the amount of displacement and, therefor, the force applied to the loaded member. The apparatus is comprised of a material which is substantially resistive to thermal deformation.
摘要翻译： 迈克尔逊干涉仪用于确定受力部件的位移，加载部件具有施加到其上的反射表面，以将光反射回干涉仪装置中的分束器。 光也从附接到固定构件的反射表面反射回来。 来自可移动和固定构件的反射光的相互作用相互作用以形成干涉条纹图案，该干涉条纹图案可用于确定位移量，并因此确定施加到加载构件的力。 该装置由基本上抵抗热变形的材料构成。
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