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公开(公告)号：US08202725B2
公开(公告)日：2012-06-19
申请号：US11314281
申请日：2005-12-22
IPC分类号： C12N5/00
CPC分类号： C12N5/0697 , C12M21/08 , C12M25/10 , C12M25/14 , C12M27/10 , C12M29/10 , C12N2533/30
摘要： Tissue engineering methods and biochamber apparatus are provided for making tissue grafts for implantation into a patient. The methods include applying a sustained low magnitude pressure gradient transmurally across a permeable scaffold material using a media containing cells, preferably microvascular epithelial cells, to be deposited on the scaffold for the production of tissue grafts, preferably vascular grafts, to promote accelerated adhesion and maturation of cells on the scaffold material. Biochambers for preparing tubular tissue grafts are provided which contain connectors for holding a graft substrate, proximal and distal tubing for connection to an optional perfusion system, and structure for switching between transmural flow of a cell suspension across the graft substrate and translumenal flow through the lumen of the graft.
摘要翻译： 提供组织工程方法和生物处理设备用于制造植入患者体内的组织移植物。 所述方法包括使用包含细胞（优选微血管上皮细胞）的培养基透过可渗透的支架材料跨度施加持续的低幅度压力梯度，以沉积在支架上用于生产组织移植物，优选血管移植物，以促进加速粘附和成熟 的细胞在脚手架材料上。 提供了用于制备管状组织移植物的生物体，其包含用于保持移植物基质的连接器，用于连接到任选的灌注系统的近端和远端管，以及用于在穿过移植物基质的细胞悬浮液的跨壁流和通过内腔的经血流之间切换的结构 的移植物。
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公开(公告)号：US07575759B2
公开(公告)日：2009-08-18
申请号：US10330578
申请日：2002-12-27
IPC分类号： A61F2/00
摘要： The present invention relates to methods and compositions for the production of scaffolds, such scaffolds to be used for a variety of purposes, including tissue engineering. More specifically, the present invention relates to the use of fused crystals, such as fused salt crystals to form a framework. The methods for producing the scaffolds of this invention improve the porosity, interconnectivity and ease of manufacture as compared to prior art methods.
摘要翻译： 本发明涉及用于生产支架的方法和组合物，所述支架用于各种目的，包括组织工程。 更具体地，本发明涉及使用熔融晶体，例如熔融盐晶体来形成骨架。 与现有技术方法相比，本发明的支架的制造方法提高了孔隙率，互连性和制造容易度。
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公开(公告)号：US07338798B2
公开(公告)日：2008-03-04
申请号：US10663577
申请日：2003-09-16
申请人： Robert G. Dennis , Ravi K. Birla , Marvin O. Boluyl , Ellen M. Arruda , Keith R. Baar , Gregory H. Borschel
发明人： Robert G. Dennis , Ravi K. Birla , Marvin O. Boluyl , Ellen M. Arruda , Keith R. Baar , Gregory H. Borschel
IPC分类号： C12N5/00
CPC分类号： C12N5/0658 , A61K35/12 , C12N2533/52 , C12N2533/92
摘要： A system and method for forming a cardiac muscle construct are provided, the system including a substrate and cardiac cells provided on the substrate in the absence of a scaffold. The cardiac cells are cultured in vitro under conditions to allow the cells to become confluent and detach from the substrate to form a three-dimensional cardiac muscle construct.
摘要翻译： 提供了一种用于形成心肌构建体的系统和方法，所述系统包括在没有支架的情况下在基底上提供的底物和心脏细胞。 心肌细胞在体外培养条件下使细胞变得融合并与底物分离以形成三维心肌构建体。
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公开(公告)号：US06777234B1
公开(公告)日：2004-08-17
申请号：US09709890
申请日：2000-11-09
IPC分类号： C12N500
CPC分类号： C12N5/0658 , A61K35/12 , C12N2533/52
摘要： A mammalian muscle construct and a method for producing the construct are provided. The mammalian muscle construct includes a substrate and a plurality of separate anchors secured to the substrate. Myogenic precursor cells are provided on the substrate with at least some of the cells in contact with the anchors. The myogenic precursor cells are cultured in vitro under conditions to allow the cells to become confluent between the anchors. The anchors are receptive to the cells and allow the cells to attach thereto, such that placement of the anchors controls the size and shape of the muscle construct formed. Specifically, the anchors include separate fragments of biocompatible material secured to the substrate, wherein cell adhesion molecules are associated with each fragment to facilitate attachment of the precursor cells to the fragment.
摘要翻译： 提供哺乳动物肌肉构造物和制备该构建体的方法。 哺乳动物肌肉构造物包括基底和固定到基底的多个单独的锚。 在底物上提供生肌前体细胞，其中至少一些细胞与锚固体接触。 在体外培养肌原性前体细胞的条件下使细胞在锚定体之间变得融合。 锚固体接受细胞并允许细胞附着于其上，使得锚的放置控制形成的肌肉构造的大小和形状。 具体地，锚固体包括固定到底物的生物相容性材料的分开的片段，其中细胞粘附分子与每个片段相关联，以促进前体细胞与片段的连接。
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5.发明申请公开(公告)号：US20090082610A1
公开(公告)日：2009-03-26
申请号：US11859939
申请日：2007-09-24
申请人： David A. WOLF , Robert G. DENNIS , Donnie RUDD
发明人： David A. WOLF , Robert G. DENNIS , Donnie RUDD
IPC分类号： A61N2/00
摘要： An osteo or tissue healing device, kit and method of using the device to promote healing of compromised bone or tissue in a living mammal is disclosed. The osteo or tissue healing device includes a magnetic field emitter and a controlling circuit. The magnetic field emitter of the device is electrically coupled to a controlling circuit in which the magnetic field emitter is configured to provide a time variant magnetic field when driven by an electric pulse train from the controlling circuit in such a manner that the time variant magnetic field results in a magnetic (B) field exhibiting a magnetic slew rate of at least about 10 kiloGauss/sec. The controlling circuit of the device is electrically coupled to the magnetic field emitter and is configured to be powered by a power source, in which the controlling circuit is configured to output the electric pulse train driving the magnetic field emitter. It is thought that the steep magnetic field pulses and the relatively long inactive periods play important roles in promoting bone or tissue healing processes. The kit includes the unassembled components of the device. The method of using the osteo or tissue healing device includes the step of applying a time variant magnetic field to bone or tissue to promote healing of the bone or tissue.
摘要翻译： 公开了一种使用该装置促进活的哺乳动物中受损的骨骼或组织的愈合的骨或组织愈合装置，试剂盒和方法。 骨或组织愈合装置包括磁场发射器和控制电路。 器件的磁场发射极电耦合到控制电路，其中磁场发射器被配置为当由来自控制电路的电脉冲串驱动时提供时变磁场，使得时变磁场 导致表现出至少约10千高斯/秒的磁压摆率的磁（B）场。 该装置的控制电路电耦合到磁场发射器，并且被配置为由电源供电，其中控制电路被配置为输出驱动磁场发射器的电脉冲串。 据认为，陡峭的磁场脉冲和较长的非活动期在促进骨骼或组织愈合过程中起重要作用。 该套件包括设备的未组装组件。 使用骨或组织愈合装置的方法包括将时变磁场施加到骨骼或组织以促进骨骼或组织愈合的步骤。
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6.发明申请COMPOSITION AND METHOD FOR PRODUCING COMPOSITION FOR CONSTRUCTING TISSUE, AND TISSUE CONSTRUCT 审中-公开用于构造组织的组合物的组合物和方法，以及组织结构公开(公告)号：US20080138415A1
公开(公告)日：2008-06-12
申请号：US11859952
申请日：2007-09-24
申请人： Mehboob HUSSAIN , Robert G. DENNIS , David A. WOLF
发明人： Mehboob HUSSAIN , Robert G. DENNIS , David A. WOLF
CPC分类号： A61K35/28 , A61K31/573 , A61K35/12 , A61K38/1841 , A61K38/185 , A61K38/1866 , A61K38/193 , C12N5/00 , C12N5/0663 , C12N13/00 , C12N2525/00 , A61K2300/00
摘要： A cellular composition comprising a mesenchymal stem cell product and a biogel with methods for preparing and for using the composition. Tissue constructs made with the composition are also disclosed.
摘要翻译： 一种细胞组合物，其包含间充质干细胞产物和具有制备和使用该组合物的方法的生物凝胶。 还公开了用该组合物制备的组织结构。
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公开(公告)号：US20090082613A1
公开(公告)日：2009-03-26
申请号：US11859991
申请日：2007-09-24
申请人： Robert G. DENNIS , David A. WOLF , Donnie RUDD
发明人： Robert G. DENNIS , David A. WOLF , Donnie RUDD
CPC分类号： A61N2/02
摘要： An osteo or tissue healing kit and method of promoting the healing of compromised bone or tissue in a living mammal. The osteo or tissue healing device of the kit includes a magnetic field emitter and a controlling circuit, and at least one stem cell. The method of using the osteo or tissue healing kit includes placing at least one stem cell within the compromised bone or tissue and applying a time variant magnetic field.
摘要翻译： 一种骨骼或组织愈合试剂盒和促进活的哺乳动物中受损的骨骼或组织的愈合的方法。 该套件的骨组织愈合装置包括磁场发射器和控制电路以及至少一个干细胞。 使用骨或组织愈合试剂盒的方法包括将至少一个干细胞置于受损骨或组织内并应用时变磁场。
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公开(公告)号：US07422900B1
公开(公告)日：2008-09-09
申请号：US10602789
申请日：2003-06-24
IPC分类号： C12N5/00
CPC分类号： A61L27/3604 , A61K35/12 , A61L27/3804 , A61L27/386 , A61L27/3895 , C12N5/066 , C12N2500/38 , C12N2533/18 , C12N2533/52 , C12N2533/56
摘要： A system and method are provided for forming a connective tissue construct, such as a tendon construct, in vitro. A substrate is provided with at least two anchors secured thereto in spaced relationship. Fibroblast cells are provided on the substrate in the absence of a synthetic matrix, where at least some of the cells are in contact with the anchors. The cells are cultured in vitro under conditions to allow the cells to self-organize and become confluent between the anchors, where the anchors are receptive to the cells and allow the cells to attach thereto while permitting the cells to detach from the substrate to form a three-dimensional connective tissue construct.
摘要翻译： 提供了用于在体外形成结缔组织构建体（例如腱结构）的系统和方法。 基板具有至少两个以其间隔的关系固定到其上的锚固件。 在不存在合成基质的情况下，在基质上提供成纤维细胞，其中至少一些细胞与锚接触。 在体外培养细胞以允许细胞自我组织并在锚定体之间融合，其中锚固体接受细胞并允许细胞附着于细胞，同时允许细胞从基底分离以形成 三维结缔组织结构。
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9.发明授权公开(公告)号：US06303286B1
公开(公告)日：2001-10-16
申请号：US09611241
申请日：2000-07-06
申请人： Robert G. Dennis , Paul Kosnik
发明人： Robert G. Dennis , Paul Kosnik
IPC分类号： C12Q300
摘要： A system and method are provided for adaptively controlling a muscle tissue specimen in order to emulate its in vivo environment. The system includes at least one stimulator for stimulating the muscle tissue specimen based on an initial control signal, wherein the stimulation preferably includes electrical and/or mechanical stimulation. A response signal is generated based on a response of the muscle tissue specimen to the step of stimulating. The response signal preferably represents force production of the tissue specimen. A controller is provided for modifying the initial control signal based on the response signal to obtain a final control signal, wherein the final control signal is used to elicit a desired response from the muscle tissue specimen. Advantageously, the system and method of the present invention can be used to adaptively control the stimulation of a muscle tissue specimen in a tissue culture environment.
摘要翻译： 提供了一种用于自适应地控制肌肉组织样本以便模拟其体内环境的系统和方法。 该系统包括至少一个用于基于初始控制信号刺激肌肉组织样本的刺激器，其中所述刺激优选地包括电和/或机械刺激。 基于肌肉组织样本对刺激步骤的响应产生响应信号。 响应信号优选地表示组织标本的力产生。 提供控制器，用于基于响应信号修改初始控制信号以获得最终控制信号，其中最终控制信号用于从肌肉组织标本引出期望的响应。 有利地，本发明的系统和方法可用于适应性地控制组织培养环境中肌肉组织标本的刺激。
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公开(公告)号：US20090081752A1
公开(公告)日：2009-03-26
申请号：US11859976
申请日：2007-09-24
申请人： Robert G. DENNIS , David A. WOLF , Donnie RUDD
发明人： Robert G. DENNIS , David A. WOLF , Donnie RUDD
CPC分类号： C12N5/0634 , A61K2035/124 , C12N2525/00
摘要： A bioreactor, kit, a method of using the device to promoting growing and/or culture of a cell, and a method for regenerating and/or improving the function of mammalian cells. The bioreactor includes a controlling circuit coupled to magnetic field emitter that emits relatively steep and short-lived magnetic field pulses during these an active ephemeral period. The bioreactor also provides a relatively long-term inactive phase in which no magnetic field pulses are imposed. The kit includes the unassembled components of the bioreactor. The method of using the bioreactor and of regenerating and/or improving the function of mammalian cells includes the step of applying a time variant magnetic field through the cell to promote growing and/or culturing, and then introducing the cells to a mammal to regenerate cells.
摘要翻译： 生物反应器，试剂盒，使用该装置促进细胞生长和/或培养的方法，以及用于再生和/或改善哺乳动物细胞功能的方法。 生物反应器包括耦合到磁场发射器的控制电路，其在这些活跃的短暂时段期间发射相对陡峭和短寿命的磁场脉冲。 生物反应器还提供了不施加磁场脉冲的相对长期的非活性阶段。 试剂盒包括生物反应器的未组装的组分。 使用生物反应器和再生和/或改善哺乳动物细胞功能的方法包括将时变磁场通过细胞以促进生长和/或培养，然后将细胞引入哺乳动物以再生细胞的步骤 。
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