公开(公告)号：US4787963A

公开(公告)日：1988-11-29

申请号：US46652

申请日：1987-05-04

申请人： William P. MacConnell

发明人： William P. MacConnell

IPC分类号： C12Q1/68 ,  C12N15/00

CPC分类号： C12Q1/6813 ,  C12Q1/68

摘要： Method and means for accelerating the rate of hybridization of nucleic acid probes with complemental target sequences in probe assays of the filter binding or sandwich filter binding formats. The nucleic acid probes are electrophoretically concentrated at membrane means including at least a dialysis type membrane and to which the target sequences are bound. The rate of hybridization can be further enhanced by means for moving concentrated unannealed probe molecules successively in various directions along the surface of the membrane means to which the target sequences are bound. The invention further includes method and means for electrophoretically removing from the membrane means unannealed probe molecules which became adsorbed to the membrane means during hybridization. In one embodiment of the invention the membrane means comprises a cellulose dialysis membrane in laminate relation with a nitrocellulose or nylon filter having target sequence molecules bound to the surface thereof facing the dialysis membrane. In another embodiment of the invention the membrane means comprises a dialysis membrane to one side of which the target sequence molecules are bound.

摘要翻译： 用于在过滤器结合或夹心过滤器结合形式的探针测定中加速核酸探针与补体靶序列的杂交速率的方法和装置。 核酸探针在包括至少透析型膜的膜装置处电泳浓缩，并且靶序列与之结合。 通过使浓缩的未退火的探针分子在沿着靶序列所结合的膜装置的表面的各个方向依次移动，可以进一步提高杂交速率。 本发明还包括用于从膜电泳去除的方法和手段，意味着在杂交期间被吸附到膜上的未退火的探针分子。 在本发明的一个实施方案中，膜装置包括与硝化纤维素或尼龙过滤器层压关系的纤维素透析膜，其具有与其面向透析膜的表面结合的靶序列分子。 在本发明的另一个实施方案中，膜装置包括透析膜，其一侧的靶序列分子被结合。