公开(公告)号：US20220404245A1

公开(公告)日：2022-12-22

申请号：US17889025

申请日：2022-08-16

Applicant: 10x Genomics, Inc.

Inventor： James Michael Chell ,  Joshua Delaney ,  Rapolas Spalinskas ,  Aleksandra Jurek ,  Meiliana Tjandra

IPC: G01N1/30

Abstract: Provided herein are methods for de-crosslinking fixed biological samples (e.g., fixed biological samples including aminal crosslinks). The compositions and methods disclosed can de-crosslink oligonucleotides (e.g., DNA or RNA) or proteins from fixed biological samples (e.g., fixed biological samples with aminal crosslinks), wherein the de-crosslinked biological sample is compatible with and can be used in spatial gene expression analysis.

公开(公告)号：US20220334031A1

公开(公告)日：2022-10-20

申请号：US17845331

申请日：2022-06-21

Applicant: 10x Genomics, Inc.

Inventor： Joshua Delaney ,  Shalini Gohil ,  Adam Lowe ,  Yi Luo ,  Dagmar Walter

IPC: G01N1/30 ,  C07D403/12

Abstract: The present disclosure relates to compositions and methods for reversible fixation of biological samples using fixation reagents that form bis-carbamate crosslinks between amine-bearing moieties in biomolecules.

公开(公告)号：US12241890B2

公开(公告)日：2025-03-04

申请号：US17131174

申请日：2020-12-22

Applicant: 10X Genomics, Inc.

Inventor： Joshua Delaney ,  Shalini Gohil ,  Jill Herschleb ,  Adam Lowe ,  Albert Kim ,  Meiliana Tjandra

IPC: C12N15/11 ,  C07D213/04 ,  C07D239/24 ,  C12N9/48 ,  G01N33/50 ,  G01N33/53 ,  G01N1/30

Abstract: The present disclosure provides compositions and methods for using fixed biological samples in partition-based assays. In at least one embodiment, the disclosure provides a composition comprising a fixed biological sample and an un-fixing agent contained in a partition, such as a discrete droplet. In some embodiments, the disclosure provides un-fixing agent compounds capable of catalytically cleaving crosslinks in fixed biological samples, particularly crosslinked nucleic acids, such as RNA.

公开(公告)号：US20250003956A1

公开(公告)日：2025-01-02

申请号：US18828673

申请日：2024-09-09

Applicant: 10x Genomics, Inc.

Inventor： Joshua Delaney ,  Shalini Gohil ,  Jill Herschleb ,  Adam Lowe ,  Albert Kim ,  Meiliana Tjandra

IPC: G01N33/53 ,  C07D213/04 ,  C07D239/24 ,  C12N9/48 ,  G01N1/30 ,  G01N33/50

Abstract: The present disclosure provides compositions and methods for using fixed biological samples in partition-based assays. In at least one embodiment, the disclosure provides a composition comprising a fixed biological sample and an un-fixing agent contained in a partition, such as a discrete droplet. In some embodiments, the disclosure provides un-fixing agent compounds capable of catalytically cleaving crosslinks in fixed biological samples, particularly crosslinked nucleic acids, such as RNA.

公开(公告)号：US20240125772A1

公开(公告)日：2024-04-18

申请号：US18503564

申请日：2023-11-07

Applicant: 10x Genomics, Inc.

Inventor： Joshua Delaney ,  Shalini Gohil ,  Jill Herschleb ,  Adam Lowe ,  Albert Kim ,  Meiliana Tjandra

IPC: G01N33/53 ,  C07D213/04 ,  C07D239/24 ,  C12N9/48 ,  G01N33/50

CPC classification number: G01N33/5308 ,  C07D213/04 ,  C07D239/24 ,  C12N9/48 ,  G01N33/5047 ,  G01N2001/307 ,  G01N2333/948

Abstract: The present disclosure provides compositions and methods for using fixed biological samples in partition-based assays. In at least one embodiment, the disclosure provides a composition comprising a fixed biological sample and an un-fixing agent contained in a partition, such as a discrete droplet. In some embodiments, the disclosure provides un-fixing agent compounds capable of catalytically cleaving crosslinks in fixed biological samples, particularly crosslinked nucleic acids, such as RNA.

公开(公告)号：US11441172B2

公开(公告)日：2022-09-13

申请号：US17009352

申请日：2020-09-01

Applicant: 10X Genomics, Inc.

Inventor： Joshua Delaney ,  Shalini Gohil ,  Christopher Hindson ,  Adam Lowe ,  Andrew D. Price ,  Joseph Francis Shuga

IPC: C12Q1/6806 ,  B01J13/18 ,  B01J13/00 ,  C08J3/075 ,  C12Q1/6869

Abstract: The present disclosure provides systems and methods for making a hydrogel comprising a cell, cell nucleus, or one or more components derived from a cell or cell nucleus. A method for making a hydrogel may comprise providing a cell or cell nucleus, a first polymer, wherein the first polymer comprises a plurality of first crosslink precursors, each of the plurality of first crosslink precursors comprising an azide group; providing a second polymer, wherein the second polymer comprises a plurality of second crosslink precursors, each of the plurality of second crosslink precursors comprising an alkyne group; and crosslinking the first polymer and the second polymer via a reaction between a first section of the first crosslink precursors and a second section of the second crosslink precursors, thereby providing the hydrogel comprising the cell or cell nucleus.

公开(公告)号：US10774369B2

公开(公告)日：2020-09-15

申请号：US16374112

申请日：2019-04-03

Applicant: 10X Genomics, Inc.

Inventor： Joshua Delaney ,  Shalini Gohil ,  Christopher Hindson ,  Adam Lowe ,  Andrew D. Price ,  Joseph Francis Shuga

IPC: C08J3/075 ,  C12Q1/6806 ,  B01J13/18 ,  B01J13/00 ,  C12Q1/6869

Abstract: The present disclosure provides systems and methods for making a hydrogel comprising a cell, cell nucleus, or one or more components derived from a cell or cell nucleus. A method for making a hydrogel may comprise providing a cell or cell nucleus, a first polymer, wherein the first polymer comprises a plurality of first crosslink precursors, each of the plurality of first crosslink precursors comprising an azide group; providing a second polymer, wherein the second polymer comprises a plurality of second crosslink precursors, each of the plurality of second crosslink precursors comprising an alkyne group; and crosslinking the first polymer and the second polymer via a reaction between a first section of the first crosslink precursors and a second section of the second crosslink precursors, thereby providing the hydrogel comprising the cell or cell nucleus.