公开(公告)号：US20220033888A1

公开(公告)日：2022-02-03

申请号：US17312247

申请日：2019-12-06

Applicant: 10x Genomics, Inc.

Inventor： Michael Schnall-Levin ,  Eswar Prasad Ramachandran Iyer ,  Yifeng Yin ,  Tarjei Sigurd Mikkelsen ,  Preyas Shah ,  Geoffrey McDermott ,  Adam Lowe ,  Joshua Delaney ,  Augusto Manuel Tentori ,  Rajiv Bharadwaj ,  Stefania Giacomello ,  Alexander Gagnon ,  Meghan L. F. Frey ,  Zachary Bent ,  Erik Leonard Henrik Borgstrom

IPC: C12Q1/6837 ,  C12Q1/6881

Abstract: The present disclosure relates to compositions and methods for generating capture probes on a substrate for identifying the location of analytes in a biological sample.

公开(公告)号：US10590244B2

公开(公告)日：2020-03-17

申请号：US16178430

申请日：2018-11-01

Applicant: 10X Genomics, Inc.

Inventor： Joshua Delaney ,  Shalini Gohil ,  Christopher Hindson ,  Adam Lowe ,  Andrew D. Price

IPC: C08J3/075 ,  C08J3/24 ,  B01L3/00

Abstract: The present disclosure provides systems and methods for making a hydrogel comprising a cell, cell nucleus, or one or more components derived from a cell or cell nucleus. A method for making a hydrogel may comprise providing a cell or cell nucleus, a first polymer, wherein the first polymer comprises a plurality of first crosslink precursors, each of the plurality of first crosslink precursors comprising an azide group; providing a second polymer, wherein the second polymer comprises a plurality of second crosslink precursors, each of the plurality of second crosslink precursors comprising an alkyne group; and crosslinking the first polymer and the second polymer via a reaction between a first section of the first crosslink precursors and a second section of the second crosslink precursors, thereby providing the hydrogel comprising the cell or cell nucleus.

公开(公告)号：US12117439B2

公开(公告)日：2024-10-15

申请号：US18503564

申请日：2023-11-07

Applicant: 10x Genomics, Inc.

Inventor： Joshua Delaney ,  Shalini Gohil ,  Jill Herschleb ,  Adam Lowe ,  Albert Kim ,  Meiliana Tjandra

IPC: G01N33/53 ,  C07D213/04 ,  C07D239/24 ,  C12N9/48 ,  G01N33/50 ,  G01N1/30

CPC classification number: G01N33/5308 ,  C07D213/04 ,  C07D239/24 ,  C12N9/48 ,  G01N33/5047 ,  G01N2001/307 ,  G01N2333/948

Abstract: The present disclosure provides compositions and methods for using fixed biological samples in partition-based assays. In at least one embodiment, the disclosure provides a composition comprising a fixed biological sample and an un-fixing agent contained in a partition, such as a discrete droplet. In some embodiments, the disclosure provides un-fixing agent compounds capable of catalytically cleaving crosslinks in fixed biological samples, particularly crosslinked nucleic acids, such as RNA.

公开(公告)号：US20240043914A1

公开(公告)日：2024-02-08

申请号：US18366621

申请日：2023-08-07

Applicant: 10X Genomics, Inc.

Inventor： Hong CHEN ,  Joshua Delaney ,  Yi Luo ,  Meiliana Tjandra

IPC: C12Q1/6841 ,  C12Q1/6876 ,  G01N21/64 ,  G01N1/30 ,  C12Q1/6806

CPC classification number: C12Q1/6841 ,  C12Q1/6876 ,  G01N21/64 ,  G01N1/30 ,  C12Q1/6806 ,  G01N2021/6432 ,  G01N2021/6439

Abstract: The present disclosure generally relates to methods and compositions for in situ analysis or detection of analytes in a biological sample. More specifically, the present disclosure relates to methods for reducing autofluorescence in tissue samples, methods for analyzing tissue samples, and compounds for use in the same. The methods and compounds of the present disclosure may be especially suitable for analytical methods employing fluorescence in situ hybridization techniques over multiple cycles of imaging.

公开(公告)号：US11884964B2

公开(公告)日：2024-01-30

申请号：US17861503

申请日：2022-07-11

Applicant: 10x Genomics, Inc.

Inventor： Joshua Delaney ,  Shalini Gohil ,  Christopher Hindson ,  Adam Lowe ,  Andrew D. Price ,  Joseph Francis Shuga

IPC: C12Q1/6806 ,  B01J13/18 ,  B01J13/00 ,  C08J3/075 ,  C12Q1/6869

CPC classification number: C12Q1/6806 ,  B01J13/0065 ,  B01J13/18 ,  C08J3/075 ,  C08J2333/00 ,  C12Q1/6869 ,  C12Q1/6869 ,  C12Q2563/159 ,  C12Q2565/629

Abstract: The present disclosure provides systems and methods for making a hydrogel comprising a cell, cell nucleus, or one or more components derived from a cell or cell nucleus. A method for making a hydrogel may comprise providing a cell or cell nucleus, a first polymer, wherein the first polymer comprises a plurality of first crosslink precursors, each of the plurality of first crosslink precursors comprising an azide group; providing a second polymer, wherein the second polymer comprises a plurality of second crosslink precursors, each of the plurality of second crosslink precursors comprising an alkyne group; and crosslinking the first polymer and the second polymer via a reaction between a first section of the first crosslink precursors and a second section of the second crosslink precursors, thereby providing the hydrogel comprising the cell or cell nucleus.

公开(公告)号：US12234507B2

公开(公告)日：2025-02-25

申请号：US18366621

申请日：2023-08-07

Applicant: 10x Genomics, Inc.

Inventor： Hong Chen ,  Joshua Delaney ,  Yi Luo ,  Meiliana Tjandra

IPC: C12Q1/6841 ,  C12Q1/6806 ,  C12Q1/6876 ,  G01N1/30 ,  G01N21/64

Abstract: The present disclosure generally relates to methods and compositions for in situ analysis or detection of analytes in a biological sample. More specifically, the present disclosure relates to methods for reducing autofluorescence in tissue samples, methods for analyzing tissue samples, and compounds for use in the same. The methods and compounds of the present disclosure may be especially suitable for analytical methods employing fluorescence in situ hybridization techniques over multiple cycles of imaging.

公开(公告)号：US20230287475A1

公开(公告)日：2023-09-14

申请号：US18018101

申请日：2020-12-22

Applicant: 10x Genomics, Inc.

Inventor： James Michael Chell ,  Joshua Delaney ,  Rapolas Spalinskas ,  Aleksandra Jurek ,  Meiliana Tjandra

IPC: C12Q1/6806

CPC classification number: C12Q1/6806

Abstract: Provided herein are methods for de-crosslinking fixed biological samples (e.g., fixed biological samples including aminal crosslinks). The compositions and methods disclosed can de-crosslink oligonucleotides (e.g., DNA or RNA) or proteins from fixed biological samples (e.g., fixed biological samples with aminal crosslinks), wherein the de-crosslinked biological sample is compatible with and can be used in spatial gene expression analysis.

公开(公告)号：US20190100632A1

公开(公告)日：2019-04-04

申请号：US16178430

申请日：2018-11-01

Applicant: 10X Genomics, Inc.

Inventor： Joshua Delaney ,  Shalini Gohil ,  Christopher Hindson ,  Adam Lowe ,  Andrew D. Price

IPC: C08J3/075 ,  B01L3/00

Abstract: The present disclosure provides systems and methods for making a hydrogel comprising a cell, cell nucleus, or one or more components derived from a cell or cell nucleus. A method for making a hydrogel may comprise providing a cell or cell nucleus, a first polymer, wherein the first polymer comprises a plurality of first crosslink precursors, each of the plurality of first crosslink precursors comprising an azide group; providing a second polymer, wherein the second polymer comprises a plurality of second crosslink precursors, each of the plurality of second crosslink precursors comprising an alkyne group; and crosslinking the first polymer and the second polymer via a reaction between a first section of the first crosslink precursors and a second section of the second crosslink precursors, thereby providing the hydrogel comprising the cell or cell nucleus.

公开(公告)号：US12258624B2

公开(公告)日：2025-03-25

申请号：US18499129

申请日：2023-10-31

Applicant: 10x Genomics, Inc.

Inventor： Joshua Delaney ,  Shalini Gohil ,  Veronica Emelina Gonzalez Muñoz ,  Joshua Gu ,  Albert Dale Kim ,  Yi Luo ,  Tathagata Mukherjee ,  Monica Nagendran ,  James Francis Perna, III ,  Kristen Nguyen Pham ,  Meiliana Tjandra

IPC: C12Q1/68 ,  C12Q1/6806 ,  C12Q1/6816 ,  C12Q1/6823 ,  C12Q1/6841 ,  C12Q1/6876

Abstract: The present disclosure relates in some aspects to methods and compositions for in situ analysis involving catalytic de-crosslinking of biological samples.

公开(公告)号：US20230279481A1

公开(公告)日：2023-09-07

申请号：US18196835

申请日：2023-05-12

Applicant: 10x Genomics, Inc.

Inventor： Sean Marrache ,  Shalini Gohil ,  Joshua Delaney ,  Paul Eugene Lund

IPC: C12Q1/6855

CPC classification number: C12Q1/6855

Abstract: The present disclosure provides compositions, methods, and kits comprising a nano-partition contained within a partition that allows for the separation and use of at least two enzymes in a partition-based assay of a biological sample, such as a single cell in a droplet. The compositions and methods are useful for carrying out partition-based assays of fixed biological samples.