公开(公告)号：US20220404245A1

公开(公告)日：2022-12-22

申请号：US17889025

申请日：2022-08-16

Applicant: 10x Genomics, Inc.

Inventor： James Michael Chell ,  Joshua Delaney ,  Rapolas Spalinskas ,  Aleksandra Jurek ,  Meiliana Tjandra

IPC: G01N1/30

Abstract: Provided herein are methods for de-crosslinking fixed biological samples (e.g., fixed biological samples including aminal crosslinks). The compositions and methods disclosed can de-crosslink oligonucleotides (e.g., DNA or RNA) or proteins from fixed biological samples (e.g., fixed biological samples with aminal crosslinks), wherein the de-crosslinked biological sample is compatible with and can be used in spatial gene expression analysis.

公开(公告)号：US11332790B2

公开(公告)日：2022-05-17

申请号：US17220529

申请日：2021-04-01

Applicant: 10x Genomics, Inc.

Inventor： James Michael Chell ,  Marlon Stoeckius ,  Jonathan Alles ,  Caroline Julie Gallant ,  Christina Galonska ,  Felice Alessio Bava ,  Layla Katiraee

IPC: C12Q1/6876 ,  C12Q1/6811 ,  C12Q1/25 ,  C12Q1/6816 ,  C12Q1/6841 ,  C12Q1/6851

Abstract: Provided herein are methods of detecting an analyte of interest to interrogate spatial gene expression in a sample using RNA-templated ligation.

公开(公告)号：US20230287475A1

公开(公告)日：2023-09-14

申请号：US18018101

申请日：2020-12-22

Applicant: 10x Genomics, Inc.

Inventor： James Michael Chell ,  Joshua Delaney ,  Rapolas Spalinskas ,  Aleksandra Jurek ,  Meiliana Tjandra

IPC: C12Q1/6806

CPC classification number: C12Q1/6806

Abstract: Provided herein are methods for de-crosslinking fixed biological samples (e.g., fixed biological samples including aminal crosslinks). The compositions and methods disclosed can de-crosslink oligonucleotides (e.g., DNA or RNA) or proteins from fixed biological samples (e.g., fixed biological samples with aminal crosslinks), wherein the de-crosslinked biological sample is compatible with and can be used in spatial gene expression analysis.

公开(公告)号：US20230064372A1

公开(公告)日：2023-03-02

申请号：US17896520

申请日：2022-08-26

Applicant: 10x Genomics, Inc.

Inventor： James Michael Chell ,  Marlon Stoeckius ,  Jonathan Alles ,  Caroline Julie Gallant ,  Christina Galonska ,  Layla Katiraee

IPC: C12Q1/6876 ,  C12Q1/6811 ,  C12Q1/25 ,  C12Q1/6816 ,  C12Q1/6841 ,  C12Q1/6851

Abstract: Provided herein are methods of detecting an analyte of interest to interrogate spatial gene expression in a sample using RNA-templated ligation.

公开(公告)号：US20220326251A1

公开(公告)日：2022-10-13

申请号：US17738808

申请日：2022-05-06

Applicant: 10x Genomics, Inc.

Inventor： Cedric Uytingco ,  James Michael Chell ,  Marlon Stoeckius ,  Albert Kim ,  Dulce Ovando Morales ,  Andrej Hartnett ,  Yifeng Yin ,  Jennifer Chew

IPC: G01N33/68 ,  C12N15/10 ,  C12Q1/6804 ,  G01N33/58 ,  C12Q1/6874 ,  G01N1/30 ,  G01N21/64

Abstract: Provided herein are methods for preparing biological samples for spatial proteomic analysis, methods of determining a location of a protein analyte in a biological sample, and methods of determining a location of a protein analyte and a nucleic acid analyte in a biological sample.

公开(公告)号：US20220010367A1

公开(公告)日：2022-01-13

申请号：US17459931

申请日：2021-08-27

Applicant: 10x Genomics, Inc.

Inventor： Eswar Prasad Ramachandran Iyer ,  Tarjei Sigurd Mikkelsen ,  Augusto Manuel Tentori ,  Rajiv Bharadwaj ,  Marlon Stoeckius ,  James Michael Chell ,  Cedric Uytingco

IPC: C12Q1/6841 ,  C12N15/10 ,  G01N33/53 ,  C12Q1/6804

Abstract: This disclosure relates to methods for spatial profiling of analytes present in a biological sample. Also provided are methods for using spatially barcoded substrates to detect a biological analyte in a cell culture, an organism, and organoid. Also provided are methods for using spatially barcoded substrates to detect the temporal profile of a biological analyte.

公开(公告)号：US20250154571A1

公开(公告)日：2025-05-15

申请号：US19021909

申请日：2025-01-15

Applicant: 10x Genomics, Inc.

Inventor： Eswar Prasad Ramachandran Iyer ,  Tarjei Sigurd Mikkelsen ,  Augusto Manuel Tentori ,  Rajiv Bharadwaj ,  Marlon Stoeckius ,  James Michael Chell ,  Cedric Uytingco

IPC: C12Q1/6841 ,  C12Q1/6874 ,  C12Q1/6886 ,  G16B30/10

Abstract: This disclosure relates to methods for spatial profiling of analytes present in a biological sample. Also provided are methods for using spatially barcoded substrates to detect a biological analyte in a cell culture, an organism, and organoid. Also provided are methods for using spatially barcoded substrates to detect the temporal profile of a biological analyte.

公开(公告)号：US11808769B2

公开(公告)日：2023-11-07

申请号：US18160084

申请日：2023-01-26

Applicant: 10x Genomics, Inc.

Inventor： Cedric Uytingco ,  James Michael Chell ,  Marlon Stoeckius ,  Albert Dale Kim ,  Dulce Ovando Morales ,  Andrej Hartnett ,  Yifeng Yin ,  Jennifer Chew

IPC: G01N33/68 ,  C12N15/10 ,  C12Q1/6804 ,  C12Q1/6874 ,  G01N1/30 ,  G01N21/64 ,  G01N33/58

CPC classification number: G01N33/6803 ,  C12N15/1065 ,  C12Q1/6804 ,  C12Q1/6874 ,  G01N1/30 ,  G01N21/6428 ,  G01N21/6456 ,  G01N33/582 ,  G01N2021/6439

Abstract: Provided herein are methods for preparing biological samples for spatial proteomic analysis, methods of determining a location of a protein analyte in a biological sample, and methods of determining a location of a protein analyte and a nucleic acid analyte in a biological sample.

公开(公告)号：US11795507B2

公开(公告)日：2023-10-24

申请号：US17896520

申请日：2022-08-26

Applicant: 10x Genomics, Inc.

Inventor： James Michael Chell ,  Marlon Stoeckius ,  Jonathan Alles ,  Caroline Julie Gallant ,  Christina Galonska ,  Layla Katiraee

IPC: C12Q1/68 ,  C12Q1/6876 ,  C12Q1/6811 ,  C12Q1/25 ,  C12Q1/6816 ,  C12Q1/6841 ,  C12Q1/6851

CPC classification number: C12Q1/6876 ,  C12Q1/25 ,  C12Q1/6811 ,  C12Q1/6816 ,  C12Q1/6841 ,  C12Q1/6851

Abstract: Provided herein are methods of detecting an analyte of interest to interrogate spatial gene expression in a sample using RNA-templated ligation.

公开(公告)号：US11592447B2

公开(公告)日：2023-02-28

申请号：US17738808

申请日：2022-05-06

Applicant: 10x Genomics, Inc.

Inventor： Cedric Uytingco ,  James Michael Chell ,  Marlon Stoeckius ,  Albert Kim ,  Dulce Ovando Morales ,  Andrej Hartnett ,  Yifeng Yin ,  Jennifer Chew

IPC: G01N33/68 ,  C12N15/10 ,  C12Q1/6804 ,  C12Q1/6874 ,  G01N1/30 ,  G01N21/64 ,  G01N33/58

Abstract: Provided herein are methods for preparing biological samples for spatial proteomic analysis, methods of determining a location of a protein analyte in a biological sample, and methods of determining a location of a protein analyte and a nucleic acid analyte in a biological sample.