公开(公告)号：US20220033888A1

公开(公告)日：2022-02-03

申请号：US17312247

申请日：2019-12-06

Applicant: 10x Genomics, Inc.

Inventor： Michael Schnall-Levin ,  Eswar Prasad Ramachandran Iyer ,  Yifeng Yin ,  Tarjei Sigurd Mikkelsen ,  Preyas Shah ,  Geoffrey McDermott ,  Adam Lowe ,  Joshua Delaney ,  Augusto Manuel Tentori ,  Rajiv Bharadwaj ,  Stefania Giacomello ,  Alexander Gagnon ,  Meghan L. F. Frey ,  Zachary Bent ,  Erik Leonard Henrik Borgstrom

IPC: C12Q1/6837 ,  C12Q1/6881

Abstract: The present disclosure relates to compositions and methods for generating capture probes on a substrate for identifying the location of analytes in a biological sample.

公开(公告)号：US20210040535A1

公开(公告)日：2021-02-11

申请号：US16915493

申请日：2020-06-29

Applicant: 10X GENOMICS, INC.

Inventor： Adam Lowe ,  Christopher Hindson ,  Andrew D. Price

IPC: C12Q1/6806 ,  C12N15/10 ,  B01J19/00 ,  B01J13/04

Abstract: The present disclosure provides particles (e.g., beads) and methods, kits, and systems involving the same for sample processing or analysis. Such particles may include one or more analytes, one or more reagents, and two or more gel components and/or walled components. The particles described herein may be formed, for example, by polymerization of a polymerizable material in proximity to a gel or walled component.

公开(公告)号：US10590244B2

公开(公告)日：2020-03-17

申请号：US16178430

申请日：2018-11-01

Applicant: 10X Genomics, Inc.

Inventor： Joshua Delaney ,  Shalini Gohil ,  Christopher Hindson ,  Adam Lowe ,  Andrew D. Price

IPC: C08J3/075 ,  C08J3/24 ,  B01L3/00

Abstract: The present disclosure provides systems and methods for making a hydrogel comprising a cell, cell nucleus, or one or more components derived from a cell or cell nucleus. A method for making a hydrogel may comprise providing a cell or cell nucleus, a first polymer, wherein the first polymer comprises a plurality of first crosslink precursors, each of the plurality of first crosslink precursors comprising an azide group; providing a second polymer, wherein the second polymer comprises a plurality of second crosslink precursors, each of the plurality of second crosslink precursors comprising an alkyne group; and crosslinking the first polymer and the second polymer via a reaction between a first section of the first crosslink precursors and a second section of the second crosslink precursors, thereby providing the hydrogel comprising the cell or cell nucleus.

公开(公告)号：US12241890B2

公开(公告)日：2025-03-04

申请号：US17131174

申请日：2020-12-22

Applicant: 10X Genomics, Inc.

Inventor： Joshua Delaney ,  Shalini Gohil ,  Jill Herschleb ,  Adam Lowe ,  Albert Kim ,  Meiliana Tjandra

IPC: C12N15/11 ,  C07D213/04 ,  C07D239/24 ,  C12N9/48 ,  G01N33/50 ,  G01N33/53 ,  G01N1/30

Abstract: The present disclosure provides compositions and methods for using fixed biological samples in partition-based assays. In at least one embodiment, the disclosure provides a composition comprising a fixed biological sample and an un-fixing agent contained in a partition, such as a discrete droplet. In some embodiments, the disclosure provides un-fixing agent compounds capable of catalytically cleaving crosslinks in fixed biological samples, particularly crosslinked nucleic acids, such as RNA.

公开(公告)号：US20250003956A1

公开(公告)日：2025-01-02

申请号：US18828673

申请日：2024-09-09

Applicant: 10x Genomics, Inc.

Inventor： Joshua Delaney ,  Shalini Gohil ,  Jill Herschleb ,  Adam Lowe ,  Albert Kim ,  Meiliana Tjandra

IPC: G01N33/53 ,  C07D213/04 ,  C07D239/24 ,  C12N9/48 ,  G01N1/30 ,  G01N33/50

Abstract: The present disclosure provides compositions and methods for using fixed biological samples in partition-based assays. In at least one embodiment, the disclosure provides a composition comprising a fixed biological sample and an un-fixing agent contained in a partition, such as a discrete droplet. In some embodiments, the disclosure provides un-fixing agent compounds capable of catalytically cleaving crosslinks in fixed biological samples, particularly crosslinked nucleic acids, such as RNA.

公开(公告)号：US20240125772A1

公开(公告)日：2024-04-18

申请号：US18503564

申请日：2023-11-07

Applicant: 10x Genomics, Inc.

Inventor： Joshua Delaney ,  Shalini Gohil ,  Jill Herschleb ,  Adam Lowe ,  Albert Kim ,  Meiliana Tjandra

IPC: G01N33/53 ,  C07D213/04 ,  C07D239/24 ,  C12N9/48 ,  G01N33/50

CPC classification number: G01N33/5308 ,  C07D213/04 ,  C07D239/24 ,  C12N9/48 ,  G01N33/5047 ,  G01N2001/307 ,  G01N2333/948

Abstract: The present disclosure provides compositions and methods for using fixed biological samples in partition-based assays. In at least one embodiment, the disclosure provides a composition comprising a fixed biological sample and an un-fixing agent contained in a partition, such as a discrete droplet. In some embodiments, the disclosure provides un-fixing agent compounds capable of catalytically cleaving crosslinks in fixed biological samples, particularly crosslinked nucleic acids, such as RNA.

公开(公告)号：US11441172B2

公开(公告)日：2022-09-13

申请号：US17009352

申请日：2020-09-01

Applicant: 10X Genomics, Inc.

Inventor： Joshua Delaney ,  Shalini Gohil ,  Christopher Hindson ,  Adam Lowe ,  Andrew D. Price ,  Joseph Francis Shuga

IPC: C12Q1/6806 ,  B01J13/18 ,  B01J13/00 ,  C08J3/075 ,  C12Q1/6869

Abstract: The present disclosure provides systems and methods for making a hydrogel comprising a cell, cell nucleus, or one or more components derived from a cell or cell nucleus. A method for making a hydrogel may comprise providing a cell or cell nucleus, a first polymer, wherein the first polymer comprises a plurality of first crosslink precursors, each of the plurality of first crosslink precursors comprising an azide group; providing a second polymer, wherein the second polymer comprises a plurality of second crosslink precursors, each of the plurality of second crosslink precursors comprising an alkyne group; and crosslinking the first polymer and the second polymer via a reaction between a first section of the first crosslink precursors and a second section of the second crosslink precursors, thereby providing the hydrogel comprising the cell or cell nucleus.

公开(公告)号：US10774369B2

公开(公告)日：2020-09-15

申请号：US16374112

申请日：2019-04-03

Applicant: 10X Genomics, Inc.

Inventor： Joshua Delaney ,  Shalini Gohil ,  Christopher Hindson ,  Adam Lowe ,  Andrew D. Price ,  Joseph Francis Shuga

IPC: C08J3/075 ,  C12Q1/6806 ,  B01J13/18 ,  B01J13/00 ,  C12Q1/6869

Abstract: The present disclosure provides systems and methods for making a hydrogel comprising a cell, cell nucleus, or one or more components derived from a cell or cell nucleus. A method for making a hydrogel may comprise providing a cell or cell nucleus, a first polymer, wherein the first polymer comprises a plurality of first crosslink precursors, each of the plurality of first crosslink precursors comprising an azide group; providing a second polymer, wherein the second polymer comprises a plurality of second crosslink precursors, each of the plurality of second crosslink precursors comprising an alkyne group; and crosslinking the first polymer and the second polymer via a reaction between a first section of the first crosslink precursors and a second section of the second crosslink precursors, thereby providing the hydrogel comprising the cell or cell nucleus.

公开(公告)号：US12117439B2

公开(公告)日：2024-10-15

申请号：US18503564

申请日：2023-11-07

Applicant: 10x Genomics, Inc.

Inventor： Joshua Delaney ,  Shalini Gohil ,  Jill Herschleb ,  Adam Lowe ,  Albert Kim ,  Meiliana Tjandra

IPC: G01N33/53 ,  C07D213/04 ,  C07D239/24 ,  C12N9/48 ,  G01N33/50 ,  G01N1/30

CPC classification number: G01N33/5308 ,  C07D213/04 ,  C07D239/24 ,  C12N9/48 ,  G01N33/5047 ,  G01N2001/307 ,  G01N2333/948

Abstract: The present disclosure provides compositions and methods for using fixed biological samples in partition-based assays. In at least one embodiment, the disclosure provides a composition comprising a fixed biological sample and an un-fixing agent contained in a partition, such as a discrete droplet. In some embodiments, the disclosure provides un-fixing agent compounds capable of catalytically cleaving crosslinks in fixed biological samples, particularly crosslinked nucleic acids, such as RNA.

公开(公告)号：US11884964B2

公开(公告)日：2024-01-30

申请号：US17861503

申请日：2022-07-11

Applicant: 10x Genomics, Inc.

Inventor： Joshua Delaney ,  Shalini Gohil ,  Christopher Hindson ,  Adam Lowe ,  Andrew D. Price ,  Joseph Francis Shuga

IPC: C12Q1/6806 ,  B01J13/18 ,  B01J13/00 ,  C08J3/075 ,  C12Q1/6869

CPC classification number: C12Q1/6806 ,  B01J13/0065 ,  B01J13/18 ,  C08J3/075 ,  C08J2333/00 ,  C12Q1/6869 ,  C12Q1/6869 ,  C12Q2563/159 ,  C12Q2565/629

Abstract: The present disclosure provides systems and methods for making a hydrogel comprising a cell, cell nucleus, or one or more components derived from a cell or cell nucleus. A method for making a hydrogel may comprise providing a cell or cell nucleus, a first polymer, wherein the first polymer comprises a plurality of first crosslink precursors, each of the plurality of first crosslink precursors comprising an azide group; providing a second polymer, wherein the second polymer comprises a plurality of second crosslink precursors, each of the plurality of second crosslink precursors comprising an alkyne group; and crosslinking the first polymer and the second polymer via a reaction between a first section of the first crosslink precursors and a second section of the second crosslink precursors, thereby providing the hydrogel comprising the cell or cell nucleus.