公开(公告)号：US20240264155A1

公开(公告)日：2024-08-08

申请号：US18429226

申请日：2024-01-31

Applicant: 10x Genomics, Inc.

Inventor： Justin COSTA

IPC: G01N33/543 ,  C12Q1/6844

CPC classification number: G01N33/54333 ,  C12Q1/6844 ,  C12Q2600/166

Abstract: The present disclosure relates in some aspects to methods and compositions for analyzing a biological sample. In some aspects, provided herein are detectably labeled magnetic particles, methods, and kits for analyzing a biological sample (e.g., for detecting a target nucleic acid at a location in the biological sample).

公开(公告)号：US20230374580A1

公开(公告)日：2023-11-23

申请号：US18320097

申请日：2023-05-18

Applicant: 10x Genomics, Inc.

Inventor： Justin COSTA

IPC: C12Q1/6865 ,  C12Q1/44 ,  C12Q1/6841

CPC classification number: C12Q1/6865 ,  C12Q1/44 ,  C12Q1/6841

Abstract: The present disclosure relates in some aspects to methods and compositions for analyzing a biological sample, comprising performing rolling circle amplification of a circRNA in situ in a biological sample, thereby generating an RCA product comprising multiple complementary copies of the circRNA and detecting the RCA product in situ at a spatially localized position in the biological sample.

公开(公告)号：US20230314328A1

公开(公告)日：2023-10-05

申请号：US18122093

申请日：2023-03-15

Applicant: 10x Genomics, Inc.

Inventor： Justin COSTA

IPC: G01N21/64

CPC classification number: G01N21/6458 ,  G01N2021/6471

Abstract: A branched flow imaging system capable of fluorescence microscopy is disclosed, the system including a light source configured to emit excitation light, a sample slide configured to hold a sample to be imaged, a medium providing a potential with a correlation length greater than the excitation light wavelength, and a sensor configured to capture fluorescence emission light emitted by the plurality of fluorophores of the sample. The medium may be positioned such that the excitation light branches into a plurality of light channels that are incident upon the sample slide so that each of the light channels randomly excites an individual fluorophore molecule of the sample. The degree of randomness of the distribution of the light channels on the sample may be determined by a difference between the correlation length of the potential and the wavelength of the excitation light.

公开(公告)号：US20220372570A1

公开(公告)日：2022-11-24

申请号：US17738538

申请日：2022-05-06

Applicant: 10x Genomics, Inc.

Inventor： Justin COSTA

IPC: C12Q1/6876 ,  C12Q1/682

Abstract: The present disclosure relates in some aspects to methods for analyzing a target nucleic acid in a biological sample. In some aspects, the methods involve the use of a set of oligonucleotides, for example a set of two or more oligonucleotides, wherein one or more oligonucleotides comprises one or more photoreactive nucleotides, for analyzing target nucleic acids. In some aspects, the presence, amount, and/or identity of a target nucleic acid is analyzed in situ. Also provided are oligonucleotides, sets of oligonucleotides, compositions, and kits for use in accordance with the methods.

公开(公告)号：US20220235403A1

公开(公告)日：2022-07-28

申请号：US17584222

申请日：2022-01-25

Applicant: 10x Genomics, Inc.

Inventor： Justin COSTA

IPC: C12Q1/6825 ,  C12Q1/6841

Abstract: The present disclosure relates in some aspects to proteins and nucleic acids for probing analytes in a biological sample. In some embodiments, disclosed herein is a method in which detection probes (e.g., fluorescently labeled detection probes) may comprise a sequence of nucleobases on a synthetic backbone, the detection probes therefore generating a greater signal than reference detection probes having the same sequence of nucleobases on a sugar-phosphate backbone.