公开(公告)号：US11247205B2

公开(公告)日：2022-02-15

申请号：US15953783

申请日：2018-04-16

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF THE UNIVERSITY OF ARIZONA

Inventor： Xuemin Wang ,  Christopher Baker ,  Craig A. Aspinwall

IPC: B01L3/00 ,  F16K99/00

Abstract: Surface-modified glass and polymer membrane interfaces form high-electrical resistance seals that can be used in microfluidic valves and array devices tailored for electrophysiological measurements. The incorporation of high seal resistance valves into the array device allows only the desired electrophysiological signal to be detected by a patch clamp amplifier, enabling parallel experiments with one patch clamp amplifier, which can greatly improve the cost efficiency. To achieve the desired high seal resistance, surface modification was performed on the glass components to increase the interaction between the glass and the membrane surfaces. The valves exhibit seal resistance of >500 GΩ after modification, which is 100× higher than reported for unmodified valves.

公开(公告)号：US20170120218A1

公开(公告)日：2017-05-04

申请号：US15318905

申请日：2015-06-30

Applicant: THE ARIZONA BOARD OF REGENTS ON BEHALF OF THE UNIVERSITY OF ARIZONA

Inventor： Steven Scott Saavedra ,  Craig A. Aspinwall ,  Saliya N. Ratnayaka ,  Elyssia S. Gallagher ,  Leonard Bright

IPC: B01J20/32 ,  C08F30/08 ,  B01J20/288

Abstract: Direct polymerization of lipid monomers or polymer scaffolding of non-lipid monomers coupled with irradiation or redox polymerization performed at neutral pH resulted in stabilized lipid assemblies. An initiator-buffer component and NaHS03 redox mixture polymerizes reactive lipid monomers at near neutral pH conditions to preserve functionality of reconstituted membrane proteins. Improved stability of black lipid membranes (BLMs) is attained by chemical cross-linking of polymerizable, hydrophobic and commercially available non-lipid monomers partitioned into the suspended lipid membranes, and by suspending the BLMs across low surface energy apertures. Substrate apertures having low surface energy modifiers with amphiphobic properties facilitated a reproducible formation of BLMs by promoting interactions between the lipid tail and the substrate material. In addition, polymeric lipid bilayer membranes were prepared by photochemical or redox initiated polymerization of polymerizable lipid monomers, and disposed onto supporting substrates for use in chromatography columns.