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公开(公告)号：US20180297029A1
公开(公告)日：2018-10-18
申请号：US15953783
申请日：2018-04-16
Inventor： Xuemin Wang , Christopher Baker , Craig A. Aspinwall
Abstract: Surface-modified glass and polymer membrane interfaces form high-electrical resistance seals that can be used in microfluidic valves and array devices tailored for electrophysiological measurements. The incorporation of high seal resistance valves into the array device allows only the desired electrophysiological signal to be detected by a patch clamp amplifier, enabling parallel experiments with one patch clamp amplifier, which can greatly improve the cost efficiency. To achieve the desired high seal resistance, surface modification was performed on the glass components to increase the interaction between the glass and the membrane surfaces. The valves exhibit seal resistance of >500GΩ after modification, which is 100× higher than reported for unmodified valves.
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公开(公告)号：US20180327833A1
公开(公告)日：2018-11-15
申请号：US15979185
申请日：2018-05-14
Inventor： Craig A. Aspinwall , Phuong-Diem Nguyen , Jinyan Wang , Xuemin Wang
IPC: C12Q1/6874 , C12Q1/6809 , C12Q1/6816 , C12Q1/6832 , C12Q1/6837
CPC classification number: C12Q1/6874 , C12Q1/6809 , C12Q1/6816 , C12Q1/6832 , C12Q1/6837
Abstract: Microarray platforms and methods of fabricating said microarrays without traditional high aspect ratio barriers used to define individual array elements are described herein. Self-assembled nanoshells were stabilized with a polymerized scaffold to enhance the stability in physiological conditions and serve as an optical transducer upon molecular recognition events. Soft photolithography combined with surface chemistry was developed for covalent immobilization of nanoshells onto the pre-patterned arrayed microspots for rapid multiplexed detection of membrane-binding analytes. This robust fabrication methodology is amenable for general lipid structures, and thus facilitates the integration of stable membrane architectures into diagnostic and prognostic platforms. In particular, the microarray platform may be used in diverse applications ranging from the detection of pathogens, such bacterial toxin in biological matrices, to cellular membrane studies.
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公开(公告)号：US11247205B2
公开(公告)日：2022-02-15
申请号：US15953783
申请日：2018-04-16
Inventor： Xuemin Wang , Christopher Baker , Craig A. Aspinwall
Abstract: Surface-modified glass and polymer membrane interfaces form high-electrical resistance seals that can be used in microfluidic valves and array devices tailored for electrophysiological measurements. The incorporation of high seal resistance valves into the array device allows only the desired electrophysiological signal to be detected by a patch clamp amplifier, enabling parallel experiments with one patch clamp amplifier, which can greatly improve the cost efficiency. To achieve the desired high seal resistance, surface modification was performed on the glass components to increase the interaction between the glass and the membrane surfaces. The valves exhibit seal resistance of >500 GΩ after modification, which is 100× higher than reported for unmodified valves.
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