公开(公告)号：US20210341401A1

公开(公告)日：2021-11-04

申请号：US17347938

申请日：2021-06-15

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF THE UNIVERSITY OF ARIZONA

Inventor： Craig A. Aspinwall ,  Colleen M. Janczak ,  Zeinab Mokhtari ,  Isen Andrew C. Calderon

IPC: G01N23/2202 ,  C09K11/02 ,  C09K11/06 ,  G01N33/573

Abstract: Scintillant-doped polystyrene core nanoparticles surrounded by a silica shell can be used to quantify low-energy radionuclides. The nanoparticles are recoverable and re-useable, which may reduce waste and allow for sample recovery. Unlike traditional liquid scintillation cocktail (LSC) formulations, the nanoparticles are made from non-toxic and non-volatile components, and can be used without the aid of surfactants, making them a possible alternative to LSC for reducing the environmental impact of studies that employ radioactive tracers. Recognition elements attached to the functionalized silica surfaces of the nanoparticles allow for separation-free scintillation proximity assay (SPA) applications in aqueous samples. Lipid membrane coatings deposited on the nanoparticle surface can significantly reduce the non-specific adsorption of proteins and other biomolecules, and allow for the incorporation of membrane proteins or other membrane associated binding molecules.

公开(公告)号：US20180327833A1

公开(公告)日：2018-11-15

申请号：US15979185

申请日：2018-05-14

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF THE UNIVERSITY OF ARIZONA

Inventor： Craig A. Aspinwall ,  Phuong-Diem Nguyen ,  Jinyan Wang ,  Xuemin Wang

IPC: C12Q1/6874 ,  C12Q1/6809 ,  C12Q1/6816 ,  C12Q1/6832 ,  C12Q1/6837

CPC classification number: C12Q1/6874 ,  C12Q1/6809 ,  C12Q1/6816 ,  C12Q1/6832 ,  C12Q1/6837

Abstract: Microarray platforms and methods of fabricating said microarrays without traditional high aspect ratio barriers used to define individual array elements are described herein. Self-assembled nanoshells were stabilized with a polymerized scaffold to enhance the stability in physiological conditions and serve as an optical transducer upon molecular recognition events. Soft photolithography combined with surface chemistry was developed for covalent immobilization of nanoshells onto the pre-patterned arrayed microspots for rapid multiplexed detection of membrane-binding analytes. This robust fabrication methodology is amenable for general lipid structures, and thus facilitates the integration of stable membrane architectures into diagnostic and prognostic platforms. In particular, the microarray platform may be used in diverse applications ranging from the detection of pathogens, such bacterial toxin in biological matrices, to cellular membrane studies.

公开(公告)号：US20220381789A1

公开(公告)日：2022-12-01

申请号：US17315688

申请日：2021-05-10

Applicant: Arizona Board of Regents on Behalf of the University of Arizona, a body corporate

Inventor： Craig A. Aspinwall ,  Zeinab Mohktari ,  Jon T. Njardarson ,  Colleen M. Janczak ,  Kevin Scott

IPC: G01N33/66

Abstract: The present invention provides compositions and methods for determining a saccharide level in a sample. In particular, compositions and methods of the invention include a boronic acid moiety that forms a complex with a saccharide.

公开(公告)号：US20180224438A1

公开(公告)日：2018-08-09

申请号：US15751125

申请日：2016-08-09

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF THE UNIVERSITY OF ARIZONA

Inventor： Craig A. Aspinwall ,  Jinyan Wang ,  Kendall Sandy ,  Steven Scott Saavedra ,  Christopher Baker ,  Elyssia S. Gallagher

IPC: G01N33/543 ,  G01N33/545 ,  G01N33/531 ,  B01L7/00 ,  B01L3/00

CPC classification number: G01N33/5432 ,  B01J20/286 ,  B01J20/3204 ,  B01J20/3272 ,  B01J20/3287 ,  B01J2220/84 ,  B01J2220/86 ,  B01L3/5082 ,  B01L7/00 ,  B01L2300/0832 ,  B01L2300/18 ,  B01L2300/1805 ,  C07K14/705 ,  C07K17/14 ,  G01N33/531 ,  G01N33/54353 ,  G01N33/545

Abstract: Surface-modified silica microparticles that are functionalized with stabilized phospholipid vesicles are described herein. These stabilized vesicles can be functionalized with either transmembrane receptors or membrane associated receptors and used for affinity pull-down assays or other chromatographic separation modalities to provide affinity capture/concentration of low abundance ligands in complex mixtures with minimal sample preparation. Further described are methods and apparatus for forming polymer frits in a fused silica capillary. The capillary containing a monomer solution is placed between one or more heat sources connected to each other via a jig and operatively coupled to a temperature controller. The polymer frits are synthesized via thermal polymerization of the monomer solution using the heat sources, which allows for placement of the polymer frits at a spatially-defined location in the capillary.

公开(公告)号：US10576456B2

公开(公告)日：2020-03-03

申请号：US15318905

申请日：2015-06-30

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF THE UNIVERSITY OF ARIZONA

Inventor： Steven Scott Saavedra ,  Craig A. Aspinwall ,  Saliya N. Ratnayaka ,  Leonard Bright

IPC: B01J20/32 ,  B01D69/10 ,  B32B3/24 ,  G01N33/487 ,  B82Y5/00 ,  B82Y15/00 ,  C08F30/08 ,  B01J20/288 ,  C08F2/48 ,  B01J20/286 ,  B82Y40/00 ,  B32B3/26 ,  G01N30/52 ,  B32B3/10

Abstract: Direct polymerization of lipid monomers or polymer scaffolding of non-lipid monomers coupled with irradiation or redox polymerization performed at neutral pH resulted in stabilized lipid assemblies. An initiator-buffer component and NaHS03 redox mixture polymerizes reactive lipid monomers at near neutral pH conditions to preserve functionality of reconstituted membrane proteins. Improved stability of black lipid membranes (BLMs) is attained by chemical cross-linking of polymerizable, hydrophobic and commercially available non-lipid monomers partitioned into the suspended lipid membranes, and by suspending the BLMs across low surface energy apertures. Substrate apertures having low surface energy modifiers with amphiphobic properties facilitated a reproducible formation of BLMs by promoting interactions between the lipid tail and the substrate material. In addition, polymeric lipid bilayer membranes were prepared by photochemical or redox initiated polymerization of polymerizable lipid monomers, and disposed onto supporting substrates for use in chromatography columns.

公开(公告)号：US20180297029A1

公开(公告)日：2018-10-18

申请号：US15953783

申请日：2018-04-16

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF THE UNIVERSITY OF ARIZONA

Inventor： Xuemin Wang ,  Christopher Baker ,  Craig A. Aspinwall

IPC: B01L3/00 ,  F16K99/00

Abstract: Surface-modified glass and polymer membrane interfaces form high-electrical resistance seals that can be used in microfluidic valves and array devices tailored for electrophysiological measurements. The incorporation of high seal resistance valves into the array device allows only the desired electrophysiological signal to be detected by a patch clamp amplifier, enabling parallel experiments with one patch clamp amplifier, which can greatly improve the cost efficiency. To achieve the desired high seal resistance, surface modification was performed on the glass components to increase the interaction between the glass and the membrane surfaces. The valves exhibit seal resistance of >500GΩ after modification, which is 100× higher than reported for unmodified valves.

公开(公告)号：US20210088509A1

公开(公告)日：2021-03-25

申请号：US17111037

申请日：2020-12-03

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF THE UNIVERSITY OF ARIZONA

Inventor： Craig A. Aspinwall ,  Jinyan Wang ,  Kendall Sandy ,  Steven Scott Saavedra ,  Christopher Baker ,  Elyssia S. Gallagher ,  Boying Liang

IPC: G01N33/543

Abstract: Surface-modified silica microparticles that are functionalized with stabilized phospholipid vesicles are described herein. These stabilized vesicles can be functionalized with either transmembrane receptors or membrane associated receptors and used for affinity pull-down assays or other chromatographic separation modalities to provide affinity capture/concentration of low abundance ligands in complex mixtures with minimal sample preparation. Further described are methods and apparatus for forming polymer frits in a fused silica capillary. The capillary containing a monomer solution is placed between one or more heat sources connected to each other via a jig and operatively coupled to a temperature controller. The polymer frits are synthesized via thermal polymerization of the monomer solution using the heat sources, which allows for placement of the polymer frits at a spatially-defined location in the capillary.

公开(公告)号：US20180118916A1

公开(公告)日：2018-05-03

申请号：US15798183

申请日：2017-10-30

Applicant: Arizona Board of Regents on Behalf of the University of Arizona

Inventor： Craig A. Aspinwall ,  Colleen M. Janczak ,  Zeinab Mokhtari ,  Isen Andrew C. Calderon

IPC: C08K5/00 ,  C08L25/06 ,  C08L83/02 ,  C08K5/353

CPC classification number: C08K5/0041 ,  C08K5/353 ,  C08L25/06 ,  C08L83/02 ,  C08L2207/53 ,  C09K11/00 ,  G01T1/16 ,  G01T1/20

Abstract: Scintillant-doped polystyrene core nanoparticles surrounded by a silica shell can be used to quantify low-energy radionuclides. The nanoparticles are recoverable and re-useable, which may reduce waste and allow for sample recovery. Unlike traditional liquid scintillation cocktail (LSC) formulations, the nanoparticles are made from non-toxic and non-volatile components, and can be used without the aid of surfactants, making them a possible alternative to LSC for reducing the environmental impact of studies that employ radioactive tracers. Recognition elements attached to the functionalized silica surfaces of the nanoparticles allow for separation-free scintillation proximity assay (SPA) applications in aqueous samples. Lipid membrane coatings deposited on the nanoparticle surface can significantly reduce the non-specific adsorption of proteins and other biomolecules, and allow for the incorporation of membrane proteins or other membrane associated binding molecules.

公开(公告)号：US20250062109A1

公开(公告)日：2025-02-20

申请号：US18720500

申请日：2022-12-14

Applicant: Arizona Board of Regents on Behalf of The University of Arizona

Inventor： Michael T. Marty ,  Craig A. Aspinwall ,  Marius M. Kostelic

IPC: H01J49/16 ,  C03C17/00 ,  C03C17/06 ,  C03C17/30 ,  G01N30/72

Abstract: An electrospray needle comprising a coating inside the needle, wherein the coating is a non-conductive and non-ionizable organic material, and in some embodiments comprising a conductive outer coating; methods of making the electrospray needle, systems that use the electrospray needle, methods of detection using the electrospray needle, and a kit that includes the electrospray needle.

公开(公告)号：US20220404344A1

公开(公告)日：2022-12-22

申请号：US17777011

申请日：2020-11-15

Applicant: Arizona Board of Regents on Behalf of the University of Arizona

Inventor： Craig A. Aspinwall ,  Minhui Han ,  Colleen Janczak

IPC: G01N33/547 ,  G01N33/533

Abstract: The present invention provides silica shell encapsulated polyaromatic-core microparticles and methods for producing and using the same. In particular, the silica shell encapsulated polyaromatic-core microparticles of the invention are hydrophilic microparticle scintillators comprising (i) polyaromatic-core microparticle (1), wherein said polyaromatic-core microparticle (1) is doped with a scintillator material (2); and (ii) a silica-shell portion (3) encapsulating said polyaromatic-core microparticle (1), wherein said silica-shell portion (3) comprises an outer surface (4). The polyaromatic-core portion is formed from an aromatic vinyl compound selected from the group consisting of styrene, vinyl toluene, and a mixture thereof.