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    Bacillus thuringiensis cry gene and protein
    11.
    发明授权
    Bacillus thuringiensis cry gene and protein 失效
    苏云金芽孢杆菌cry基因和蛋白质

    公开(公告)号：US07476781B2

    公开(公告)日：2009-01-13

    申请号：US11692973

    申请日：2007-03-29

    申请人： Andre R. Abad ,  Ronald D. Flannagan ,  Billy F. McCutchen ,  Cao Guo Yu

    发明人： Andre R. Abad ,  Ronald D. Flannagan ,  Billy F. McCutchen ,  Cao Guo Yu

    IPC分类号： A01H5/00 ,  A01H5/10 ,  C12N15/82

    CPC分类号： C07K14/325 ,  C12N15/8286 ,  Y02A40/162

    摘要： Compositions and methods for protecting a plant from an insect pest are provided. In particular, novel polynucleotides and the pesticidal polypeptides they encode are provided. Methods of using the novel polynucleotides and pesticidal polypeptides of the invention to protect a plant from an insect pest are further provided. Particular embodiments of the invention provide pesticidal compositions and formulations, DNA constructs, and transformed plants, plant cells, and seeds.

    摘要翻译： 提供了保护植物免受害虫害虫的组合物和方法。 特别地，提供了新型多核苷酸及其编码的杀虫多肽。 还提供了使用本发明的新型多核苷酸和杀虫多肽保护植物免受害虫的方法。 本发明的具体实施方案提供杀虫组合物和制剂，DNA构建体和转化的植物，植物细胞和种子。

    Bacillus thuringiensis cry gene and protein
    14.
    发明授权
    Bacillus thuringiensis cry gene and protein 有权
    苏云金芽孢杆菌cry基因和蛋白质

    公开(公告)号：US07329736B2

    公开(公告)日：2008-02-12

    申请号：US11404638

    申请日：2006-04-14

    申请人： Andre R. Abad ,  Ronald D. Flannagan ,  Billy F. McCutchen ,  Cao Guo Yu

    发明人： Andre R. Abad ,  Ronald D. Flannagan ,  Billy F. McCutchen ,  Cao Guo Yu

    IPC分类号： C07K14/415 ,  C07H21/04

    CPC分类号： C07K14/325 ,  C12N15/8286 ,  Y02A40/162

    摘要： Compositions and methods for protecting a plant from an insect pest are provided. In particular, novel polynucleotides and the pesticidal polypeptides they encode are provided. Methods of using the novel polynucleotides and pesticidal polypeptides of the invention to protect a plant from an insect pest are further provided. Particular embodiments of the invention provide pesticidal compositions and formulations, DNA constructs, and transformed plants, plant cells, and seeds.

    摘要翻译： 提供了保护植物免受害虫害虫的组合物和方法。 特别地，提供了新型多核苷酸及其编码的杀虫多肽。 还提供了使用本发明的新型多核苷酸和杀虫多肽保护植物免受害虫的方法。 本发明的具体实施方案提供杀虫组合物和制剂，DNA构建体和转化的植物，植物细胞和种子。

    Genes encoding novel Bacillus thuringiensis proteins with pesticidal activity against Coleopterans
    15.
    发明授权
    Genes encoding novel Bacillus thuringiensis proteins with pesticidal activity against Coleopterans 有权
    编码具有杀虫活性的新型苏云金芽孢杆菌蛋白的基因

    公开(公告)号：US07696412B2

    公开(公告)日：2010-04-13

    申请号：US10414637

    申请日：2003-04-16

    申请人： Andre R. Abad ,  Nicholas B. Duck ,  Xiang Feng ,  Ronald D. Flannagan ,  Theodore W. Kahn ,  Lynne E. Sims

    发明人： Andre R. Abad ,  Nicholas B. Duck ,  Xiang Feng ,  Ronald D. Flannagan ,  Theodore W. Kahn ,  Lynne E. Sims

    IPC分类号： A01H5/00 ,  A01H5/10 ,  C12N15/82 ,  C12N15/32

    CPC分类号： C07K14/325 ,  C12N15/8286 ,  Y02A40/162

    摘要： The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding δ-endotoxins having pesticidal activity against pests of the order Coleoptera. The invention further provides mutagenized nucleic acids that have been modified to encode endotoxins having improved pesticidal activity and/or altered pest specificity. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, expression cassettes comprising such nucleic acids, and transformed plants and seeds comprising a nucleic acid of the invention. These compositions find use in methods for controlling pests, especially plant pests.

    摘要翻译： 本发明提供从编号δ-内毒素的苏云金芽孢杆菌菌株获得的核酸及其变体和片段，其具有针对鞘翅目害虫的杀虫活性。 本发明还提供已被修饰以编码具有改善的杀虫活性和/或改变的有害生物特异性的内毒素的诱变的核酸。 本发明的具体实施方案提供编码杀虫蛋白的分离的核酸，包含此类核酸的表达盒，以及包含本发明核酸的转化植物和种子。 这些组合物可用于防治害虫，特别是植物害虫的方法。

    Bacillus thuringiensis cry9 nucleic acids
    16.
    发明授权
    Bacillus thuringiensis cry9 nucleic acids 有权
    苏云金芽孢杆菌cry9核酸

    公开(公告)号：US07629504B2

    公开(公告)日：2009-12-08

    申请号：US11018615

    申请日：2004-12-21

    申请人： Ronald D. Flannagan ,  Andre R. Abad

    发明人： Ronald D. Flannagan ,  Andre R. Abad

    IPC分类号： A01H5/00 ,  A01H5/10 ,  C12N15/32 ,  C12N15/82

    CPC分类号： A01N63/02 ,  C07K14/325 ,  C12N15/8286 ,  Y02A40/162

    摘要： The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding Cry9 δ-endotoxins having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, expression cassettes, and transformed plants comprising a nucleic acid of the invention.

    摘要翻译： 本发明提供了从编号Cry9δ-内毒素的苏云金芽孢杆菌菌株获得的核酸及其变体和片段，其对昆虫害虫包括鳞翅目具有杀虫活性。 本发明的具体实施方案提供编码杀虫蛋白，表达盒和包含本发明的核酸的转化植物的分离的核酸。

    Nucleic acids encoding Cry8Bb1 endotoxins engineered to have insect-specific protease recognition sequences
    17.
    发明授权
    Nucleic acids encoding Cry8Bb1 endotoxins engineered to have insect-specific protease recognition sequences 有权
    编码Cry8Bb1内毒素的核酸被改造为具有昆虫特异性蛋白酶识别序列

    公开(公告)号：US07473821B2

    公开(公告)日：2009-01-06

    申请号：US11448266

    申请日：2006-06-07

    申请人： Andre R. Abad ,  Gary C. Chun ,  Ronald D. Flannagan ,  Rafael Herrmann ,  Albert L. Lu ,  Janet A. Rice ,  Eric J. Schepers

    发明人： Andre R. Abad ,  Gary C. Chun ,  Ronald D. Flannagan ,  Rafael Herrmann ,  Albert L. Lu ,  Janet A. Rice ,  Eric J. Schepers

    IPC分类号： A01H5/00 ,  A01H5/10 ,  C12N15/82 ,  C12N15/32

    CPC分类号： C12N15/8286 ,  A01N63/02 ,  C07K14/325 ,  C12N9/6472 ,  Y02A40/162

    摘要： Compositions and methods for protecting a plant from an insect pest are provided. Nucleic acid molecules encoding insect protoxins or insect toxins modified to comprise at least one proteolytic activation site that is sensitive to an insect gut protease are provided. Cleavage of a modified insect protoxin at the proteolytic activation site by an insect gut protease produces an active insect toxin in the gut of the insect pest. Cleavage of a modified insect toxin of the invention at a proteolytic activation site results in the production of an active insect toxin in the insect gut that displays improved pesticidal activity relative to the insect toxin that lacks the proteolytic activation site. Methods of using the modified insect protoxin and modified insect toxin nucleic acid sequences and the polypeptides they encode to protect a plant from an insect pest are provided. Particular embodiments of the invention further provide modified insect protoxin and modified insect toxin compositions and formulations, expression cassettes, and transformed plants, plant cells, and seeds. Insect gut proteases and the nucleic acid molecules that encode them are also disclosed herein.

    摘要翻译： 提供了保护植物免受害虫害虫的组合物和方法。 提供编码昆虫原毒素或昆虫毒素的核酸分子，其被修饰为包含至少一个对昆虫肠道蛋白酶敏感的蛋白水解活化位点。 通过昆虫肠蛋白酶在蛋白水解活化位点切割修饰的昆虫原毒素在害虫的肠道中产生活性昆虫毒素。 在蛋白水解活化位点切割本发明的修饰的昆虫毒素导致在昆虫肠中产生活性昆虫毒素，其相对于缺乏蛋白水解活化位点的昆虫毒素显示出改善的杀虫活性。 提供了使用修饰的昆虫原毒素和修饰的昆虫毒素核酸序列及其编码的多肽以保护植物免受害虫的方法。 本发明的具体实施方案还提供改性的昆虫原毒素和修饰的昆虫毒素组合物和制剂，表达盒，以及转化的植物，植物细胞和种子。 昆虫肠道蛋白酶和编码它们的核酸分子也在本文中公开。

    Genes encoding novel proteins with pesticidal activity against coleopterans
    18.
    发明授权
    Genes encoding novel proteins with pesticidal activity against coleopterans 有权
    编码对鞘翅目具有杀虫活性的新型蛋白质的基因

    公开(公告)号：US08188036B2

    公开(公告)日：2012-05-29

    申请号：US12472997

    申请日：2009-05-27

    申请人： Andre R. Abad ,  Nicholas B. Duck ,  Xiang Feng ,  Ronald D. Flannagan ,  Theodore W. Kahn ,  Lynne E. Sims

    发明人： Andre R. Abad ,  Nicholas B. Duck ,  Xiang Feng ,  Ronald D. Flannagan ,  Theodore W. Kahn ,  Lynne E. Sims

    IPC分类号： A01N37/18

    CPC分类号： C07K14/325 ,  C12N15/8286 ,  Y02A40/162

    摘要： The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding δ-endotoxins having pesticidal activity against pests of the order Coleoptera. The invention further provides mutagenized nucleic acids that have been modified to encode endotoxins having improved pesticidal activity and/or altered pest specificity. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, expression cassettes, and transformed microorganisms and plants comprising a nucleic acid of the invention. These compositions find use in methods for controlling pests, especially plant pests.

    摘要翻译： 本发明提供从编号δ-内毒素的苏云金芽孢杆菌菌株获得的核酸及其变体和片段，其具有针对鞘翅目害虫的杀虫活性。 本发明还提供已被修饰以编码具有改善的杀虫活性和/或改变的有害生物特异性的内毒素的诱变的核酸。 本发明的具体实施方案提供编码杀虫蛋白，杀虫组合物，表达盒以及包含本发明核酸的转化微生物和植物的分离的核酸。 这些组合物可用于防治害虫，特别是植物害虫的方法。

    GENES ENCODING NOVEL PROTEINS WITH PESTICIDAL ACTIVITY AGAINST COLEOPTERANS
    19.
    发明申请
    GENES ENCODING NOVEL PROTEINS WITH PESTICIDAL ACTIVITY AGAINST COLEOPTERANS 有权
    基因编码具有杀虫活性的新型蛋白质

    公开(公告)号：US20090291896A1

    公开(公告)日：2009-11-26

    申请号：US12472997

    申请日：2009-05-27

    申请人： Andre R. Abad ,  Nicholas B. Duck ,  Xiang Feng ,  Ronald D. Flannagan ,  Theodore W. Kahn ,  Lynne E. Sims

    发明人： Andre R. Abad ,  Nicholas B. Duck ,  Xiang Feng ,  Ronald D. Flannagan ,  Theodore W. Kahn ,  Lynne E. Sims

    IPC分类号： A01N37/18 ,  C07K7/08 ,  C07K14/00 ,  A01P17/00

    CPC分类号： C07K14/325 ,  C12N15/8286 ,  Y02A40/162

    摘要： The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding δ-endotoxins having pesticidal activity against pests of the order Coleoptera. The invention further provides mutagenized nucleic acids that have been modified to encode endotoxins having improved pesticidal activity and/or altered pest specificity. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, expression cassettes, and transformed microorganisms and plants comprising a nucleic acid of the invention. These compositions find use in methods for controlling pests, especially plant pests.

    摘要翻译： 本发明提供从编码具有杀虫活性的针对鞘翅目害虫的具有杀虫活性的苏云金芽孢杆菌（Bacillus thuringiensis）菌株获得的核酸及其变体和片段。 本发明还提供已被修饰以编码具有改善的杀虫活性和/或改变的有害生物特异性的内毒素的诱变的核酸。 本发明的具体实施方案提供编码杀虫蛋白，杀虫组合物，表达盒以及包含本发明核酸的转化微生物和植物的分离的核酸。 这些组合物可用于防治害虫，特别是植物害虫的方法。

    Genes encoding novel bacillus thuringiensis proteins with pesticidal activity against coleopterans
    20.
    发明授权
    Genes encoding novel bacillus thuringiensis proteins with pesticidal activity against coleopterans 有权
    编码具有抗鞘翅目杀虫活性的新型苏云金芽孢杆菌蛋白的基因

    公开(公告)号：US07605304B2

    公开(公告)日：2009-10-20

    申请号：US10032717

    申请日：2001-10-23

    申请人： Andre R. Abad ,  Nicholas B. Duck ,  Xiang Feng ,  Ronald D. Flannagan ,  Theodore W. Kahn ,  Lynne E. Sims

    发明人： Andre R. Abad ,  Nicholas B. Duck ,  Xiang Feng ,  Ronald D. Flannagan ,  Theodore W. Kahn ,  Lynne E. Sims

    IPC分类号： A01H5/00 ,  A01H5/10 ,  C12N15/82 ,  C12N15/32

    CPC分类号： C07K14/325 ,  C12N15/8286 ,  Y02A40/162

    摘要： The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding δ-endotoxins having pesticidal activity against pests of the order Coleoptera. The invention further provides mutagenized nucleic acids that have been modified to encode endotoxins having improved pesticidal activity and/or altered pest specificity. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, expression cassettes comprising such nucleic acids, and transformed plants and seeds comprising a nucleic acid of the invention. These compositions find use in methods for controlling pests, especially plant pests.

    摘要翻译： 本发明提供从编码具有杀虫活性的针对鞘翅目害虫的具有杀虫活性的苏云金芽孢杆菌（Bacillus thuringiensis）菌株获得的核酸及其变体和片段。 本发明还提供已被修饰以编码具有改善的杀虫活性和/或改变的有害生物特异性的内毒素的诱变的核酸。 本发明的具体实施方案提供编码杀虫蛋白的分离的核酸，包含此类核酸的表达盒，以及包含本发明核酸的转化植物和种子。 这些组合物可用于防治害虫，特别是植物害虫的方法。