公开(公告)号：US20190331586A1

公开(公告)日：2019-10-31

申请号：US16390754

申请日：2019-04-22

Applicant: BECTON, DICKINSON AND COMPANY

Inventor： Joseph Trotter ,  Paul Barclay Purcell

IPC: G01N15/14 ,  B07C5/342

Abstract: Some embodiments of the methods provided herein relate to sample analysis and particle characterization methods. Some such embodiments include receiving, from a particle analyzer, measurements for a first portion of particles associated with an experiment. Some embodiments also include generating a tree representing groups of related particles based at least in part on the measurements, wherein the tree includes at least three groups. Some embodiments also include generating a measure of relatedness between a first group and a second group of the tree based at least in part on the measurements. Some embodiments also include and configuring the particle analyzer to classify a subsequent particle associated with the experiment with the first group real-time, wherein the subsequent particle is not included in the first portion of particles. Some embodiments also include sorting the subsequent particle.

公开(公告)号：US20140343897A1

公开(公告)日：2014-11-20

申请号：US14277527

申请日：2014-05-14

Applicant: BECTON, DICKINSON AND COMPANY

Inventor： Joseph Trotter ,  Christopher Wolf

IPC: G01N15/14

CPC classification number: G01N15/1429 ,  G01N2015/1006 ,  G01N2015/1402 ,  G06K9/0014 ,  G06K9/4638 ,  G06T7/12 ,  G06T7/194 ,  G06T9/20

Abstract: Methods and systems for efficient contour and gating in flow cytometry are provided. Event data is compressed to reduce the number of points needed to represent polygon contours for the event data. Selection of a level within the contour then causes the generation of a gate. This allows limited resource devices, such as touchscreen wireless devices, to render and gate flow cytometry data in a resource efficient manner.

Abstract translation: 提供流式细胞术中有效轮廓和门控的方法和系统。 事件数据被压缩以减少表示事件数据的多边形轮廓所需的点数。 轮廓内的水平的选择会导致门的产生。 这允许诸如触摸屏无线设备的有限的资源设备以资源有效的方式呈现和流量细胞计数数据。

公开(公告)号：US11227672B2

公开(公告)日：2022-01-18

申请号：US16596428

申请日：2019-10-08

Applicant: Becton, Dickinson and Company

Inventor： Jonathan Lin ,  Joseph Trotter ,  Keegan Owsley

IPC: G16C20/20 ,  G01N15/14 ,  G06K9/00 ,  B07C5/342 ,  B07C5/36

Abstract: A cell sorting system that automatically generates a sorting strategy based on examples of target events provided by an operator. The target events can be selected using measurements ranging from traditional flow cytometry measurements to derived measurements that are computationally expensive to complex measurements such as images.

公开(公告)号：US20190072478A1

公开(公告)日：2019-03-07

申请号：US16179646

申请日：2018-11-02

Applicant: Becton, Dickinson and Company

Inventor： Joseph Trotter ,  Sujata Iyer

IPC: G01N15/14

CPC classification number: G01N15/1459 ,  G01N15/1404 ,  G01N2015/1006 ,  G01N2015/149

Abstract: Methods and systems are disclosed for controlling a particle analyzer based at least in part on a generated entrainment factor for a sample. The features include flowing a sample with a series of particles through the particle analyzer, detecting events and calculating an expected frequency of those events based on a distribution, such as a Poisson distribution, and measuring an observed frequency of particle events. An entrainment factor may be generated from a ratio of observed event frequency to expected event frequency. Further adjustment to the particle analyzer maybe performed based on the indicated entrainment factor such as adjusted sorting bias.

公开(公告)号：US20190017921A1

公开(公告)日：2019-01-17

申请号：US16136605

申请日：2018-09-20

Applicant: Becton, Dickinson and Company

Inventor： Joseph Trotter ,  Christopher Wolf

IPC: G01N15/14 ,  G06T7/194 ,  G06T7/12 ,  G06T9/20 ,  G06K9/00

CPC classification number: G01N15/1429 ,  G01N2015/1006 ,  G01N2015/1402 ,  G06K9/0014 ,  G06K9/4638 ,  G06T7/12 ,  G06T7/194 ,  G06T9/20

Abstract: Methods and systems for efficient contour and gating in flow cytometry are provided. Event data is compressed to reduce the number of points needed to represent polygon contours for the event data. Selection of a level within the contour then causes the generation of a gate. This allows limited resource devices, such as touchscreen wireless devices, to render and gate flow cytometry data in a resource efficient manner.

公开(公告)号：US20140216128A1

公开(公告)日：2014-08-07

申请号：US14170409

申请日：2014-01-31

Applicant: BECTON DICKINSON AND COMPANY

Inventor： Joseph Trotter ,  Sujata Iyer

IPC: G01N15/00

CPC classification number: G01N15/1459 ,  G01N15/1404 ,  G01N2015/1006 ,  G01N2015/149

Abstract: Methods and systems are disclosed for generating an entrainment factor in a flow cytometry sample. The methods comprise flowing a sample with a series of particles through the flow cytometer, detecting events and calculating an expected frequency of those events based on a distribution, such as a Poisson distribution, and measuring an observed frequency of particle events. An entrainment factor may be generated from a ratio of observed event frequency to expected event frequency. Further adjustment to the flow cytometer maybe performed based on the indicated entrainment factor such as adjusted sorting bias.

Abstract translation: 公开了用于在流式细胞术样品中产生夹带因子的方法和系统。 所述方法包括使具有一系列颗粒的样品流过流式细胞仪，基于诸如泊松分布的分布来检测事件和计算那些事件的预期频率，以及测量观察到的粒子事件的频率。 可以从观察到的事件频率与预期事件频率的比率产生夹带因子。 可以基于所指示的夹带因子（例如经调整的分选偏差）进行对流式细胞仪的进一步调整。