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公开(公告)号：US11327003B2
公开(公告)日：2022-05-10
申请号：US16557539
申请日：2019-08-30
Applicant: BECTON, DICKINSON AND COMPANY
Inventor： Jonathan Lin , Joseph Trotter , Keegan Owsley
Abstract: Non-parametric transforms such as t-distributed stochastic neighbor embedding (tSNE) are used to analyze multi-parametric data such as data derived from flow cytometry or other particle analysis systems and methods. These transforms may be included for dimensionality reduction and identification of subpopulations (e.g., gating). By nature, non-parametric transforms cannot transform new observations without training a new transformation based on the entire dataset including the new observations. The features described parameterize non-parametric transforms using a neural network thereby allowing a small training dataset to be transformed using non-parametric techniques. The training dataset may then be used to generate an accurate parametric model for assessing additional events in a manner consistent with the initial events.
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公开(公告)号：US20210255087A1
公开(公告)日：2021-08-19
申请号：US17225567
申请日：2021-04-08
Applicant: Becton, Dickinson and Company
Inventor： Joseph Trotter , Paul Barclay Purcell
Abstract: Some embodiments of the methods provided herein relate to sample analysis and particle characterization methods. Some such embodiments include receiving, from a particle analyzer, measurements for a first portion of particles associated with an experiment. Some embodiments also include generating a tree representing groups of related particles based at least in part on the measurements, wherein the tree includes at least three groups. Some embodiments also include generating a measure of relatedness between a first group and a second group of the tree based at least in part on the measurements. Some embodiments also include and configuring the particle analyzer to classify a subsequent particle associated with the experiment with the first group real-time, wherein the subsequent particle is not included in the first portion of particles. Some embodiments also include sorting the subsequent particle.
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公开(公告)号：US10739248B2
公开(公告)日：2020-08-11
申请号：US16179646
申请日：2018-11-02
Applicant: Becton, Dickinson and Company
Inventor： Joseph Trotter , Sujata Iyer
Abstract: Methods and systems are disclosed for controlling a particle analyzer based at least in part on a generated entrainment factor for a sample. The features include flowing a sample with a series of particles through the particle analyzer, detecting events and calculating an expected frequency of those events based on a distribution, such as a Poisson distribution, and measuring an observed frequency of particle events. An entrainment factor may be generated from a ratio of observed event frequency to expected event frequency. Further adjustment to the particle analyzer maybe performed based on the indicated entrainment factor such as adjusted sorting bias.
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公开(公告)号：US10190963B2
公开(公告)日：2019-01-29
申请号：US14170409
申请日：2014-01-31
Applicant: Becton Dickinson and Company
Inventor： Joseph Trotter , Sujata Iyer
Abstract: Methods and systems are disclosed for generating an entrainment factor in a flow cytometry sample. The methods comprise flowing a sample with a series of particles through the flow cytometer, detecting events and calculating an expected frequency of those events based on a distribution, such as a Poisson distribution, and measuring an observed frequency of particle events. An entrainment factor may be generated from a ratio of observed event frequency to expected event frequency. Further adjustment to the flow cytometer maybe performed based on the indicated entrainment factor such as adjusted sorting bias.
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公开(公告)号：US10088407B2
公开(公告)日：2018-10-02
申请号：US14277527
申请日：2014-05-14
Applicant: BECTON, DICKINSON AND COMPANY
Inventor： Joseph Trotter , Christopher Wolf
Abstract: Methods and systems for efficient contour and gating in flow cytometry are provided. Event data is compressed to reduce the number of points needed to represent polygon contours for the event data. Selection of a level within the contour then causes the generation of a gate. This allows limited resource devices, such as touchscreen wireless devices, to render and gate flow cytometry data in a resource efficient manner.
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Patent Agency Ranking
公开(公告)号：US11644407B2
公开(公告)日：2023-05-09
申请号：US17545325
申请日：2021-12-08
Applicant: Becton, Dickinson and Company
Inventor： Jonathan Lin , Joseph Trotter , Keegan Owsley
CPC classification number: G16C20/20 , B07C5/3425 , B07C5/361 , G01N15/1425 , G01N15/1434 , G06V20/695 , G06V20/698 , G01N2015/149
Abstract: A cell sorting system that automatically generates a sorting strategy based on examples of target events provided by an operator. The target events can be selected using measurements ranging from traditional flow cytometry measurements to derived measurements that are computationally expensive to complex measurements such as images.
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公开(公告)号：US20220101955A1
公开(公告)日：2022-03-31
申请号：US17545325
申请日：2021-12-08
Applicant: Becton, Dickinson and Company
Inventor： Jonathan Lin , Joseph Trotter , Keegan Owsley
Abstract: A cell sorting system that automatically generates a sorting strategy based on examples of target events provided by an operator. The target events can be selected using measurements ranging from traditional flow cytometry measurements to derived measurements that are computationally expensive to complex measurements such as images.
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公开(公告)号：US10753848B2
公开(公告)日：2020-08-25
申请号：US16136605
申请日：2018-09-20
Applicant: Becton, Dickinson and Company
Inventor： Joseph Trotter , Christopher Wolf
Abstract: Methods and systems for efficient contour and gating in flow cytometry are provided. Event data is compressed to reduce the number of points needed to represent polygon contours for the event data. Selection of a level within the contour then causes the generation of a gate. This allows limited resource devices, such as touchscreen wireless devices, to render and gate flow cytometry data in a resource efficient manner.
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公开(公告)号：US11686663B2
公开(公告)日：2023-06-27
申请号：US17225567
申请日：2021-04-08
Applicant: Becton, Dickinson and Company
Inventor： Joseph Trotter , Paul Barclay Purcell
CPC classification number: G01N15/1429 , B07C5/3425 , G01N15/1425 , G01N2015/1006 , G01N2015/1081
Abstract: Some embodiments of the methods provided herein relate to sample analysis and particle characterization methods. Some such embodiments include receiving, from a particle analyzer, measurements for a first portion of particles associated with an experiment. Some embodiments also include generating a tree representing groups of related particles based at least in part on the measurements, wherein the tree includes at least three groups. Some embodiments also include generating a measure of relatedness between a first group and a second group of the tree based at least in part on the measurements. Some embodiments also include and configuring the particle analyzer to classify a subsequent particle associated with the experiment with the first group real-time, wherein the subsequent particle is not included in the first portion of particles. Some embodiments also include sorting the subsequent particle.
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公开(公告)号：US11002658B2
公开(公告)日：2021-05-11
申请号：US16390754
申请日：2019-04-22
Applicant: BECTON, DICKINSON AND COMPANY
Inventor： Joseph Trotter , Paul Barclay Purcell
Abstract: Some embodiments of the methods provided herein relate to sample analysis and particle characterization methods. Some such embodiments include receiving, from a particle analyzer, measurements for a first portion of particles associated with an experiment. Some embodiments also include generating a tree representing groups of related particles based at least in part on the measurements, wherein the tree includes at least three groups. Some embodiments also include generating a measure of relatedness between a first group and a second group of the tree based at least in part on the measurements. Some embodiments also include and configuring the particle analyzer to classify a subsequent particle associated with the experiment with the first group real-time, wherein the subsequent particle is not included in the first portion of particles. Some embodiments also include sorting the subsequent particle.
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