公开(公告)号：US20130343149A1

公开(公告)日：2013-12-26

申请号：US13922635

申请日：2013-06-20

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Daniel N. Fox ,  Nathan Michael Gaskill-Fox

IPC: B01F13/00

CPC classification number: G01N15/14 ,  B01F3/088 ,  B01F13/0059 ,  B01F15/0441 ,  G01N15/1404 ,  G01N2015/1006 ,  G01N2015/1409 ,  G05D11/131

Abstract: Disclosed is a system that can mix deionized water and concentrated sheath fluid to provide sheath fluid in a flow cytometer system having a desired concentration. Flow rates are low, which substantially match the flow rate of sheath fluid through the nozzle, so that turbulence and air bubbles are not formed in the sheath fluid. The available deionized water is then used for back flushing and removal of sample cells and deposited salts from the sheath fluid.

Abstract translation: 公开了一种可以将去离子水和浓缩鞘液混合以在具有所需浓度的流式细胞仪系统中提供鞘液的系统。 流速低，这与通过喷嘴的鞘液的流速基本一致，从而在鞘液中不形成湍流和气泡。 然后可用的去离子水用于从皮肤液中进行反冲洗和去除样品池和沉积的盐。

公开(公告)号：US20130333765A1

公开(公告)日：2013-12-19

申请号：US13918156

申请日：2013-06-14

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Daniel N. Fox ,  Nathan Michael Gaskill-Fox

IPC: G01N1/28

CPC classification number: G01N15/1404 ,  G01N1/28 ,  G01N1/286 ,  G01N2015/0065 ,  G01N2015/1006 ,  G01N2015/1406 ,  G01N2015/1409 ,  Y10T137/0324 ,  Y10T137/7287 ,  Y10T137/7303 ,  Y10T137/85978

Abstract: Disclosed is a sheath delivery system that uses a continuous flow of sheath fluid into a pressurized internal reservoir that substantially matches the outflow of sheath fluid through the nozzle of a flow cytometer. A substantially constant level of the sheath fluid is maintained. If the sheath fluid level falls below a desired level, or goes above a desired level, a dampened control system is used to reach the desired level. In addition, air pressure in the pressurized internal container is controlled so that an external sheath container can be removed and refilled with additional sheath fluid without stopping the sheath delivery system 100. Differences in pressure are detected by a droplet camera, which measures the droplet breakoff point to determine the pressure of the sheath fluid in the nozzle.

Abstract translation: 公开了一种护套递送系统，其使用鞘流体连续流入加压的内部储存器，其基本上匹配鞘液通过流式细胞仪的喷嘴的流出。 保持基本恒定水平的鞘液。 如果护套液体液位低于期望水平或高于所需水平，则使用阻尼控制系统达到所需水平。 此外，加压的内部容器中的空气压力被控制，使得可以移除外部护套容器并且用另外的护套流体重新填充，而不停止护套输送系统100.压力的差异通过液滴照相机来检测，其测量液滴断裂 以确定喷嘴中鞘液的压力。