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    Multi-directional sorting with reduced contamination in a flow cytometer
    1.
    发明授权
    Multi-directional sorting with reduced contamination in a flow cytometer 有权
    在流式细胞仪中减少污染的多方位分选

    公开(公告)号：US09162229B2

    公开(公告)日：2015-10-20

    申请号：US13923480

    申请日：2013-06-21

    Applicant: Bio-Rad Laboratories, Inc.

    Inventor： Nathan Michael Gaskill-Fox ,  Daniel N. Fox ,  Rodney C. Harris

    IPC: G01N15/14 ,  B01L9/00 ,  B01L9/06 ,  G01N35/10

    CPC classification number: B01L9/52 ,  B01L9/06 ,  G01N15/14 ,  G01N35/1011 ,  G01N2015/149

    Abstract: Disclosed is a collection sled for collecting a waste droplet stream 122 in a waste stream catcher that is located below sample collectors. Contamination of collected samples is reduced by collecting the waste droplet stream at a position that is below the sample collectors. A waste trough is provided that gets progressively larger in a downward direction, which prevents backsplash of waste fluid from the collection cavity. Standard sample collector tubes, as well as 8-well strips and microscope slides can be used with the collection sled to collect sample particles.

    Abstract translation: 公开了一种用于在位于样品收集器下方的废物流捕集器中收集废液滴流122的收集筒。 通过在低于样品收集器的位置处收集废液滴流来减少收集的样品的污染。 提供了在向下方向逐渐变大的废物槽，其防止废液从收集腔中脱落。 标准样品收集器管以及8孔条和显微镜载玻片可与收集雪橇一起使用以收集样品颗粒。

    TWO STATION SAMPLE AND WASHING SYSTEM
    2.
    发明申请
    TWO STATION SAMPLE AND WASHING SYSTEM 有权
    两站式样品和洗涤系统

    公开(公告)号：US20130340795A1

    公开(公告)日：2013-12-26

    申请号：US13923148

    申请日：2013-06-20

    Applicant: Bio-Rad Laboratories, Inc.

    Inventor： Nathan Michael Gaskill-Fox ,  Daniel N. Fox ,  Rodney C. Harris

    IPC: G01N11/02

    CPC classification number: G01N11/02 ,  G01N15/1404 ,  G01N35/1004

    Abstract: Disclosed is a two station system for a flow cytometer that includes a sample station and a wash station. During washing, the user has access to the sample station to insert a new sample. This increases the efficiency of the workflow process. Rotary clamps are used to automatically clamp the sample station and wash station to the system. A low volume pressurized cavity is used to bring the pressure of the sample to a desired pressure, which further increases productivity of the system. A transparent body is provided in the sample station so a user can view the sample during the sampling process. A backwash process is used to clean the sample injection tube and the sample uptake tube. In addition, the wash station is designed to rinse the outer surface of the sample uptake tube.

    Abstract translation: 公开了一种用于流式细胞仪的两站系统，其包括采样站和洗涤站。 在洗涤期间，用户可以访问样品台以插入新的样品。 这提高了工作流程的效率。 旋转夹具用于自动将样品站和洗涤台夹在系统中。 使用低体积加压腔使样品的压力达到所需的压力，这进一步提高了系统的生产率。 在采样台中设置透明体，因此用户可以在采样过程中观察样品。 反冲洗过程用于清洗样品注射管和样品吸收管。 此外，洗涤台被设计成冲洗样品吸收管的外表面。

    Flow rate balanced, dynamically adjustable sheath delivery system for flow cytometry
    5.
    发明授权
    Flow rate balanced, dynamically adjustable sheath delivery system for flow cytometry 有权
    流量平衡，动态可调护套输送系统，用于流式细胞术

    公开(公告)号：US09551637B2

    公开(公告)日：2017-01-24

    申请号：US13918156

    申请日：2013-06-14

    Applicant: Bio-Rad Laboratories, Inc.

    Inventor： Daniel N. Fox ,  Nathan Michael Gaskill-Fox

    IPC: G01N1/28 ,  G01N15/14

    CPC classification number: G01N15/1404 ,  G01N1/28 ,  G01N1/286 ,  G01N2015/0065 ,  G01N2015/1006 ,  G01N2015/1406 ,  G01N2015/1409 ,  Y10T137/0324 ,  Y10T137/7287 ,  Y10T137/7303 ,  Y10T137/85978

    Abstract: Disclosed is a sheath delivery system that uses a continuous flow of sheath fluid into a pressurized internal reservoir that substantially matches the outflow of sheath fluid through the nozzle of a flow cytometer. A substantially constant level of the sheath fluid is maintained. If the sheath fluid level falls below a desired level, or goes above a desired level, a dampened control system is used to reach the desired level. In addition, air pressure in the pressurized internal container is controlled so that an external sheath container can be removed and refilled with additional sheath fluid without stopping the sheath delivery system 100. Differences in pressure are detected by a droplet camera, which measures the droplet breakoff point to determine the pressure of the sheath fluid in the nozzle.

    Abstract translation: 公开了一种护套递送系统，其使用鞘流体连续流入加压的内部储存器，其基本上匹配鞘液通过流式细胞仪的喷嘴的流出。 保持基本恒定水平的鞘液。 如果护套液体液位低于期望水平或高于所需水平，则使用阻尼控制系统达到所需水平。 此外，加压的内部容器中的空气压力被控制，使得可以移除外部护套容器并且用另外的护套流体重新填充，而不停止护套输送系统100.压力的差异通过液滴照相机来检测，其测量液滴断裂 以确定喷嘴中鞘液的压力。

    Condensed geometry nozzle for flow cytometry
    6.
    发明授权
    Condensed geometry nozzle for flow cytometry 有权
    用于流式细胞仪的冷凝几何喷嘴

    公开(公告)号：US08980200B2

    公开(公告)日：2015-03-17

    申请号：US13923622

    申请日：2013-06-21

    Applicant: Bio-Rad Laboratories, Inc.

    Inventor： Daniel N. Fox ,  Matthias J. G. Ottenberg ,  Kevin P. Raley ,  Nathan Michael Gaskill-Fox

    IPC: B01L99/00 ,  B01L3/00 ,  G01N15/14

    CPC classification number: B01L3/56 ,  G01N15/1404 ,  G01N2015/1409 ,  G01N2015/1413 ,  Y10T29/49826

    Abstract: Disclosed is a nozzle assembly that is compact in size and that uses plastic tubing as an injection needle. Standard plastic fittings are utilized, which are inexpensive and widely available. The nozzle assembly has a simple construction and can be easily assembled and disassembled in a few minutes by a user. Cleaning and/or replacement of parts is inexpensive. Plastic tubing can be used as an injection needle that has superior qualities over commonly used stainless steel injection needles. Flexure of the injection needle tubing is prevented because of the compact size of the nozzle cavity.

    Abstract translation: 公开了一种尺寸紧凑并且使用塑料管作为注射针的喷嘴组件。 使用标准的塑料配件，其便宜且广泛可用。 喷嘴组件具有简单的结构，并且可以在几分钟内由用户容易地组装和拆卸。 清洁和/或更换零件便宜。 塑料管可用作注射针，其具有比常用的不锈钢注射针优异的质量。 由于喷嘴腔的紧凑尺寸，防止注射针管弯曲。

    MULTI-DIRECTIONAL SORTING WITH REDUCED CONTAMINATION IN A FLOW CYTOMETER
    7.
    发明申请
    MULTI-DIRECTIONAL SORTING WITH REDUCED CONTAMINATION IN A FLOW CYTOMETER 有权
    多方向分拣在流量计中减少污染

    公开(公告)号：US20130340539A1

    公开(公告)日：2013-12-26

    申请号：US13923480

    申请日：2013-06-21

    Applicant: Bio-Rad Laboratories, Inc.

    Inventor： Nathan Michael Gaskill-Fox ,  Daniel N. Fox ,  Rodney C. Harris

    IPC: B01L9/00

    CPC classification number: B01L9/52 ,  B01L9/06 ,  G01N15/14 ,  G01N35/1011 ,  G01N2015/149

    Abstract: Disclosed is a collection sled for collecting a waste droplet stream 122 in a waste stream catcher that is located below sample collectors. Contamination of collected samples is reduced by collecting the waste droplet stream at a position that is below the sample collectors. A waste trough is provided that gets progressively larger in a downward direction, which prevents backsplash of waste fluid from the collection cavity. Standard sample collector tubes, as well as 8-well strips and microscope slides can be used with the collection sled to collect sample particles.

    Abstract translation: 公开了一种用于在位于样品收集器下方的废物流捕集器中收集废液滴流122的收集筒。 通过在低于样品收集器的位置处收集废液滴流来减少收集的样品的污染。 提供了在向下方向逐渐变大的废物槽，其防止废液从收集腔中脱落。 标准样品收集器管以及8孔条和显微镜载玻片可与收集雪橇一起使用以收集样品颗粒。

    Two station sample and washing system
    8.
    发明授权
    Two station sample and washing system 有权

    公开(公告)号：US09927342B2

    公开(公告)日：2018-03-27

    申请号：US13923148

    申请日：2013-06-20

    Applicant: Bio-Rad Laboratories, Inc.

    Inventor： Nathan Michael Gaskill-Fox ,  Daniel N. Fox ,  Rodney C. Harris

    IPC: G01N35/10 ,  G01N11/02 ,  G01N15/14

    CPC classification number: G01N11/02 ,  G01N15/1404 ,  G01N35/1004

    Abstract: Disclosed is a two station system for a flow cytometer that includes a sample station and a wash station. During washing, the user has access to the sample station to insert a new sample. This increases the efficiency of the workflow process. Rotary clamps are used to automatically clamp the sample station and wash station to the system. A low volume pressurized cavity is used to bring the pressure of the sample to a desired pressure, which further increases productivity of the system. A transparent body is provided in the sample station so a user can view the sample during the sampling process. A backwash process is used to clean the sample injection tube and the sample uptake tube. In addition, the wash station is designed to rinse the outer surface of the sample uptake tube.

    CONDENSED GEOMETRY NOZZLE FOR FLOW CYTOMETRY
    10.
    发明申请
    CONDENSED GEOMETRY NOZZLE FOR FLOW CYTOMETRY 有权
    用于流动细胞仪的冷凝几何喷嘴

    公开(公告)号：US20130343965A1

    公开(公告)日：2013-12-26

    申请号：US13923622

    申请日：2013-06-21

    Applicant: Bio-Rad Laboratories, Inc.

    Inventor： Daniel N. Fox ,  Matthias J.G. Ottenberg ,  Kevin P. Raley ,  Nathan Michael Gaskill-Fox

    IPC: B01L3/00

    CPC classification number: B01L3/56 ,  G01N15/1404 ,  G01N2015/1409 ,  G01N2015/1413 ,  Y10T29/49826

    Abstract: Disclosed is a nozzle assembly that is compact in size and that uses plastic tubing as an injection needle. Standard plastic fittings are utilized, which are inexpensive and widely available. The nozzle assembly has a simple construction and can be easily assembled and disassembled in a few minutes by a user. Cleaning and/or replacement of parts is inexpensive. Plastic tubing can be used as an injection needle that has superior qualities over commonly used stainless steel injection needles. Flexure of the injection needle tubing is prevented because of the compact size of the nozzle cavity.

    Abstract translation: 公开了一种尺寸紧凑并且使用塑料管作为注射针的喷嘴组件。 使用标准的塑料配件，其便宜且广泛可用。 喷嘴组件具有简单的结构，并且可以在几分钟内由用户容易地组装和拆卸。 清洁和/或更换零件便宜。 塑料管可用作注射针，其具有比常用的不锈钢注射针优异的质量。 由于喷嘴腔的紧凑尺寸，防止注射针管弯曲。

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