Method for retrieval of unknown flanking DNA sequence
    11.
    发明授权
    Method for retrieval of unknown flanking DNA sequence 失效
    检索未知侧翼DNA序列的方法

    公开(公告)号:US5411875A

    公开(公告)日:1995-05-02

    申请号:US786902

    申请日:1991-11-01

    申请人: Douglas H. Jones

    发明人: Douglas H. Jones

    IPC分类号: C12N15/10 C12Q1/68 C12P19/34

    摘要: A method that permits the highly specific PCR amplification of unknown DNA that flanks a known sequence region. In this method, known DNA is placed on the uncharacterized side of that specific sequence that contains the unknown DNA by a DNA polymerase mediated generation of a PCR template that is shaped like a pan with a handle. Generation of this template permits specific amplification of the segment of interest.

    摘要翻译: 允许对已知序列区域侧翼的未知DNA进行高度特异性PCR扩增的方法。 在该方法中,将已知的DNA通过DNA聚合酶介导的PCR模板的生成形成为具有手柄的锅形状而置于含有未知DNA的特定序列的未表征侧。 生成此模板可以对感兴趣区段进行特定的扩增。