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公开(公告)号：USD402651S
公开(公告)日：1998-12-15
申请号：US75082
申请日：1997-08-01
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2.发明授权Methods and kits for analysis of chromosomal rearrangements associated with leukemia 失效用于分析与白血病相关的染色体重排的方法和试剂盒公开(公告)号：US06368791B1
公开(公告)日：2002-04-09
申请号：US09026033
申请日：1998-02-19
IPC分类号： C12Q168
CPC分类号： C12Q1/686 , C12Q1/6886 , C12Q2531/125
摘要： The invention relates to kits and methods for panhandle PCR amplification of a region of DNA having an unknown nucleotide sequence, wherein the region flanks a region of a leukemia-associated gene having a known nucleotide sequence in a human patient. Amplification of an unknown region flanking a known region of a leukemia-associated gene permits identification of a translocation partner of the gene or identification of a duplicated sequence within the gene. The invention further relates to kits useful for performing the methods of the invention, to an isolated polynucleotide, and to primers derived from such an isolated polynucleotide.
摘要翻译： 本发明涉及用于对具有未知核苷酸序列的DNA区域进行泛杂PCR扩增的试剂盒和方法，其中所述区域侧翼于人患者中具有已知核苷酸序列的白血病相关基因的区域。 在白血病相关基因的已知区域侧翼的未知区域的扩增允许鉴定基因的易位配偶体或鉴定该基因内的重复序列。 本发明还涉及可用于实施本发明的方法，分离的多核苷酸的试剂盒和衍生自这种分离的多核苷酸的引物。
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公开(公告)号：US06599703B2
公开(公告)日：2003-07-29
申请号：US09788038
申请日：2001-02-16
申请人： Douglas H. Jones
发明人： Douglas H. Jones
IPC分类号： C12Q168
CPC分类号： C12Q1/6869 , C12Q1/6844 , C12Q1/6855 , C12Q2525/131 , C12Q2521/313
摘要： An iterative and regenerative method for sequencing DNA is described. This method sequences DNA in discrete intervals starting at one end of a double stranded DNA segment. This method overcomes problems inherent in other sequencing methods, including the need for gel resolution of DNA fragments and the generation of artifacts caused by single-stranded DNA secondary structures. A particular advantage of this invention is that it can create offset collections of DNA segments and sequence the segments in parallel to provide continuous sequence information over long intervals. This method is also suitable for automation and multiplex automation to sequence large sets of segments.
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公开(公告)号：US06258533B1
公开(公告)日：2001-07-10
申请号：US09035183
申请日：1998-03-05
申请人： Douglas H. Jones
发明人： Douglas H. Jones
IPC分类号： C12Q168
CPC分类号： C12Q1/6869 , C12Q1/683 , C12Q1/6844 , C12Q1/6855 , C12Q1/6874 , C12Q2531/113 , C12Q2525/191 , C12Q2521/313 , C12Q2525/131 , C12Q2537/149
摘要： An iterative and regenerative method for sequencing DNA is described. This method sequences DNA in discrete intervals starting at one end of a double stranded DNA segment. This method overcomes problems inherent in other sequencing methods, including the need for gel resolution of DNA fragments and the generation of artifacts caused by single-stranded DNA secondary structures. A particular advantage of this invention is that it can create offset collections of DNA segments and sequence the segments in parallel to provide continuous sequence information over long intervals. This method is also suitable for automation and multiplex automation to sequence large sets of segments.
摘要翻译： 描述了用于测序DNA的迭代和再生方法。 该方法以从双链DNA片段的一端开始的离散间隔来排序DNA。 该方法克服了其他测序方法中固有的问题，包括DNA片段的凝胶拆分和由单链DNA二级结构引起的假象的产生。 本发明的一个特别优点在于它可以创建DNA片段的偏移集合并且对片段进行并行排列以在长间隔上提供连续的序列信息。 这种方法也适用于自动化和多路复用自动化以对大集合段进行排序。
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公开(公告)号：US5470722A
公开(公告)日：1995-11-28
申请号：US58907
申请日：1993-05-06
申请人： Douglas H. Jones
发明人： Douglas H. Jones
CPC分类号： C07K14/4712 , C12Q1/6855
摘要： A method that permits the rapid amplification of unknown DNA that flanks a known site, such that one can walk into an uncharacterized region of DNA. In this method, human genomic DNA is restriction enzyme digested and then ligated to a 5' phosphorylated-oligonucleotide so that the 5' end of each strand of genomic DNA is extended and phosphorylated. The phosphorylated-oligonucleotide is constructed to render 5' end extensions that are complementary to the known sequence. Following denaturation and re-annealing under dilute conditions that promote intrastrand annealing and under high stringency, only those DNA strands containing the known sequence will form a stem-loop structure with a recessed and phosphorylated 5' end, rendering a substrate for a subsequent heat-stable ligation reaction to another oligonucleotide. This second oligonucleotide is complementary to the sequence immediately adjacent to the phosphorylated-oligonucleotide high stringency annealing site. The heat-stable ligation reaction appends a known sequence to the DNA segments containing the two known contiguous DNA sequences used for oligonucleotide annealing. This heat-stable ligation of known sequence permits the subsequent highly specific amplification of the unknown flanking DNA.
摘要翻译： 允许快速扩增位于已知位点侧翼的未知DNA的方法，使得人可以进入DNA的未表征区域。 在该方法中，人基因组DNA被限制酶消化，然后连接到5'磷酸化寡核苷酸，使得每条链基因组DNA的5'末端延伸并磷酸化。 构建磷酸化寡核苷酸以使得与已知序列互补的5'末端延伸。 在促进柱内退火和高严格性的稀释条件下变性和再退火之后，只有含有已知序列的那些DNA链将形成具有凹陷和磷酸化的5'端的茎 - 环结构，从而形成随后的热 - 与另一寡核苷酸的稳定连接反应。 该第二寡核苷酸与紧邻磷酸化寡核苷酸高度严格退火位点的序列互补。 热稳定连接反应将已知的序列附加到含有用于寡核苷酸退火的两个已知的连续DNA序列的DNA片段上。 已知序列的这种热稳定连接允许随后的未知侧翼DNA的高度特异性扩增。
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公开(公告)号：US5286632A
公开(公告)日：1994-02-15
申请号：US638512
申请日：1991-01-09
申请人： Douglas H. Jones
发明人： Douglas H. Jones
CPC分类号： C12Q1/6858 , C12N15/102 , C12N15/64 , C12Q1/6853
摘要： The subject invention relates to a method referred to as recombination PCR (RPCR). In the method, the polymerase chain reaction is utilized to add double-stranded homologous ends to DNA. These homologous ends undergo recombination in vivo following transfection of host cells. The placement of these homologous ends, by the amplifying primers permits the rapid cloning of the desired mutant or recombinant, with a minimal number of steps and primers.
摘要翻译： 本发明涉及称为重组PCR（RPCR）的方法。 在该方法中，利用聚合酶链反应将DNA双链同源末端加入。 这些同源末端在转染宿主细胞后在体内进行重组。 通过扩增引物放置这些同源末端允许用最少量的步骤和引物快速克隆所需的突变体或重组体。
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公开(公告)号：USD429253S
公开(公告)日：2000-08-08
申请号：US102147
申请日：1999-03-17
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公开(公告)号：US5858671A
公开(公告)日：1999-01-12
申请号：US742755
申请日：1996-11-01
申请人： Douglas H. Jones
发明人： Douglas H. Jones
CPC分类号： C12Q1/6869 , C12Q1/6844 , C12Q1/6855
摘要： An iterative and regenerative method for sequencing DNA is described. This method sequences DNA in discrete intervals starting at one end of a double stranded DNA segment. This method overcomes problems inherent in other sequencing methods, including the need for gel resolution of DNA fragments and the generation of artifacts caused by single-stranded DNA secondary structures. A particular advantage of this invention is that it can create offset collections of DNA segments and sequence the segments in parallel to provide continuous sequence information over long intervals. This method is also suitable for automation and multiplex automation to sequence large sets of segments.
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公开(公告)号：US5411875A
公开(公告)日：1995-05-02
申请号：US786902
申请日：1991-11-01
申请人： Douglas H. Jones
发明人： Douglas H. Jones
CPC分类号： C12N15/10 , C12Q1/6855 , Y10S435/81
摘要： A method that permits the highly specific PCR amplification of unknown DNA that flanks a known sequence region. In this method, known DNA is placed on the uncharacterized side of that specific sequence that contains the unknown DNA by a DNA polymerase mediated generation of a PCR template that is shaped like a pan with a handle. Generation of this template permits specific amplification of the segment of interest.
摘要翻译： 允许对已知序列区域侧翼的未知DNA进行高度特异性PCR扩增的方法。 在该方法中，将已知的DNA通过DNA聚合酶介导的PCR模板的生成形成为具有手柄的锅形状而置于含有未知DNA的特定序列的未表征侧。 生成此模板可以对感兴趣区段进行特定的扩增。
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公开(公告)号：USD278922S
公开(公告)日：1985-05-21
申请号：US447655
申请日：1982-12-07
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