公开(公告)号：US20130266480A1

公开(公告)日：2013-10-10

申请号：US13911872

申请日：2013-06-06

Applicant: FUJIFILM Corporation

Inventor： Toshihito KIMURA ,  Akihiro KOMATSU ,  Toshihiro MORI ,  Hideyuki KARAKI ,  Nobuhiko FUJIWARA ,  Kouta KATOU ,  Tadahiro MATSUNO

IPC: G01N21/64

CPC classification number: G01N21/6428 ,  B01L3/5027 ,  B01L2200/025 ,  B01L2400/0487 ,  G01N21/03 ,  G01N21/6408 ,  G01N21/648 ,  G01N33/54366 ,  G01N2021/0328

Abstract: An assay chip includes fluidic-channel member composed of a light-transmissive lower member and an upper member, forming a fluidic-channel therebetween, and a cover member fitted with the fluidic-channel member from the upper-member-side thereof. An inlet for injecting a sample solution into the fluidic-channel and a suction opening for sucking, from the downstream side, the injected sample solution, both communicating with the fluidic-channel, are formed on the upper surface of the upper member. A pot for carrying out predetermined pre-processing on the sample solution, a pot for first-reaction processing to bind a photoresponsive labeling substance to an analyte in the sample solution, an inlet insertion-hole for inserting the inlet, and a suction-opening insertion-hole for inserting the suction opening are linearly arranged on the upper surface of the cover member.

Abstract translation: 分析芯片包括由透光下部构件和上部构件构成的流体通道构件，在其间形成流体通道，以及从其上部构件侧装配有流体通道构件的盖构件。 用于将样品溶液注入流体通道的入口和用于从下游侧吸入与流体通道连通的注入的样品溶液的吸入口形成在上部构件的上表面上。 用于对样品溶液进行预定的预处理的罐，用于将光响应性标记物质结合到样品溶液中的分析物的第一反应处理的罐，用于插入入口的入口插入孔和吸入口 用于插入吸入口的插入孔直线地布置在盖构件的上表面上。

公开(公告)号：US20130224764A1

公开(公告)日：2013-08-29

申请号：US13854640

申请日：2013-04-01

Applicant: FUJIFILM Corporation

Inventor： Toshihito KIMURA

IPC: G01N21/64

CPC classification number: G01N21/6486 ,  G01N21/6428 ,  G01N21/6445 ,  G01N33/54373 ,  G01N2021/6439

Abstract: A biological molecule detecting apparatus capable of highly sensitive measurements is provided. The emission direction of an orientation controlling light beam was periodically switched, to periodically switch the orientation direction of binding molecules 15 within a solution. Components, which are synchronized with the orientation periods of the binding molecules are extracted from fluorescence emitted by fluorescent molecules within the solution, are extracted and detected. Thereby, the concentration of a detection target substance can be accurately measured with a simple configuration.

Abstract translation: 提供能够进行高灵敏度测量的生物分子检测装置。 定向切换定向控制光束的发射方向，以周期性地切换溶液内的结合分子15的取向方向。 与结合分子的取向周期同步的组分从溶液内的荧光分子发出的荧光中提取并提取并检测。 由此，能够以简单的结构精确地测定检测对象物质的浓度。

公开(公告)号：US20130224763A1

公开(公告)日：2013-08-29

申请号：US13854494

申请日：2013-04-01

Applicant: FUJIFILM CORPORATION

Inventor： Toshihito KIMURA

IPC: G01N21/64

CPC classification number: G01N21/6486 ,  G01N21/6428

Abstract: A biological molecule detecting apparatus capable of highly sensitive measurements is provided. A laser was emitted onto a solution, to impart external force onto free molecules and binding molecules within the solution. The external force inhibited Brownian motion of the free molecules and the binding molecules. The concentration of a detection target substance which is associated the binding molecules can be measured with high sensitivity, by measuring the Brownian motion of the free molecules and the binding molecules within the solution irradiated with the laser.

Abstract translation: 提供能够进行高灵敏度测量的生物分子检测装置。 将激光发射到溶液中，以将外力赋予自由分子并在溶液中结合分子。 外力抑制自由分子和结合分子的布朗运动。 可以通过测量自由分子的布朗运动和用激光照射的溶液中的结合分子，以高灵敏度测量与结合分子相关的检测目标物质的浓度。