公开(公告)号：US11198127B2

公开(公告)日：2021-12-14

申请号：US16348170

申请日：2017-11-08

Applicant: Georgia Tech Research Corporation

Inventor： Todd Sulchek ,  Alexander Alexeev ,  Anna Liu

IPC: B01L3/00 ,  C12M3/06 ,  C12M1/42 ,  C12M1/00 ,  C12N15/87 ,  C12N5/07

Abstract: Embodiments of the present disclosure can include a method for convective intracellular delivery including providing cells and molecules to a microchannel having compressive surfaces, wherein the compressive surfaces define compression gaps having a height of from 20 and 80% of the average cell diameter; and a plurality of relaxation spaces disposed between the compressive surfaces; flowing the cell medium through the microchannel, wherein as the cell medium flows through the microchannel, the plurality of cells undergo a convective intracellular delivery process comprising: compressing the plurality of cells, wherein the compressing causes the plurality of cells to undergo a loss in intracellular volume (Vloss); and passing the plurality of cells to a first relaxation space, wherein the plurality of cells undergo a gain in volume (Vgain) and absorb a portion of the plurality of molecules.

公开(公告)号：US10717084B2

公开(公告)日：2020-07-21

申请号：US15774684

申请日：2016-11-09

Applicant: GEORGIA TECH RESEARCH CORPORATION

Inventor： Todd Sulchek ,  Alexander Alexeev ,  Gonghao Wang

IPC: G01N33/48 ,  G01N33/00 ,  G01N15/06 ,  B01L3/00 ,  G01N15/02 ,  G01N33/50 ,  G01N15/00 ,  G01N33/49

Abstract: Microfluidic devices for cell sorting or cell fractionation are disclosed. A microfluidic device can comprise one or more inlets, a first wall and a second wall, and two or more outlets. The first and second walls can be substantially planar to each other and the first wall having can have a plurality of ridges protruding from the first wall and defining a compression gap between the ridge and a surface of the second wall. The microfluidic device can also be a cell sorting device for sorting a plurality of cells based on one or more biophysical cellular properties including size, elasticity, viscosity, and/or viscoelasticity wherein the cells are subjected to one or more compressions due to the compression gap. Also disclosed are methods for cell sorting based on a variety of biophysical cellular properties.

公开(公告)号：US20190262835A1

公开(公告)日：2019-08-29

申请号：US16348170

申请日：2017-11-08

Applicant: Georgia Tech Research Corporation

Inventor： Todd Sulchek ,  Alexander Alexeev ,  Anna Liu

IPC: B01L3/00 ,  C12M1/42 ,  C12M3/06 ,  C12M1/00 ,  C12N15/87

Abstract: Embodiments of the present disclosure can include a method for convective intracellular delivery including providing cells and molecules to a microchannel having compressive surfaces, wherein the compressive surfaces define compression gaps having a height of from 20 and 80% of the average cell diameter; and a plurality of relaxation spaces disposed between the compressive surfaces; flowing the cell medium through the microchannel, wherein as the cell medium flows through the microchannel, the plurality of cells undergo a convective intracellular delivery process comprising: compressing the plurality of cells, wherein the compressing causes the plurality of cells to undergo a loss in intracellular volume (Vloss); and passing the plurality of cells to a first relaxation space, wherein the plurality of cells undergo a gain in volume (Vgain) and absorb a portion of the plurality of molecules.