公开(公告)号：US20190127741A1

公开(公告)日：2019-05-02

申请号：US16143189

申请日：2018-09-26

Applicant: Genentech, Inc.

Inventor： Laetitia MALPHETTES ,  Andrew Snowden ,  Inn H. Yuk

IPC: C12N15/67 ,  C12N15/63

Abstract: The invention provides compositions and methods for regulating intracellular osmolarity in cells, e.g., in cultured cells, including in cultured cells in bioreactors. The invention provides nucleic acids comprising at least one osmo-responsive transcriptional regulatory element (OR-TRE), and cells, vectors, products of manufacture, artificial organs or implants and the like containing an osmo-responsive transcriptional regulatory element (OR-TRE).

公开(公告)号：US20180364235A1

公开(公告)日：2018-12-20

申请号：US15914094

申请日：2018-03-07

Applicant: Genentech, Inc.

Inventor： Feny Gunawan ,  Yi-Chun Hsiao ,  Denise C. Krawitz ,  Margaret S. Lin ,  Martin Vanderlaan ,  Rajesh Vij ,  Inn H. Yuk ,  Judith Zhu-Shimoni

IPC: G01N33/573 ,  G01N33/577 ,  C07K16/40

Abstract: Monoclonal and polyclonal antibodies that bind hamster phospholipase B-like 2 are provided. Also provided are methods for detecting and quantifying hamster phospholipase B-like 2, for example, in recombinant polypeptide preparations, as well as kits for carrying out such methods. Methods of screening or selecting host cell lines or recombinant polypeptide-expressing cell lines that express low levels of hamster phospholipase B-like 2 are also provided.