公开(公告)号：US20160222339A1

公开(公告)日：2016-08-04

申请号：US14917115

申请日：2013-09-09

Applicant: HITACHI, LTD.

Inventor： Yasuhiko TADA ,  Keisuke SHIBUYA ,  Yui SUGITA

IPC: C12M1/00 ,  C12M1/12 ,  C12N5/09 ,  C12N5/071 ,  C12N5/0781

CPC classification number: C12M47/04 ,  B01D15/3809 ,  B01D15/3876 ,  B01J20/24 ,  B01J20/28033 ,  B01J20/321 ,  B01J20/3219 ,  B01J20/3274 ,  B01J20/3278 ,  B01J20/3297 ,  C12M23/04 ,  C12M23/20 ,  C12M25/02 ,  C12M47/02 ,  C12N5/0635 ,  C12N5/0682 ,  C12N5/0693

Abstract: Provided are a cell separation and collection membrane capable of suppressing non-specific adsorption of cells while specifically adsorbing target cells; a culturing sheet and a culturing apparatus which use the membrane; and a cell separation and collection method. The cell separation and collection membrane included in the culturing sheet comprises: a support; a polymer site fixed to the support and having a changeable structure in accordance with a temperature; a cell adsorbing site bound to the polymer site, exposed relative to an outer surface of the support, and specifically adsorbing a target cell when the cell adsorbing site is brought into contact with a treatment solution containing the target cells; and a hydrophilic site exposed relative to the outer surface and to be brought into contact with the treatment solution.

Abstract translation: 提供能够抑制特异性吸附靶细胞的细胞非特异性吸附的细胞分离收集膜; 培养片和使用该膜的培养装置; 和细胞分离收集方法。 包含在培养片中的细胞分离收集膜包括：载体; 固定在载体上并具有根据温度的可变结构的聚合物部位; 与聚合物部位结合的细胞吸附部位相对于载体的外表面露出，并且当使细胞吸附部位与含有靶细胞的处理液接触时特异性吸附靶细胞; 亲水部位相对于外表面露出并与处理液接触。