公开(公告)号：US07449324B2

公开(公告)日：2008-11-11

申请号：US10371099

申请日：2003-02-21

申请人： Ronaldus Adrianus Maria Fouchier ,  Bernadetta Gerarda Van Den Hoogen ,  Albertus Dominicus Marcellinus Erasmus Osterhaus ,  Jan Cornelius De Jong ,  Jan Groen

发明人： Ronaldus Adrianus Maria Fouchier ,  Bernadetta Gerarda Van Den Hoogen ,  Albertus Dominicus Marcellinus Erasmus Osterhaus ,  Jan Cornelius De Jong ,  Jan Groen

IPC分类号： C12N7/02

CPC分类号： C12Q1/701 ,  A61K39/12 ,  A61K39/155 ,  A61K2039/5254 ,  A61K2039/5256 ,  A61K2039/545 ,  A61K2039/552 ,  A61K2039/58 ,  A61K2039/70 ,  A61K2123/00 ,  C07K14/005 ,  C07K16/1027 ,  C12N7/00 ,  C12N15/86 ,  C12N2710/22034 ,  C12N2760/18321 ,  C12N2760/18322 ,  C12N2760/18334 ,  C12N2760/18343 ,  C12N2760/18522 ,  C12N2760/18534 ,  C12N2760/18621 ,  C12N2760/18622 ,  C12N2760/18634 ,  C12N2760/18643 ,  C12N2840/203 ,  C12Q2600/158 ,  G01N33/5008 ,  G01N33/5011 ,  G01N33/502 ,  G01N33/5088 ,  G01N33/56983 ,  G01N2333/08 ,  Y02A90/26

摘要： The present invention provides an isolated mammalian negative strand RNA virus, metapneumovirus (MPV), within the sub-family Pneumoviridae, of the family Paramyxoviridae. The invention also provides isolated mammalian negative strand RNA viruses identifiable as phylogenetically corresponding or relating to the genus Metapneumovirus and components thereof. In particular the invention provides a mammalian MPV, subgroups and variants thereof. The invention relates to genomic nucleotide sequences of different isolates of mammalian metapneumoviruses, in particular human metapneumoviruses. The invention relates to the use of the sequence information of different isolates of mammalian metapneumoviruses for diagnostic and therapeutic methods. The present invention relates to nucleotide sequences encoding the genome of a metapneumovirus or a portion thereof, including both mammalian and avian metapneumovirus. The invention further encompasses chimeric or recombinant viruses encoded by said nucleotide sequences. The invention also relates to chimeric and recombinant mammalian MPV that comprise one or more non-native or heterologous sequences. The invention further relates to vaccine formulations comprising mammalian or avian metapneumovirus, including recombinant and chimeric forms of said viruses. The vaccine preparations of the invention encompass multivalent vaccines, including bivalent and trivalent vaccine preparations.

摘要翻译： 本发明提供分离的哺乳动物负链RNA病毒，亚型家族肺炎链球菌中的亚型副粘病毒亚科病毒（MPV）。 本发明还提供可分离的哺乳动物负链RNA病毒，其可鉴定为与肺炎病毒属及其组分相关或与之相关的系统发育。 特别地，本发明提供哺乳动物MPV，亚组及其变体。 本发明涉及哺乳动物偏肺炎病毒，特别是人类偏肺病毒的不同分离物的基因组核苷酸序列。 本发明涉及用于诊断和治疗方法的哺乳动物偏肺病毒的不同分离株的序列信息的使用。 本发明涉及编码偏肺病毒或其部分基因组的核苷酸序列，包括哺乳动物和禽类间质肺病毒。 本发明还包括由所述核苷酸序列编码的嵌合或重组病毒。 本发明还涉及包含一种或多种非天然或异源序列的嵌合和重组哺乳动物MPV。 本发明还涉及包含哺乳动物或禽类偏肺病毒的疫苗制剂，包括所述病毒的重组和嵌合形式。 本发明的疫苗制剂包括多价疫苗，包括二价和三价疫苗制剂。