公开(公告)号：US5686308A

公开(公告)日：1997-11-11

申请号：US488630

申请日：1995-06-08

申请人： Yi Li ,  Carole Young ,  Timothy J. Fischer ,  James H. Carter

发明人： Yi Li ,  Carole Young ,  Timothy J. Fischer ,  James H. Carter

IPC分类号： G01N33/48 ,  G01N33/49 ,  G01N33/50 ,  G01N33/569 ,  G01N33/72 ,  G01N31/00

CPC分类号： G01N33/5094 ,  G01N33/56972 ,  G01N33/721 ,  Y10T436/10 ,  Y10T436/101666 ,  Y10T436/107497 ,  Y10T436/108331

摘要： A lytic reagent composition is provided which selectively stromatolyses red blood cells in a blood sample. In addition, a lytic reagent system is provided which enable the differentiation of at least two subpopulations of leukocytes. A method for using the lytic reagent system is also provided. Still further, the lytic reagent system find use in the determination of the hemoglobin in the blood. The lytic reagent system utilizes an ethoxylated long chain compound based lysis reagent with an acid and a hypertonic, alkaline, stabilizing reagent. The system and analysis method maintains the cellular morphology of the leukocytes and can be used to analyze normal and abnormal blood samples, fresh and aged blood, human and non-human animal blood samples, as well as other fluid samples such as bone marrow.

摘要翻译： 提供溶解试剂组合物，其选择性地在血液样品中进行红细胞分解。 此外，提供了使得能够分化白细胞的至少两个亚群的溶解试剂系统。 还提供了使用溶解试剂系统的方法。 此外，溶解试剂系统可用于测定血液中的血红蛋白。 裂解试剂系统利用具有酸和高渗，碱性稳定剂的乙氧基化长链化合物裂解试剂。 系统和分析方法维持白细胞的细胞形态，可用于分析正常和异常血液样本，新鲜和老化血液，人和非人动物血液样品以及其他流体样品如骨髓。

公开(公告)号：US5849513A

公开(公告)日：1998-12-15

申请号：US904400

申请日：1997-07-31

申请人： Gerald E. Jaffe ,  Frank J. Lucas ,  James H. Carter

发明人： Gerald E. Jaffe ,  Frank J. Lucas ,  James H. Carter

IPC分类号： C12Q1/02 ,  C12Q1/34 ,  G01N33/574 ,  C12Q1/00

CPC分类号： C12Q1/34 ,  C12Q1/02 ,  G01N33/574 ,  C12Q2334/40 ,  C12Q2337/40

摘要： An assay compound or a salt thereof for assaying the activity of an enzyme inside a metabolically active whole cell is disclosed. The assay compound includes a leaving group and an indicator group. The leaving group is selected from the group comprising amino acids, peptides, saccharides, sulfates, phosphates, esters, phosphate esters, nucleotides, polynucleotides, nucleic acids, pyrimidines, purines, nucleosides, lipids and mixtures thereof. The indicator group is selected from compounds which have a first state when joined to the leaving group, and a second state when the leaving group is cleaved from the indicator group by the enzyme. Preferably, the indicator compounds are rhodamine 110, rhodol, and fluorescein and analogs of these compounds. A method of synthesizing the compound as well as methods of using these compounds to measure enzyme activity are also disclosed.

公开(公告)号：US5843608A

公开(公告)日：1998-12-01

申请号：US963784

申请日：1997-11-04

申请人： Yi Li ,  Carole Young ,  Timothy J. Fischer ,  James H. Carter ,  Sergio C. Veulens

发明人： Yi Li ,  Carole Young ,  Timothy J. Fischer ,  James H. Carter ,  Sergio C. Veulens

IPC分类号： G01N33/48 ,  G01N33/49 ,  G01N33/50 ,  G01N33/569 ,  G01N33/72 ,  G01N31/00

CPC分类号： G01N33/56972 ,  G01N33/5094 ,  G01N33/721 ,  Y10T436/10 ,  Y10T436/101666 ,  Y10T436/107497 ,  Y10T436/108331

摘要： A lytic reagent composition is provided which selectively stromatolyses red blood cells in a blood sample. In addition, a lytic reagent system is provided which enable the differentiation of at least two subpopulations of leukocytes. A method for using the lytic reagent system is also provided. Still further, the lytic reagent system find use in the determination of the hemoglobin in the blood. The lytic reagent system utilizes an ethoxylated long chain compound based lysis reagent with an acid and a solubilizer, and a hypertonic, alkaline, stabilizing reagent. The system and analysis method maintains the cellular morphology of the leukocytes and can be used to analyze normal and abnormal blood samples, fresh and aged blood, human and non-human animal blood samples, as well as other fluid samples such as bone marrow.

摘要翻译： 提供溶解试剂组合物，其选择性地在血液样品中进行红细胞分解。 此外，提供了使得能够分化白细胞的至少两个亚群的溶解试剂系统。 还提供了使用溶解试剂系统的方法。 此外，溶解试剂系统可用于测定血液中的血红蛋白。 裂解试剂系统使用具有酸和增溶剂的乙氧基化长链化合物裂解试剂和高渗，碱性稳定剂。 系统和分析方法维持白细胞的细胞形态，可用于分析正常和异常血液样本，新鲜和老化血液，人和非人动物血液样品以及其他流体样品如骨髓。