公开(公告)号：US5843608A

公开(公告)日：1998-12-01

申请号：US963784

申请日：1997-11-04

申请人： Yi Li ,  Carole Young ,  Timothy J. Fischer ,  James H. Carter ,  Sergio C. Veulens

发明人： Yi Li ,  Carole Young ,  Timothy J. Fischer ,  James H. Carter ,  Sergio C. Veulens

IPC分类号： G01N33/48 ,  G01N33/49 ,  G01N33/50 ,  G01N33/569 ,  G01N33/72 ,  G01N31/00

CPC分类号： G01N33/56972 ,  G01N33/5094 ,  G01N33/721 ,  Y10T436/10 ,  Y10T436/101666 ,  Y10T436/107497 ,  Y10T436/108331

摘要： A lytic reagent composition is provided which selectively stromatolyses red blood cells in a blood sample. In addition, a lytic reagent system is provided which enable the differentiation of at least two subpopulations of leukocytes. A method for using the lytic reagent system is also provided. Still further, the lytic reagent system find use in the determination of the hemoglobin in the blood. The lytic reagent system utilizes an ethoxylated long chain compound based lysis reagent with an acid and a solubilizer, and a hypertonic, alkaline, stabilizing reagent. The system and analysis method maintains the cellular morphology of the leukocytes and can be used to analyze normal and abnormal blood samples, fresh and aged blood, human and non-human animal blood samples, as well as other fluid samples such as bone marrow.

摘要翻译： 提供溶解试剂组合物，其选择性地在血液样品中进行红细胞分解。 此外，提供了使得能够分化白细胞的至少两个亚群的溶解试剂系统。 还提供了使用溶解试剂系统的方法。 此外，溶解试剂系统可用于测定血液中的血红蛋白。 裂解试剂系统使用具有酸和增溶剂的乙氧基化长链化合物裂解试剂和高渗，碱性稳定剂。 系统和分析方法维持白细胞的细胞形态，可用于分析正常和异常血液样本，新鲜和老化血液，人和非人动物血液样品以及其他流体样品如骨髓。

公开(公告)号：US5686308A

公开(公告)日：1997-11-11

申请号：US488630

申请日：1995-06-08

申请人： Yi Li ,  Carole Young ,  Timothy J. Fischer ,  James H. Carter

发明人： Yi Li ,  Carole Young ,  Timothy J. Fischer ,  James H. Carter

IPC分类号： G01N33/48 ,  G01N33/49 ,  G01N33/50 ,  G01N33/569 ,  G01N33/72 ,  G01N31/00

CPC分类号： G01N33/5094 ,  G01N33/56972 ,  G01N33/721 ,  Y10T436/10 ,  Y10T436/101666 ,  Y10T436/107497 ,  Y10T436/108331

摘要： A lytic reagent composition is provided which selectively stromatolyses red blood cells in a blood sample. In addition, a lytic reagent system is provided which enable the differentiation of at least two subpopulations of leukocytes. A method for using the lytic reagent system is also provided. Still further, the lytic reagent system find use in the determination of the hemoglobin in the blood. The lytic reagent system utilizes an ethoxylated long chain compound based lysis reagent with an acid and a hypertonic, alkaline, stabilizing reagent. The system and analysis method maintains the cellular morphology of the leukocytes and can be used to analyze normal and abnormal blood samples, fresh and aged blood, human and non-human animal blood samples, as well as other fluid samples such as bone marrow.

摘要翻译： 提供溶解试剂组合物，其选择性地在血液样品中进行红细胞分解。 此外，提供了使得能够分化白细胞的至少两个亚群的溶解试剂系统。 还提供了使用溶解试剂系统的方法。 此外，溶解试剂系统可用于测定血液中的血红蛋白。 裂解试剂系统利用具有酸和高渗，碱性稳定剂的乙氧基化长链化合物裂解试剂。 系统和分析方法维持白细胞的细胞形态，可用于分析正常和异常血液样本，新鲜和老化血液，人和非人动物血液样品以及其他流体样品如骨髓。

公开(公告)号：US5786224A

公开(公告)日：1998-07-28

申请号：US496469

申请日：1995-06-29

申请人： Yi Li ,  Carole Young ,  Timothy J. Fischer

发明人： Yi Li ,  Carole Young ,  Timothy J. Fischer

IPC分类号： G01N33/48 ,  G01N33/49 ,  G01N33/50

CPC分类号： G01N33/5094 ,  Y10T436/10 ,  Y10T436/101666 ,  Y10T436/107497 ,  Y10T436/25125 ,  Y10T436/2525

摘要： A lytic reagent composition is provided which selectively stromatolyses red blood cells in a blood sample. In addition, a lytic reagent system is provided which enables the differentiation of at least three subpopulations of leukocytes. A method for using the lytic reagent system is also provided. Still further, the lytic reagent system finds use in the determination of the hemoglobin in the blood. The lytic reagent system utilizes an alkyl sulfate, polyoxyethylene based surfactant and acid with a hypertonic, alkaline, stabilizing reagent. The system and analysis method maintains the cellular morphology of the leukocytes and can be used to analyze normal and abnormal blood samples, fresh and aged blood, human and non-human animal blood samples.

摘要翻译： 提供溶解试剂组合物，其选择性地在血液样品中进行红细胞分解。 此外，提供了一种裂解试剂系统，其能够分化至少三个亚群的白细胞。 还提供了使用溶解试剂系统的方法。 此外，溶解试剂系统用于测定血液中的血红蛋白。 溶解试剂系统使用烷基硫酸盐，聚氧乙烯基表面活性剂和酸与高渗，碱性稳定剂。 系统和分析方法维持白细胞的细胞形态，可用于分析正常和异常的血液样本，新鲜和老化的血液，人和非人动物血液样品。

公开(公告)号：US06362003B1

公开(公告)日：2002-03-26

申请号：US09152262

申请日：1998-09-14

申请人： Carole Young ,  Michael Elliott ,  Nancy Naylor ,  Timothy J. Fischer

发明人： Carole Young ,  Michael Elliott ,  Nancy Naylor ,  Timothy J. Fischer

IPC分类号： G01N3100

CPC分类号： G01N33/96 ,  G01N15/1012 ,  G01N2015/1037 ,  G01N2333/76 ,  G01N2496/05 ,  G01N2496/10 ,  G01N2496/25 ,  Y10T436/101666 ,  Y10T436/104165 ,  Y10T436/105831 ,  Y10T436/106664 ,  Y10T436/107497 ,  Y10T436/108331

摘要： A method of quality control to diagnose the cause of a malfunction of an instrument. The method uses measurements of the physical property of a sample to diagnose the cause of a malfunction of an instrument. The spatial position of a control product sample is analyzed. Alternatively, the spatial position of a statistically significant number of patient blood samples can be used. The method enables the monitoring of an instrument for problems associated with debris and noise caused by red cell lysis inefficiency; instrument reagents pump volume settings; instrument laser alignments; instrument gain settings; and flow noise caused by partial plugs, residual plugs or other flow problems. The method provides a more specific indication of the type and cause of an instrument malfunctioning than non specific flagging provided by prior art methods.

摘要翻译： 一种用于诊断仪器故障原因的质量控制方法。 该方法使用样品的物理性质的测量来诊断仪器故障的原因。 分析对照产品样品的空间位置。 或者，可以使用统计学上显着数量的患者血液样本的空间位置。 该方法能够监测仪器与由红细胞裂解无效率引起的碎片和噪声相关的问题; 仪器试剂泵体积设置; 仪器激光对准 仪器增益设置; 和由部分插头，残留插头或其他流量问题引起的流动噪声。 该方法提供了与现有技术方法提供的非特定标记相比，仪器故障的类型和原因的更具体的指示。

公开(公告)号：US5529933A

公开(公告)日：1996-06-25

申请号：US386711

申请日：1995-02-08

申请人： Carole Young ,  Michael N. Elliott ,  Nancy R. Naylor ,  Timothy J. Fischer

发明人： Carole Young ,  Michael N. Elliott ,  Nancy R. Naylor ,  Timothy J. Fischer

IPC分类号： G01N33/49 ,  G01N33/96

CPC分类号： G01N33/96 ,  G01N2496/05 ,  Y10T436/101666

摘要： A hematology control product comprising leukocyte analogs and an aqueous solution of a plasma substance for use in a blood counting instrument is described. The instrument employs a lytic reagent system for the lysable red blood cells in the control product. The plasma substance is in an amount effective to enable the differentiation of each of said leukocyte analogs relative to the physical attributes of the analogs, said physical attributes of the analog are similar to human leukocytes. Preferably, the plasma substance comprises cholesterol or its derivatives.A method for using the cell suspension media comprising an aqueous solution of a plasma substance is also described. The method provides a quality control to determine whether an instrument is operating within manufacturer's specifications.

摘要翻译： 描述了包含白细胞类似物和用于血液计数仪器的血浆物质的水溶液的血液控制产品。 该仪器在对照产品中使用溶解性红细胞的溶解试剂系统。 等离子体物质的量相对于类似物的物理属性有效地使每种所述白细胞类似物分化，所述类似物的所述物理属性类似于人白细胞。 优选地，血浆物质包含胆固醇或其衍生物。 还描述了使用包含等离子体物质的水溶液的细胞悬浮介质的方法。 该方法提供质量控制以确定仪器是否在制造商的规格范围内运行。

公开(公告)号：US5512485A

公开(公告)日：1996-04-30

申请号：US342997

申请日：1994-11-21

申请人： Carole Young ,  Michael N. Elliott ,  Timothy J. Fischer ,  Nancy R. Naylor

发明人： Carole Young ,  Michael N. Elliott ,  Timothy J. Fischer ,  Nancy R. Naylor

IPC分类号： G01N33/49 ,  G01N33/50 ,  G01N33/96 ,  G01N19/00

CPC分类号： G01N33/96 ,  G01N2496/05 ,  Y10T436/101666 ,  Y10T436/102499 ,  Y10T436/105831 ,  Y10T436/106664 ,  Y10T436/107497 ,  Y10T436/108331

摘要： A hematology control product comprising leukocyte analogs is described. The analogs comprise red blood cells which simulate at least two physical properties of human leukocytes. A method for making leukocyte analogs from blood cells having desired physical properties is also described. The process comprises expanding the cell volume, changing the hemoglobin content of the cell and fixing the cell. Generally, the monocyte and lymphocyte analogs leak hemoglobin from the cell while the eosinophil analog has the hemoglobin precipitated in the cell. A further method is described to use the control product to determine whether an automatic instrument is operating within manufacturer's specification.

摘要翻译： 描述了包含白细胞类似物的血液控制产品。 类似物包含模拟人白细胞的至少两种物理性质的红细胞。 还描述了从具有所需物理性质的血细胞制备白细胞类似物的方法。 该方法包括扩大细胞体积，改变细胞的血红蛋白含量和固定细胞。 通常，单核细胞和淋巴细胞类似物从细胞中泄漏血红蛋白，而嗜酸性粒细胞类似物在细胞中沉淀出血红蛋白。 描述了另一种方法来使用控制产品来确定自动仪器是否在制造商的规范内运行。

公开(公告)号：US5320964A

公开(公告)日：1994-06-14

申请号：US081752

申请日：1993-06-23

申请人： Carole Young ,  Michael N. Elliott ,  Timothy J. Fischer ,  Nancy R. Naylor

发明人： Carole Young ,  Michael N. Elliott ,  Timothy J. Fischer ,  Nancy R. Naylor

IPC分类号： G01N33/49 ,  G01N33/50 ,  G01N33/96 ,  G01N31/00

CPC分类号： G01N33/96 ,  G01N2496/05 ,  Y10T436/101666 ,  Y10T436/102499 ,  Y10T436/105831 ,  Y10T436/106664 ,  Y10T436/107497 ,  Y10T436/108331

摘要： A hematology control product comprising leukocyte analogs is described. The analogs comprise red blood cells which simulate at least two physical properties of human leukocytes. A method for making leukocyte analogs from blood cells having desired physical properties is also described. The process comprises expanding the cell volume, changing the hemoglobin content of the cell and fixing the cell. Generally, the monocyte and lymphocyte analogs leak hemoglobin from the cell while the eosinophil analog has the hemoglobin precipitated in the cell. A further method is described to use the control product to determine whether an automatic instrument is operating within manufacturer's specification.

摘要翻译： 描述了包含白细胞类似物的血液控制产品。 类似物包含模拟人白细胞的至少两种物理性质的红细胞。 还描述了从具有所需物理性质的血细胞制备白细胞类似物的方法。 该方法包括扩大细胞体积，改变细胞的血红蛋白含量和固定细胞。 通常，单核细胞和淋巴细胞类似物从细胞中泄漏血红蛋白，而嗜酸性粒细胞类似物在细胞中沉淀出血红蛋白。 描述了另一种方法来使用控制产品来确定自动仪器是否在制造商的规范内运行。

公开(公告)号：US20050074894A1

公开(公告)日：2005-04-07

申请号：US10955104

申请日：2004-09-30

申请人： Yi Li ,  Nery Ortiz ,  Carole Young ,  Santiago Galvez

发明人： Yi Li ,  Nery Ortiz ,  Carole Young ,  Santiago Galvez

IPC分类号： G01N21/00 ,  G01N31/00 ,  G01N33/48 ,  G01N33/569 ,  G01N33/80

CPC分类号： G01N15/1459 ,  G01N33/56972 ,  G01N33/80 ,  G01N33/96 ,  G01N2015/0073 ,  G01N2015/1062 ,  G01N2015/1486 ,  G01N2015/1493 ,  G01N2496/10 ,  Y10T436/10 ,  Y10T436/101666

摘要： Reference control compositions and the method of use are disclosed for measurement of nucleated red blood cells, which includes one set of synthetic spherical particles having a mean particle diameter ranging from 6.2 μm to 6.8 μm and a refractive index from 1.58 to 1.62 monodispersed in an aqueous suspension medium. The synthetic spherical particles have optical properties simulating optical properties of nucleated red blood cells as measured by optical measurements. The reference control composition can further include a second set of the synthetic spherical particles having optical properties simulating optical properties of white blood cells. Further disclosed is a reference control system which includes a series of reference control compositions, each having an amount of one type of synthetic spherical particles which have optical properties simulating the optical properties of nucleated red blood cells having a specific cell maturity.

公开(公告)号：US5882933A

公开(公告)日：1999-03-16

申请号：US898000

申请日：1997-07-25

申请人： Yi Li ,  Carole Young ,  Robert H. Raynor

发明人： Yi Li ,  Carole Young ,  Robert H. Raynor

IPC分类号： G01N33/48 ,  G01N15/14 ,  G01N33/49 ,  G01N33/50 ,  G01N33/569 ,  G01N33/72

CPC分类号： G01N33/721 ,  G01N15/12 ,  G01N15/1459 ,  G01N33/5094 ,  G01N33/56972 ,  G01N2015/1006 ,  G01N2015/1402 ,  G01N2015/1477 ,  Y10S436/824 ,  Y10T436/101666 ,  Y10T436/107497 ,  Y10T436/25 ,  Y10T436/25125 ,  Y10T436/2525

摘要： A method is provided for differentiating leukocyte subpopulations, immunophenotyping of lymphocytes and counting white blood cells. The method preserves leukocyte morphology and surface markers without using fixatives. In addition, the method finds utility in determination of hemoglobin concentration without using cyanide. The stable hemoglobin chromogen formed is measured at approximately 540 nm.

摘要翻译： 提供了一种分化白细胞亚群，淋巴细胞免疫表型和计数白细胞的方法。 该方法在不使用固定剂的情况下保留白细胞形态和表面标记。 此外，该方法在不使用氰化物的情况下可用于测定血红蛋白浓度。 在约540nm处测量形成的稳定的血红蛋白色原体。

公开(公告)号：US5763280A

公开(公告)日：1998-06-09

申请号：US786505

申请日：1997-01-21

申请人： Yi Li ,  Carole Young

发明人： Yi Li ,  Carole Young

IPC分类号： G01N33/49 ,  G01N33/50 ,  G01N33/569 ,  G01N33/72

CPC分类号： G01N33/721 ,  G01N33/5094 ,  G01N33/56972 ,  Y10T436/10 ,  Y10T436/101666 ,  Y10T436/105831 ,  Y10T436/107497 ,  Y10T436/108331 ,  Y10T436/25125

摘要： A cyanide-free lytic reagent composition and method for measuring the total hemoglobin concentration in a blood sample, for counting the number of leukocytes and for deferential counting of three leukocyte subpopulations including lymphocytes, monocytes, and granulocytes are described. The cyanide-free lytic reagent composition includes a hemolytic surfactant chosen from quaternary ammonium salts, pyridinium salts, organic phosphate esters, and alkyl sulfonates to lyse erythrocytes and release hemoglobin, and an organic ligand chosen from triazole and its derivatives, tetrazole and its derivatives, alkaline metal salts of oxonic acid, melamine, aniline-2-sulfonic acid, quinaldic acid, 2-amino-1,3,4-thiadiazole, triazine and its derivatives, urazole, DL-pipecolinic acid. isonicotinamide, anthranilonitrile, 6-aza-2-thiothymine, adenine, 3-(2-thienyl)acrylic acid, benzoic acid and alkali metal and ammonium salts of benzoic acid, and pyrazine and its derivatives to form a stable chromogen with hemoglobin. The lytic reagent composition has a pH ranging from about 1 to about 13. The lytic reagent composition is mixed with a blood sample which is prediluted with a suitable blood diluent and the UV absorption of the sample mixture is measured at the predetermined absorption wavelength of the formed chromogen from 510 nm to 560 nm. Counting the numbers of leukocytes and differential counting of three leukocyte subpopulations are accomplished simultaneously on an automated cell counter utilizing DC impedance measurement. Alternatively, the organic ligands can be added to a suitable blood diluent for hemoglobin and leukocyte analysis.

摘要翻译： 描述了一种无氰溶解试剂组合物和用于测量血液样品中总血红蛋白浓度，用于计数白细胞数和用于对包括淋巴细胞，单核细胞和粒细胞在内的三种白细胞亚群进行精确计数的方法。 无氰溶解试剂组合物包括选自季铵盐，吡啶鎓盐，有机磷酸酯和烷基磺酸盐以溶解红细胞并释放血红蛋白的溶血性表面活性剂，以及选自三唑及其衍生物，四唑及其衍生物的有机配体， 硝基酸，三聚氰胺，苯胺-2-磺酸，喹喔啉酸，2-氨基-1,3,4-噻二唑，三嗪及其衍生物的碱金属盐，脲，DL-哌啶酸。 异烟酰胺，邻氨基苯甲腈，6-氮杂-2-硫代胸腺嘧啶，腺嘌呤，3-（2-噻吩基）丙烯酸，苯甲酸和苯甲酸的碱金属和铵盐，吡嗪及其衍生物，与血红蛋白形成稳定的色原体。 溶解试剂组合物的pH为约1至约13.溶液试剂组合物与用合适的血液稀释剂预稀释的血液样品混合，并且在预定的吸收波长下测量样品混合物的UV吸收 形成了510nm至560nm的色原子。 使用直流阻抗测量，在自动细胞计数器上同时实现计数白细胞数量和三个白细胞亚群的差异计数。 或者，可以将有机配体加入合适的血液稀释液中用于血红蛋白和白细胞分析。