公开(公告)号：US20180237752A1

公开(公告)日：2018-08-23

申请号：US15958824

申请日：2018-04-20

Applicant: Janssen Biotech, Inc.

Inventor： Benjamin Fryer

IPC: C12N5/071

CPC classification number: C12N5/0678 ,  C12N2500/34 ,  C12N2500/90 ,  C12N2501/115 ,  C12N2501/19 ,  C12N2501/727 ,  C12N2506/02 ,  C12N2533/54

Abstract: The present invention provides methods to promote the differentiation of pluripotent stem cells into insulin producing cells. In particular, the present invention provides a method to produce a population of cells, wherein greater than 80% of the cells in the population express markers characteristic of the definitive endoderm lineage.

公开(公告)号：US20170009212A1

公开(公告)日：2017-01-12

申请号：US15274749

申请日：2016-09-23

Applicant: Janssen Biotech, Inc.

Inventor： Benjamin Fryer

IPC: C12N5/071

CPC classification number: C12N5/0678 ,  C12N2500/34 ,  C12N2500/90 ,  C12N2501/115 ,  C12N2501/19 ,  C12N2501/727 ,  C12N2506/02 ,  C12N2533/54

Abstract: The present invention provides methods to promote the differentiation of pluripotent stem cells into insulin producing cells. In particular, the present invention provides a method to produce a population of cells, wherein greater than 80% of the cells in the population express markers characteristic of the definitive endoderm lineage.

Abstract translation: 本发明提供促进多能干细胞分化成胰岛素生成细胞的方法。 特别地，本发明提供了一种产生细胞群的方法，其中群体中大于80％的细胞表达定形内胚层谱系特征的标志物。

公开(公告)号：US20160215268A1

公开(公告)日：2016-07-28

申请号：US14963730

申请日：2015-12-09

Applicant: Janssen Biotech, Inc.

Inventor： Benjamin Fryer ,  Daina Laniauskas

IPC: C12N5/071

CPC classification number: C12N5/0676 ,  C12N2500/02 ,  C12N2500/25 ,  C12N2500/38 ,  C12N2500/60 ,  C12N2501/117 ,  C12N2501/155 ,  C12N2501/16 ,  C12N2501/19 ,  C12N2501/385 ,  C12N2501/395 ,  C12N2501/41 ,  C12N2501/415 ,  C12N2501/727 ,  C12N2501/91 ,  C12N2501/999 ,  C12N2506/02

Abstract: The present invention provides methods of differentiating pluripotent cells into beta cell using suspension clustering. The methods of the invention use control of one or more of pH, cell concentration, and retinoid concentration to generate a nearly homogenous population of PDX1/NKX6.1 co-expressing cells by suppressing precocious NGN3 expression and promoting NKX6.1 expression. Also, the nearly homogenous population of PDX1/NKX6.1 co-expressing cells may be further differentiated in vitro to form a population of pancreatic endocrine cells that co-express PDX1, NKX6.1, insulin and MAFA.

Abstract translation: 本发明提供了使用悬浮聚类将多能细胞分化成β细胞的方法。 本发明的方法是通过抑制早期的NGN3表达和促进NKX6.1表达来控制pH，细胞浓度和类视色素浓度中的一种或多种，​​以产生几乎均匀的PDX1 / NKX6.1共表达细胞群。 此外，PDX1 / NKX6.1共表达细胞的几乎均匀的群体可以在体外进一步分化以形成共表达PDX1，NKX6.1，胰岛素和MAFA的胰腺内分泌细胞群体。

公开(公告)号：US20140242693A1

公开(公告)日：2014-08-28

申请号：US13998974

申请日：2013-12-30

Applicant: Janssen Biotech, Inc.

Inventor： Benjamin Fryer ,  Daina Laniauskas ,  Marcia Blackmoore ,  Haiyun Wang ,  Kostadinka LiLova ,  Shelly Nelson ,  Elizabeth Rosocha

IPC: C12N5/071

CPC classification number: C12N5/0677 ,  C12N5/0606 ,  C12N2500/02 ,  C12N2500/25 ,  C12N2500/34 ,  C12N2501/117 ,  C12N2501/15 ,  C12N2501/16 ,  C12N2501/19 ,  C12N2501/385 ,  C12N2501/415 ,  C12N2501/42 ,  C12N2501/727 ,  C12N2501/999 ,  C12N2506/02 ,  C12N2506/45 ,  C12N2509/00 ,  C12N2513/00 ,  C12N2531/00

Abstract: The present invention provides methods of preparing aggregated pluripotent stem cell clusters for differentiation.

Abstract translation: 本发明提供了用于分化的聚集多能干细胞簇的方法。

公开(公告)号：US10370644B2

公开(公告)日：2019-08-06

申请号：US14307100

申请日：2014-06-17

Applicant: Janssen Biotech, Inc.

Inventor： Benjamin Fryer ,  Daina Laniauskas ,  Marcia Blackmoore ,  Haiyun Wang ,  Kostadinka Lilova ,  Shelley Nelson ,  Elizabeth Rosocha

IPC: C12N5/074 ,  C12N5/071

Abstract: The present invention provides methods of preparing aggregated pluripotent stem cell clusters for differentiation. Specifically, the invention discloses methods of differentiating pluripotent cells into beta cell, cardiac cell and neuronal cell lineages using suspension clustering. The methods involve preparing the aggregated cell clusters followed by differentiation of these clusters.