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11.发明申请Expression of zeta negative and zeta positive nucleic acids using a dystrophin gene 审中-公开使用肌营养不良蛋白基因表达ζ阴性和ζ阳性核酸公开(公告)号：US20070173465A9
公开(公告)日：2007-07-26
申请号：US10339934
申请日：2003-01-10
申请人： Sean Monahan , Jon Wolff , Paul Slattum , James Hagstrom , Vladimir Budker , David Rozema
发明人： Sean Monahan , Jon Wolff , Paul Slattum , James Hagstrom , Vladimir Budker , David Rozema
CPC分类号： A61K48/0016 , A61K48/0041 , A61K48/0075 , A61K48/0083
摘要： Disclosed is expression of zeta negative and zeta positive nucleic acids or nucleic acid complexes using a dystrophin gene in a process for providing nucleic acid expression in a striated (skeletal or cardiac) muscle cell for the purpose of providing a change to the endogenous properties of the cell for cells affected by muscular dystrophy.
摘要翻译： 公开了在用于在条纹（骨骼或心脏）肌细胞中提供核酸表达的过程中使用肌营养不良蛋白基因的ζ阴性和ζ阳性核酸或核酸复合物的表达，以提供对 细胞受肌营养不良影响的细胞。
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公开(公告)号：US20070010004A1
公开(公告)日：2007-01-11
申请号：US11479587
申请日：2006-06-30
申请人： Sean Monahan , Vladimir Budker , Tatyana Budker , Jon Wolff , Paul Slattum , James Hagstrom
发明人： Sean Monahan , Vladimir Budker , Tatyana Budker , Jon Wolff , Paul Slattum , James Hagstrom
CPC分类号： C07H21/00
摘要： Methods are described for modifying nucleic acids to facilitate delivery of the nucleic acids to cells. Compounds which interact with of modify nucleic acids are interacted with the nucleic acids within reverse micelles.
摘要翻译： 描述了用于修饰核酸以便于将核酸递送至细胞的方法。 与修饰核酸相互作用的化合物与反胶束内的核酸相互作用。
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公开(公告)号：US20050272154A1
公开(公告)日：2005-12-08
申请号：US11189321
申请日：2005-07-26
申请人： Jon Wolff , Vladimir Budker , James Hagstrom , Paul Slattum
发明人： Jon Wolff , Vladimir Budker , James Hagstrom , Paul Slattum
摘要： A process and compound wherein nucleic acids can be modified with a host of molecules and maintain their ability to be expressed. A modifying chemical attachment of polyions to polynucleotides can be used to facilitate the change of tertiary structure of the nucleic acid and in some cases condensation of nucleic acids into smaller, charged particles useful in delivering the nucleic acid to a cell.
摘要翻译： 一种方法和化合物，其中核酸可以用多种分子修饰并保持其表达能力。 可以将聚阴离子与多核苷酸的修饰化学连接用于促进核酸的三级结构的改变，并且在一些情况下，将核酸缩合成用于将核酸递送至细胞的较小带电粒子。
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14.发明申请公开(公告)号：US20060188927A1
公开(公告)日：2006-08-24
申请号：US11415021
申请日：2006-05-01
申请人： Paul Slattum , Jon Wolff , James Hagstrom , Vladimir Budker
发明人： Paul Slattum , Jon Wolff , James Hagstrom , Vladimir Budker
CPC分类号： C07D209/08 , C07D209/14 , C07H19/00 , C07H21/04
摘要： Compounds and methods are provided for a single-pot covalent attachment of a label to nucleic acids comprising forming a covalently attachable labeling reagent for alkylating the molecule. Then, combining the covalently attachable labeling reagent with a mixture containing the molecule, under conditions wherein the labeling reagent has reactivity with the molecule thereby forming a covalent bond.
摘要翻译： 提供了化合物和方法用于标记物与核酸的单罐共价连接，包括形成用于烷基化分子的共价连接的标记试剂。 然后，在标记试剂与分子具有反应性，从而形成共价键的条件下，将可共价连接的标记试剂与含有该分子的混合物结合。
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公开(公告)号：US20060159764A1
公开(公告)日：2006-07-20
申请号：US11322419
申请日：2005-12-30
申请人： Sean Monahan , David Rozema , Vladimir Trubetskoy , Paul Slattum , Jon Wolff , Vladimir Budker , James Hagstrom , Lisa Hanson
发明人： Sean Monahan , David Rozema , Vladimir Trubetskoy , Paul Slattum , Jon Wolff , Vladimir Budker , James Hagstrom , Lisa Hanson
CPC分类号： C12N15/87 , A61K47/59 , A61K47/60 , A61K47/645 , A61K48/00 , A61K48/0008 , A61K48/0025 , A61K48/0041
摘要： A method of forming polymers in the presence of nucleic acid using template polymerization. These methods can be used for the delivery of nucleic acids, for condensing the nucleic acid, for forming nucleic acid binding polymers, for forming supramolecular complexes containing nucleic acid and polymer, and for forming an interpolyelectrolyte complex.
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公开(公告)号：US20060063257A1
公开(公告)日：2006-03-23
申请号：US11256322
申请日：2005-10-21
申请人： Darren Wakefield , David Rozema , Jon Wolff , Vladimir Trubetskoy , James Hagstrom , Vladimir Budker , Jason Klein , So Wong
发明人： Darren Wakefield , David Rozema , Jon Wolff , Vladimir Trubetskoy , James Hagstrom , Vladimir Budker , Jason Klein , So Wong
IPC分类号： C12N15/87
CPC分类号： C12N15/87 , A61K47/6455 , C12N15/88
摘要： Polyampholyte are able to condense nucleic acid to form small complexes which can be utilized in the delivery of nucleic acid to mammalian cells. The polyampholytes can be formed prior to interaction with nucleic acid or they can be formed in the presence of nucleic acid. Stabilized polycation/nucleic acid complexes can be modified to reduce the positive charge of the polycation and add targeting ligands without destabilizing the complex. The resultant particles retain their small size and are more effective in delivery of nucleic acid to cells in vivo.
摘要翻译： 聚阴离子能够冷凝核酸以形成可用于将核酸递送至哺乳动物细胞的小复合物。 聚阴离子可以在与核酸相互作用之前形成，或者可以在核酸存在下形成。 可以修饰稳定的聚阳离子/核酸复合物以减少聚阳离子的正电荷并添加靶向配体而不使复合物失稳。 所得颗粒保留其小尺寸并且在体内将核酸递送至细胞更有效。
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公开(公告)号：US20060024828A1
公开(公告)日：2006-02-02
申请号：US11235000
申请日：2005-09-26
申请人： Vladimir Trubetskoy , Jon Wolff , Paul Slattum , Lisa Hanson , Vladimir Budker , James Hagstrom
发明人： Vladimir Trubetskoy , Jon Wolff , Paul Slattum , Lisa Hanson , Vladimir Budker , James Hagstrom
CPC分类号： C12N15/87 , A61K47/59 , A61K47/60 , A61K47/645 , A61K48/00 , A61K48/0008 , A61K48/0025 , A61K48/0041
摘要： A method of forming polymers in the presence of nucleic acid using template polymerization. Also, a method of having the polymerization occur in heterophase systems. These methods can be used for the delivery of nucleic acids, for condensing the nucleic acid, for forming nucleic acid binding polymers, for forming supramolecular complexes containing nucleic acid and polymer, and for forming an interpolyelectrolyte complex.
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公开(公告)号：US20050123600A1
公开(公告)日：2005-06-09
申请号：US11046590
申请日：2005-01-28
申请人： Vladimir Trubetskoy , James Hagstrom , Vladimir Budker , Jon Wolff , David Rozema , Sean Monahan
发明人： Vladimir Trubetskoy , James Hagstrom , Vladimir Budker , Jon Wolff , David Rozema , Sean Monahan
CPC分类号： C12N15/88 , A61K47/60 , A61K47/645
摘要： An polyampholyte is utilized in a condensed polynucleotide complex for purposes of nucleic acid delivery to a cell. The complex can be formed with an appropriate amount of positive and/or negative charge such that the resulting complex can be delivered to the extravascular space and may be further delivered to a cell.
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19.发明申请Compositions and processes using siRNA, amphipathic compounds and polycations 有权使用siRNA，两亲性化合物和聚阳离子的组合物和方法公开(公告)号：US20050143332A1
公开(公告)日：2005-06-30
申请号：US10845968
申请日：2004-05-14
申请人： Sean Monahan , David Lewis , Hans Herweijer , Jon Wolff , James Hagstrom , Aaron Loomis , Vladimir Trubetskoy , Lori Higgs
发明人： Sean Monahan , David Lewis , Hans Herweijer , Jon Wolff , James Hagstrom , Aaron Loomis , Vladimir Trubetskoy , Lori Higgs
IPC分类号： A61K31/495 , A61K31/58 , A61K31/7105 , A61K31/74 , A61K48/00 , C07H21/04 , C12N15/11 , C12N15/88 , G01N33/53
CPC分类号： C12N15/111 , A61K31/495 , A61K31/58 , A61K31/7105 , A61K31/74 , A61K48/0025 , A61K48/0041 , C07H21/04 , C12N15/88 , C12N2310/14 , C12N2320/32 , A61K2300/00
摘要： Described is a deliverable composition with low toxicity comprising an amphipathic compound, a polycation, and a siRNA. The composition may be used in the process of delivering a siRNA to an animal cell or more particularly, a mammal cell.
摘要翻译： 描述了具有低毒性的可递送组合物，其包含两亲性化合物，聚阳离子和siRNA。 组合物可以用于将siRNA递送到动物细胞或更特别是哺乳动物细胞的过程中。
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20.发明申请Inhibition of gene function by delivery of polynucleotide-based gene expression inhibitors to mammalian cells in vivo 审中-公开通过在体内向哺乳动物细胞递送基于多核苷酸的基因表达抑制剂来抑制基因功能公开(公告)号：US20050037989A1
公开(公告)日：2005-02-17
申请号：US10874528
申请日：2004-06-23
申请人： David Lewis , Jon Wolff , Hans Herweijer , James Hagstrom , Aaron Loomis , Andrew Spencer
发明人： David Lewis , Jon Wolff , Hans Herweijer , James Hagstrom , Aaron Loomis , Andrew Spencer
IPC分类号： A61K38/00 , A61K48/00 , C12N15/11 , C12N15/113 , C12N15/87
CPC分类号： C12N15/111 , A61K38/00 , A61K48/00 , C12N15/1137 , C12N15/87 , C12N2310/14 , C12N2320/32 , C12Y206/01002
摘要： A process is provided to deliver polynucleotide-based gene expression inhibitors to cells in a mammal in vivo for the purpose of inhibiting gene expression in the cells. Inhibition is sequence-specific and relies on sequence similaroty of the polynucleotide-based gene expression inhibitor and the target nucleic acid molecule. Delivery of the polynucleotide-based gene expression inhibitor can enhance the efficacy of co-delivered small molecule drugs.
摘要翻译： 为了抑制细胞中的基因表达，提供了一种用于在体内向哺乳动物中的细胞递送基于多核苷酸的基因表达抑制剂的方法。 抑制是序列特异性的，并且依赖于基于多核苷酸的基因表达抑制剂和靶核酸分子的序列相似性。 基于多核苷酸的基因表达抑制剂的递送可以增强共同递送的小分子药物的功效。
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