公开(公告)号：US20220380755A1

公开(公告)日：2022-12-01

申请号：US17770803

申请日：2020-10-22

Applicant: JumpCode Genomics, Inc.

Inventor： Keith Brown

IPC: C12N15/10 ,  G16B30/20 ,  C12Q1/6869 ,  C12Q1/6855 ,  C12Q1/6806

Abstract: Provided are methods for preparation and analysis of nucleic acids. Some embodiments include reverse transcribing the RNA with barcoded primers to produce cDNA while maintaining the DNA in the sample, sequencing the DNA and cDNA together, and differentiating the sequenced DNA and cDNA using the barcode or barcodes of the primers. Some embodiments include analyzing the DNA and cDNA sequences of multiple samples separating reads into k-mers, and comparing the k-mers between samples to identify differential sequences between the sequences of the samples.

公开(公告)号：US20220259649A1

公开(公告)日：2022-08-18

申请号：US17724309

申请日：2022-04-19

Applicant: JumpCode Genomics, Inc.

Inventor： Keith Brown

IPC: C12Q1/6869 ,  C12Q1/6865

Abstract: Disclosed herein are methods of long range target specific amplification and sequencing using an RNA intermediate synthesized directly from the target including using hairpin adaptors having a double stranded promoter and an overhang which hybridizes with a reverse-complementary overhang on a target nucleic acid. RNA transcription eliminates clonal amplification of early synthesis errors. Approaches allow for the identification of target-adjacent sequence, such as sequence adjacent to a repeat element target. Also disclosed herein are compositions and kits for amplification and sequencing.

公开(公告)号：US20200181700A1

公开(公告)日：2020-06-11

申请号：US16793908

申请日：2020-02-18

Applicant: JumpCode Genomics, Inc.

Inventor： Keith Brown ,  Peter Dansky

IPC: C12Q1/6874 ,  C12N15/10 ,  C12Q1/6811 ,  C12Q1/68

Abstract: Disclosed herein are compositions and methods related to the elimination of molecules of a selected sequence from a nucleic acid sample or from an sequence dataset resulting from the sequencing of a sample, for example to exclude such molecules from downstream analysis or sequencing, or to exclude such sequences from a downstream data set.

公开(公告)号：US20180237950A1

公开(公告)日：2018-08-23

申请号：US15553495

申请日：2016-02-25

Applicant: JumpCode Genomics, Inc. ,  The Scripps Research Institute

Inventor： Keith Brown ,  Daniel R. Salomon ,  Steven Head ,  Azeem Siddique ,  Phillip Ordoukhanian

IPC: C40B40/08

CPC classification number: C40B40/08 ,  C12N15/1093

Abstract: Methods, compositions and kits are provided herein for insertional modification of nucleic acids by, for example transposase-mediated covalent insertion of insertion sequence into a sample nucleic acid molecule. Using sequence of the insertion to direct amplification of adjacent nucleic acid sequence, and using bar codes to map amplified sequence to partitions, one can map sample nucleic acid sequence to single molecules of the nucleic acid sample that are derived directly from the sample nucleic acid molecule.