公开(公告)号：US20160237462A1

公开(公告)日：2016-08-18

申请号：US14437672

申请日：2013-10-22

Applicant: KANEKA CORPORATION

Inventor： Hisashi ARIKAWA ,  Shunsuke SATO ,  Keiji MATSUMOTO

IPC: C12P7/62 ,  C12N15/74

CPC classification number: C12P7/625 ,  C12N9/18 ,  C12N15/74

Abstract: An object of the present invention is to provide a microorganism strain that accumulates a high molecular weight PHA, and a PHA production method using the microorganism. The present invention provides a method for producing a PHA copolymer, which includes culturing a microorganism, wherein at least a portion of either of the following genes (a) and (b) of the microorganism has been altered by substitution, deletion, insertion, and/or addition to reduce or eliminate the activity of a PHA degrading enzyme encoded by the gene: (a) a PHA degrading enzyme gene encoding the amino acid sequence of SEQ ID NO:2 in the sequence listing; and (b) a gene encoding a polypeptide having at least 85% sequence identity to the amino acid sequence of SEQ ID NO:2 in the sequence listing and having PHA degrading enzyme activity.

Abstract translation: 本发明的目的是提供一种积累高分子量PHA的微生物菌株和使用该微生物的PHA生产方法。 本发明提供一种生产PHA共聚物的方法，其包括培养微生物，其中微生物的以下基因（a）和（b）中的至少一部分已经被替换，缺失，插入和 /或添加以减少或消除由该基因编码的PHA降解酶的活性：（a）编码序列表中SEQ ID NO：2的氨基酸序列的PHA降解酶基因; 和（b）编码与序列表中SEQ ID NO：2的氨基酸序列具有至少85％序列同一性且具有PHA降解酶活性的多肽的基因。