公开(公告)号：US20210388324A1

公开(公告)日：2021-12-16

申请号：US17412340

申请日：2021-08-26

Applicant: KANEKA CORPORATION

Inventor： Hisashi ARIKAWA ,  Shunsuke SATO

IPC: C12N9/10 ,  C12P7/62

Abstract: Provided is a transformed microorganism that has a polyhydroxyalkanoate synthase gene and in which expression of a minD gene is enhanced. Also provided is a transformed microorganism that has a polyhydroxyalkanoate synthase gene and in which expression of a minC gene and a minD gene is enhanced. In this transformed microorganism, expression of a minE gene may be enhanced or reduced. Also provided is a method of producing a PHA, the method including the step of culturing any of the transformed microorganisms in the presence of a carbon source.

公开(公告)号：US20220411830A1

公开(公告)日：2022-12-29

申请号：US17763886

申请日：2020-08-05

Applicant: KANEKA CORPORATION

Inventor： Hisashi ARIKAWA ,  Shunsuke SATO ,  Yoshihiro MOURI

IPC: C12P7/625

Abstract: A polyhydroxyalkanoate-producing microorganism is cultured to obtain microbial bodies accumulating polyhydroxyalkanoate particles and having an average cell size of 2 μm or more. The microbial bodies are subjected to a heat treatment at a temperature higher than a temperature in the culturing to increase an average particle size of the polyhydroxyalkanoate particles in the microbial bodies. The resulting average particle size is equal to or greater than 1.8 μm and equal to or smaller than the average cell size. The microbial bodies subjected to the heat treatment can be disrupted to obtain a cell disruption solution. The PHA particles can be separated from an aqueous phase of the cell disruption solution.

公开(公告)号：US20220315958A1

公开(公告)日：2022-10-06

申请号：US17641358

申请日：2020-08-04

Applicant: KANEKA CORPORATION

Inventor： Yoshihiro MOURI ,  Hisashi ARIKAWA ,  Shunsuke SATO

IPC: C12P7/625 ,  C12N1/20 ,  C12N15/74 ,  C12N9/10

Abstract: Provided is a transformed microorganism that has a polyhydroxyalkanoate synthase gene and in which expression of an A1386 gene and/or an A2405 gene is reduced. In the transformed microorganism, expression of a minC gene and a minD gene may be enhanced. Also provided is a method of producing a PHA, the method including the step of culturing the transformed microorganism in the presence of a carbon source.

公开(公告)号：US20170198313A1

公开(公告)日：2017-07-13

申请号：US15129726

申请日：2015-03-27

Applicant: KANEKA CORPORATION

Inventor： Shingo KOBAYASHI ,  Rina AOKI ,  Tetsuya MINAMI ,  Hisashi ARIKAWA ,  Keiji MATSUMOTO

IPC: C12P7/62 ,  C12N9/88 ,  C12N9/10

CPC classification number: C12P7/625 ,  C08G63/06 ,  C08L101/16 ,  C12N9/1025 ,  C12N9/1029 ,  C12N9/88 ,  C12N15/09 ,  C12Y203/01 ,  C12Y402/01017

Abstract: A PHA copolymer which is slowly crystallized is improved in crystallization speed to improve the melt workability of the PHA copolymer in working such as injection molding, film molding, blow molding, fiber spinning, extrusion foaming or bead foaming, thereby improving the resultant articles in productivity. A method for the improvement is a method for producing a PHA mixture, including the step of culturing a microorganism having both of a gene encoding a PHA synthase that synthesizes a copolymer PHA (A) and that is derived from the genus Aeromonas, and a gene encoding a PHA synthase that synthesizes a PHA (B) different in melting point from the copolymer PHA (A) by 10° C. or more to produce, in a cell of the microorganism, two or more PHAs different in melting point from one another by 10° C. or more simultaneously.

公开(公告)号：US20240417742A1

公开(公告)日：2024-12-19

申请号：US18695099

申请日：2022-09-28

Applicant: KANEKA CORPORATION

Inventor： Ken HARADA ,  Shunsuke SATO ,  Hisashi ARIKAWA

IPC: C12N15/74 ,  C12N1/20 ,  C12N9/02 ,  C12N9/10 ,  C12P7/625

Abstract: A microorganism is cultivated in the presence of a carbon source. The microorganism is a transformed microorganism having an introduced fadH gene encoding 2,4-dienoyl-CoA reductase. That is, the transformed microorganism has an introduced foreign gene encoding a protein having an amino acid sequence of SEQ ID NO: 1 or an introduced foreign gene encoding a protein having an amino acid sequence that is at least 85% identical to the amino acid sequence of SEQ ID NO: 1. The carbon source contains an oil containing unsaturated fatty acids as constituents or free unsaturated fatty acids.

公开(公告)号：US20180371509A1

公开(公告)日：2018-12-27

申请号：US16061923

申请日：2016-12-14

Applicant: KANEKA CORPORATION

Inventor： Hisashi ARIKAWA ,  Tetsuya FUJIKI

IPC: C12P7/62 ,  C08G63/06 ,  C12N9/10 ,  C12N15/52 ,  C12N1/20

Abstract: An object of the present invention is to provide a PHA-producing microorganism which can assimilate sucrose, and a method for producing a PHA by culturing this microorganism, using sucrose as a carbon source. A PHA-producing microorganism, comprising a PHA synthase gene, and heterogeneous-organism-derived genes in the following items (1) and (2): (1) a sucrose hydrolase gene encoding an amino acid sequence described in SEQ ID NO: 1, or a gene encoding a polypeptide which has a sequence homology of 90% or more to the amino acid sequence and which has sucrose hydrolase activity; and (2) a sucrose permease gene encoding an amino acid sequence described in SEQ ID NO: 2, or a gene encoding a polypeptide which has a sequence homology of 90% or more to the amino acid sequence and which has sucrose permease activity. The invention is also a method for producing a PHA, including the step of culturing this microorganism in a medium including sucrose as a carbon source.

公开(公告)号：US20240191266A1

公开(公告)日：2024-06-13

申请号：US17995827

申请日：2021-04-08

Applicant: KANEKA CORPORATION

Inventor： Hisashi ARIKAWA ,  Shunsuke SATO ,  Sho FURUTATE

IPC: C12P7/625 ,  C12N1/20 ,  C12N9/10 ,  C12N9/88 ,  C12N15/74

CPC classification number: C12P7/625 ,  C12N1/20 ,  C12N9/1029 ,  C12N9/88 ,  C12N15/74 ,  C12Y402/01017

Abstract: Provided is a method of producing a polyhydroxyalkanoate copolymer mixture. The method includes the step of culturing a microorganism that produces the polyhydroxyalkanoate copolymer mixture. The mixture contains: a fraction (I) containing a polyhydroxyalkanoate copolymer having a 3-hydroxybutyrate structural unit and a 3-hydroxyhexanoate structural unit, the fraction (I) having an average 3-hydroxyhexanoate unit ratio of 20 mol % or more; and a fraction (II) containing a polyhydroxyalkanoate having a 3-hydroxybutyrate structural unit, the fraction (II) having an average 3-hydroxyhexanoate unit ratio of 0 to 15 mol %. The polyhydroxyalkanoate copolymer mixture has an average 3-hydroxyhexanoate unit ratio of 22 mol % or less.

公开(公告)号：US20200340020A1

公开(公告)日：2020-10-29

申请号：US16927229

申请日：2020-07-13

Applicant: KANEKA CORPORATION

Inventor： Hisashi ARIKAWA ,  Shunsuke Sato

IPC: C12P7/62 ,  C12N9/10

Abstract: The present application provides: a transformed microorganism for producing a PHA copolymer containing 3HH monomer unit at a higher composition ratio, specifically, a transformed microorganism comprising a PHA synthase gene capable of synthesizing a PHA copolymer containing 3HH monomer unit and a gene encoding a protein having (R)-specific enoyl-CoA hydratase activity, characterized in that, in the transformed microorganism, the expression of a gene encoding at least one β-ketothiolase enzyme having thiolysis activity for β-keto-(C6) acyl-CoA (i.e., β-ketohexanoyl-CoA) is inhibited, thereby losing or reducing the enzyme activity; and a method for producing a PHA copolymer containing 3HH monomer unit, comprising a step of culturing the transformed microorganism.

公开(公告)号：US20200056214A1

公开(公告)日：2020-02-20

申请号：US16670042

申请日：2019-10-31

Applicant: KANEKA CORPORATION

Inventor： Shingo KOBAYASHI ,  Rina AOKI ,  Tetsuya MINAMI ,  Hisashi ARIKAWA ,  Keiji MATSUMOTO

IPC: C12P7/62 ,  C12N9/88 ,  C12N9/10 ,  C08G63/06

Abstract: A PHA copolymer which is slowly crystallized is improved in crystallization speed to improve the melt workability of the PHA copolymer in working such as injection molding, film molding, blow molding, fiber spinning, extrusion foaming or bead foaming, thereby improving the resultant articles in productivity. A method for the improvement is a method for producing a PHA mixture, including the step of culturing a microorganism having both of a gene encoding a PHA synthase that synthesizes a copolymer PHA (A) and that is derived from the genus Aeromonas, and a gene encoding a PHA synthase that synthesizes a PHA (B) different in melting point from the copolymer PHA (A) by 10° C. or more to produce, in a cell of the microorganism, two or more PHAs different in melting point from one another by 10° C. or more simultaneously.

公开(公告)号：US20170009221A1

公开(公告)日：2017-01-12

申请号：US15115394

申请日：2015-01-30

Applicant: KANEKA CORPORATION

Inventor： Hisashi ARIKAWA ,  Shunsuke SATO ,  Keiji MATSUMOTO

IPC: C12N9/88 ,  C12P7/62 ,  C12N15/74

CPC classification number: C12N9/88 ,  C12N15/74 ,  C12P7/625 ,  C12Y402/01017 ,  C12Y402/01074

Abstract: This invention relates to a microorganism that produces a polyhydroxyalkanoate (PHA) copolymer with a regulated monomer composition ratio and comprises a (R)-specific enoyl-CoA hydratase gene in the genome DNA, wherein a nucleotide sequence upstream of the (R)-specific enoyl-CoA hydratase gene comprises a modification consisting of a substitution(s), a deletion(s), an insertion(s), and/or an addition(s) of one or a plurality of nucleotides so that the expression of the (R)-specific enoyl-CoA hydratase gene is regulated, and to a method for producing a PHA copolymer using the microorganism.

Abstract translation: 本发明涉及产生具有调节的单体组成比的聚羟基链烷酸酯（PHA）共聚物的微生物，并且在基因组DNA中包含（R） - 特异性烯酰辅酶A水合酶基因，其中（R） - 特异性 烯酰辅酶A水合酶基因包含由一个或多个核苷酸的取代，缺失，插入和/或添加组成的修饰物，使得（ R） - 特异性烯酰辅酶A水合酶基因，以及使用该微生物生产PHA共聚物的方法。