公开(公告)号：US20220315958A1

公开(公告)日：2022-10-06

申请号：US17641358

申请日：2020-08-04

Applicant: KANEKA CORPORATION

Inventor： Yoshihiro MOURI ,  Hisashi ARIKAWA ,  Shunsuke SATO

IPC: C12P7/625 ,  C12N1/20 ,  C12N15/74 ,  C12N9/10

Abstract: Provided is a transformed microorganism that has a polyhydroxyalkanoate synthase gene and in which expression of an A1386 gene and/or an A2405 gene is reduced. In the transformed microorganism, expression of a minC gene and a minD gene may be enhanced. Also provided is a method of producing a PHA, the method including the step of culturing the transformed microorganism in the presence of a carbon source.

公开(公告)号：US20220411830A1

公开(公告)日：2022-12-29

申请号：US17763886

申请日：2020-08-05

Applicant: KANEKA CORPORATION

Inventor： Hisashi ARIKAWA ,  Shunsuke SATO ,  Yoshihiro MOURI

IPC: C12P7/625

Abstract: A polyhydroxyalkanoate-producing microorganism is cultured to obtain microbial bodies accumulating polyhydroxyalkanoate particles and having an average cell size of 2 μm or more. The microbial bodies are subjected to a heat treatment at a temperature higher than a temperature in the culturing to increase an average particle size of the polyhydroxyalkanoate particles in the microbial bodies. The resulting average particle size is equal to or greater than 1.8 μm and equal to or smaller than the average cell size. The microbial bodies subjected to the heat treatment can be disrupted to obtain a cell disruption solution. The PHA particles can be separated from an aqueous phase of the cell disruption solution.

公开(公告)号：US20220258387A1

公开(公告)日：2022-08-18

申请号：US17627428

申请日：2020-06-08

Applicant: KANEKA CORPORATION

Inventor： Yuta KATSUTA ,  Nobuo NAKAMURA ,  Shunsuke SATO

IPC: B29C45/00 ,  C08K3/34 ,  C08G63/06

Abstract: A method for producing an injection-molded body from a molding material containing a poly(3-hydroxybutyrate) resin includes: melting the molding material by heating the molding material to a temperature between a melting point peak temperature and a melting point peak end temperature in differential scanning calorimetry analysis of the molding material, wherein the difference between the melting point peak temperature and the melting point peak end temperature of the molding material is from 10 to 70° C.; injecting the molten molding material into a mold having a temperature of 30 to 80° C.; and cooling the molding material in the mold to crystallize and solidify the molding material.

公开(公告)号：US20210340372A1

公开(公告)日：2021-11-04

申请号：US17279940

申请日：2019-09-13

Applicant: KANEKA CORPORATION ,  TOKYO UNIVERSITY OF AGRICULTURE

Inventor： Shunsuke SATO ,  Tomoaki HASHIGUCHI ,  Seiichi TAGUCHI

IPC: C08L67/04 ,  C08J5/18

Abstract: Provided is a resin composition containing polylactic acid and a polyester copolymer of lactic acid and another hydroxycarboxylic acid. The other hydroxycarboxylic acid may be 3-hydroxybutanoic acid, 3-hydroxypentanoic acid, 3-hydroxyhexanoic acid, 3-hydroxyheptanoic acid, 3-hydroxyoctanoic acid, 3-hydroxynonanoic acid, 3-hydroxydecanoic acid 3-hydroxydodecanoic acid, 3-hydroxytetradecanoic acid, 3-hydroxypentadecanoic acid, or 3-hydroxyhexadecanoic acid.

公开(公告)号：US20190082719A1

公开(公告)日：2019-03-21

申请号：US16085287

申请日：2017-03-07

Applicant: KANEKA CORPORATION

Inventor： Shunsuke SATO ,  Tetsuo OKURA

IPC: A23K20/105 ,  A23K10/30 ,  A23K50/80

Abstract: Disclosed herein is a feed composition that promotes the growth of zooplankton, enhances the survival rate of zooplankton, is safe, and has little adverse effect on the environment. The feed composition for zooplankton contains PHA.

公开(公告)号：US20220267547A1

公开(公告)日：2022-08-25

申请号：US17627356

申请日：2020-07-10

Applicant: KANEKA CORPORATION

Inventor： Asuka FUKUTOME ,  Tetsuo OKURA ,  Shunsuke SATO

IPC: B29C71/02 ,  C08G63/06 ,  B29B11/10

Abstract: A method for producing a poly(3-hydroxybutyrate) resin-containing composition for melt processing includes: heating a material containing a poly(3-hydroxybutyrate) resin to a temperature equal to or higher than a melting point peak temperature in differential scanning calorimetry analysis of the poly(3-hydroxybutyrate) resin and equal to or lower than a melting point peak end temperature in the differential scanning calorimetry analysis of the poly(3-hydroxybutyrate) resin, wherein the difference between the melting point peak temperature and the melting point peak end temperature of the poly(3-hydroxybutyrate) resin is 10° C. or more; and extruding the heated material to obtain a composition for melt processing that has a new crystallization peak at a temperature higher than the melting point peak temperature.

公开(公告)号：US20210388324A1

公开(公告)日：2021-12-16

申请号：US17412340

申请日：2021-08-26

Applicant: KANEKA CORPORATION

Inventor： Hisashi ARIKAWA ,  Shunsuke SATO

IPC: C12N9/10 ,  C12P7/62

Abstract: Provided is a transformed microorganism that has a polyhydroxyalkanoate synthase gene and in which expression of a minD gene is enhanced. Also provided is a transformed microorganism that has a polyhydroxyalkanoate synthase gene and in which expression of a minC gene and a minD gene is enhanced. In this transformed microorganism, expression of a minE gene may be enhanced or reduced. Also provided is a method of producing a PHA, the method including the step of culturing any of the transformed microorganisms in the presence of a carbon source.

公开(公告)号：US20240191266A1

公开(公告)日：2024-06-13

申请号：US17995827

申请日：2021-04-08

Applicant: KANEKA CORPORATION

Inventor： Hisashi ARIKAWA ,  Shunsuke SATO ,  Sho FURUTATE

IPC: C12P7/625 ,  C12N1/20 ,  C12N9/10 ,  C12N9/88 ,  C12N15/74

CPC classification number: C12P7/625 ,  C12N1/20 ,  C12N9/1029 ,  C12N9/88 ,  C12N15/74 ,  C12Y402/01017

Abstract: Provided is a method of producing a polyhydroxyalkanoate copolymer mixture. The method includes the step of culturing a microorganism that produces the polyhydroxyalkanoate copolymer mixture. The mixture contains: a fraction (I) containing a polyhydroxyalkanoate copolymer having a 3-hydroxybutyrate structural unit and a 3-hydroxyhexanoate structural unit, the fraction (I) having an average 3-hydroxyhexanoate unit ratio of 20 mol % or more; and a fraction (II) containing a polyhydroxyalkanoate having a 3-hydroxybutyrate structural unit, the fraction (II) having an average 3-hydroxyhexanoate unit ratio of 0 to 15 mol %. The polyhydroxyalkanoate copolymer mixture has an average 3-hydroxyhexanoate unit ratio of 22 mol % or less.

公开(公告)号：US20200087687A1

公开(公告)日：2020-03-19

申请号：US16616276

申请日：2018-05-23

Applicant: KANEKA CORPORATION

Inventor： Rina AOKI ,  Shunsuke SATO

IPC: C12P7/62 ,  C12N9/10 ,  C12N15/74

Abstract: Provided are a PHA-producing microorganism producing a higher molecular weight PHA and a PHA production method using the PHA-producing microorganism. A PHA-producing microorganism including a gene encoding a PHA synthetase derived from genus Aeromonas, in which at least a portion of a PHA degrading enzyme gene is altered by substitution, deletion, insertion, and/or addition to reduce or eliminate activity of a PHA degrading enzyme encoded by the gene, and further a glycerol kinase activity is enhanced.

公开(公告)号：US20170009221A1

公开(公告)日：2017-01-12

申请号：US15115394

申请日：2015-01-30

Applicant: KANEKA CORPORATION

Inventor： Hisashi ARIKAWA ,  Shunsuke SATO ,  Keiji MATSUMOTO

IPC: C12N9/88 ,  C12P7/62 ,  C12N15/74

CPC classification number: C12N9/88 ,  C12N15/74 ,  C12P7/625 ,  C12Y402/01017 ,  C12Y402/01074

Abstract: This invention relates to a microorganism that produces a polyhydroxyalkanoate (PHA) copolymer with a regulated monomer composition ratio and comprises a (R)-specific enoyl-CoA hydratase gene in the genome DNA, wherein a nucleotide sequence upstream of the (R)-specific enoyl-CoA hydratase gene comprises a modification consisting of a substitution(s), a deletion(s), an insertion(s), and/or an addition(s) of one or a plurality of nucleotides so that the expression of the (R)-specific enoyl-CoA hydratase gene is regulated, and to a method for producing a PHA copolymer using the microorganism.

Abstract translation: 本发明涉及产生具有调节的单体组成比的聚羟基链烷酸酯（PHA）共聚物的微生物，并且在基因组DNA中包含（R） - 特异性烯酰辅酶A水合酶基因，其中（R） - 特异性 烯酰辅酶A水合酶基因包含由一个或多个核苷酸的取代，缺失，插入和/或添加组成的修饰物，使得（ R） - 特异性烯酰辅酶A水合酶基因，以及使用该微生物生产PHA共聚物的方法。