公开(公告)号：US20150275187A1

公开(公告)日：2015-10-01

申请号：US14338770

申请日：2014-07-23

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Han Min WOO ,  Hong-Sil PARK ,  Gyeong Taek Gong ,  Youngsoon UM ,  Yunje KIM ,  Dong Jin SUH

IPC: C12N9/02 ,  C12Q1/68 ,  C12P13/00

CPC classification number: C12Q1/6876 ,  C12N9/0006 ,  C12P13/04 ,  C12Q1/689 ,  C12Q2600/142 ,  C12Y101/03021 ,  C12Y103/01032

Abstract: The furfural-resistant strain containing the furfural-resistant gene according to the present disclosure may be effectively grown in a furfural-containing medium. Accordingly, the problem that microorganism fermentation was difficult because toxic by-products such as furfural are contained in a hydrolysate derived from inedible lignocellulosic biomass may be solved. Further, according to the method for producing a strain of the present disclosure, the resistant gene may be selected from relatively small number of target genes. Thus, time, cost and the like for developing the resistant strain may be saved. Further, this method for identifying genes may be broadly applied to methods for identifying various unknown functional genes in addition to the furfural-resistant gene.

Abstract translation: 含有根据本公开的糠醛抗性基因的糠醛抗性菌株可以有效地在含糠醛的培养基中生长。 因此，可以解决微生物发酵困难的问题，因为衍生自不可食用的木质纤维素生物质的水解产物中含有糠醛等有毒副产物。 此外，根据本公开的菌株的制造方法，抗性基因可以选自相对少量的靶基因。 因此，可以节省开发耐药菌株的时间，成本等。 此外，用于鉴定基因的方法可广泛地应用于除了糠醛抗性基因之外鉴定各种未知功能基因的方法。