公开(公告)号：US10155964B2

公开(公告)日：2018-12-18

申请号：US15357815

申请日：2016-11-21

Applicant: Korea Institute of Science and Technology

Inventor： Han Min Woo ,  Sun-young Choi ,  Youngsoon Um ,  Gyeong Taek Gong ,  Sun Mi Lee ,  Yunje Kim

IPC: C12N9/04 ,  C12N9/10 ,  C12N9/90 ,  C12P5/00 ,  C12N15/52

Abstract: The present specification discloses a transformed Synechococcus elongatus strain which may directly produce squalene from carbon dioxide, and a method for producing squalene and a method for removing carbon dioxide, using the same. In an aspect, the strain may produce squalene using carbon dioxide as a carbon source. The Synechococcus elongatus strain is economically efficient because a high-value added squalene is produced using light and carbon dioxide present in the atmosphere as a carbon source, and the method for producing squalene is eco-friendly because the strain may be utilized to remove or reduce carbon dioxide in the atmosphere by using microorganisms. The strain of the present disclosure may produce only squalene, which is a desired target material with high purity, and has an advantage in that squalene may be continuously mass-produced.

公开(公告)号：US20150275187A1

公开(公告)日：2015-10-01

申请号：US14338770

申请日：2014-07-23

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Han Min WOO ,  Hong-Sil PARK ,  Gyeong Taek Gong ,  Youngsoon UM ,  Yunje KIM ,  Dong Jin SUH

IPC: C12N9/02 ,  C12Q1/68 ,  C12P13/00

CPC classification number: C12Q1/6876 ,  C12N9/0006 ,  C12P13/04 ,  C12Q1/689 ,  C12Q2600/142 ,  C12Y101/03021 ,  C12Y103/01032

Abstract: The furfural-resistant strain containing the furfural-resistant gene according to the present disclosure may be effectively grown in a furfural-containing medium. Accordingly, the problem that microorganism fermentation was difficult because toxic by-products such as furfural are contained in a hydrolysate derived from inedible lignocellulosic biomass may be solved. Further, according to the method for producing a strain of the present disclosure, the resistant gene may be selected from relatively small number of target genes. Thus, time, cost and the like for developing the resistant strain may be saved. Further, this method for identifying genes may be broadly applied to methods for identifying various unknown functional genes in addition to the furfural-resistant gene.

Abstract translation: 含有根据本公开的糠醛抗性基因的糠醛抗性菌株可以有效地在含糠醛的培养基中生长。 因此，可以解决微生物发酵困难的问题，因为衍生自不可食用的木质纤维素生物质的水解产物中含有糠醛等有毒副产物。 此外，根据本公开的菌株的制造方法，抗性基因可以选自相对少量的靶基因。 因此，可以节省开发耐药菌株的时间，成本等。 此外，用于鉴定基因的方法可广泛地应用于除了糠醛抗性基因之外鉴定各种未知功能基因的方法。

公开(公告)号：US09719097B2

公开(公告)日：2017-08-01

申请号：US14860373

申请日：2015-09-21

Applicant: Korea Institute of Science and Technology

Inventor： Han Min Woo ,  Jun Won Chwa ,  Youngsoon Um ,  Gyeong Taek Gong ,  Yunje Kim

IPC: C07H21/02 ,  C07H21/04 ,  A61K48/00 ,  C12N15/74

CPC classification number: C12N15/74

Abstract: The present disclosure discloses a vector that can be used for both cyanobacteria and E. coli, which contains, sequentially, a pUC replication origin as a replication origin; a spectinomycin-resistant gene as a selection marker; and a promoter selected from a group consisting of a trc promoter, a tetA promoter or a modified tetA promoter, a BAD promoter and a cbbL promoter. An industrially useful substance may be produced effectively using a host cell transformed with the vector. Also, the vector may be used to insert a variety of target genes through simple combination and, as a result, various vectors can be prepared effectively.

公开(公告)号：US10036046B2

公开(公告)日：2018-07-31

申请号：US15299188

申请日：2016-10-20

Applicant: Korea Institute of Science and Technology

Inventor： Han Min Woo ,  Hyun Jeong Lee ,  Youngsoon Um ,  Gyeong Taek Gong ,  Sun Mi Lee ,  Yunje Kim

IPC: C12N1/20 ,  C12N15/70 ,  C12P7/64 ,  C07H21/04 ,  C12N9/04 ,  C12N9/10 ,  C12N9/16 ,  C12N9/88 ,  C12N15/52

CPC classification number: C12P7/649 ,  C12N9/0006 ,  C12N9/1029 ,  C12N9/16 ,  C12N9/88 ,  C12N15/52 ,  C12P7/6463 ,  C12Y101/01001 ,  C12Y401/01001 ,  Y02E50/13

Abstract: The present disclosure discloses a transformed Synechococcus elongatus strain capable of producing biodiesel directly from carbon dioxide and a method for producing biodiesel and a method for removing carbon dioxide using the same. In an aspect, the transformed Synechococcus elongatus strain of the present disclosure can produce biodiesel in large scale using carbon dioxide as a carbon source. The Synechococcus elongatus strain is environment-friendly because it can be used to remove or reduce carbon dioxide in the atmosphere. The strain of the present disclosure is advantageous in that it can produce biodiesel in large scale because it grows faster and exhibits excellent carbon dioxide fixation capability as compared to other photosynthetic microorganisms.

公开(公告)号：US09441230B2

公开(公告)日：2016-09-13

申请号：US14637355

申请日：2015-03-03

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Han Min Woo ,  Youngsoon Um ,  Yunje Kim ,  Gyeong Taek Gong ,  Jung Seok Lee

IPC: C12N15/77 ,  C12N15/72

CPC classification number: C12N15/77 ,  C12N15/72

Abstract: Disclosed is a shuttle vector that can be used for Corynebacterium and E. coli, containing: a repressor selected from a group consisting of a lacI repressor and a tetR repressor; a promoter selected from a group consisting of a trc promoter, a tetA promoter and a LacUV5 promoter; a replication origin pBL1 derived from Corynebacterium glutamicum; and a replication origin ColE1 of E. coli. A host cell transformed with the shuttle vector can effectively produce industrially useful substances. Also, the shuttle vector may be used to easily insert various combinations of target genes and, as a result, a variety of vectors can be prepared effectively.

Abstract translation: 公开了可用于棒状杆菌和大肠杆菌的穿梭载体，其包含：选自lacI阻遏物和tetR阻遏物的阻遏物; 选自由trc启动子，tetA启动子和LacUV5启动子组成的组的启动子; 衍生自谷氨酸棒杆菌的复制起点pBL1; 和大肠杆菌的复制起点ColE1。 用穿梭载体转化的宿主细胞可以有效地产生工业上有用的物质。 此外，穿梭载体可以用于容易地插入靶基因的各种组合，结果可以有效地制备各种载体。