公开(公告)号：US20160130620A1

公开(公告)日：2016-05-12

申请号：US14934710

申请日：2015-11-06

Applicant: Korea Institute of Science and Technology

Inventor： Yunje KIM ,  Youngsoon UM ,  Han Min WOO ,  Gyeongtaek GONG ,  Kyoungseon MIN

IPC: C12P19/14 ,  C12P19/02

CPC classification number: C12P19/14 ,  C12N9/0061 ,  C12N9/0065 ,  C12P19/02 ,  Y02E50/343

Abstract: The present disclosure relates to a method for simultaneously degrading lignin and cellulose and for boosting effect on the cellulase activity using a specific catalyst. Since the present disclosure allows for the preparation of sugars by degrading not only lignin but also cellulose and hemicellulose using the enzymes which were previously known only as lignin-degrading biocatalysts, it provides the advantage that the preparation of a hydrolysate as a source material for the production of biofuels or biochemicals from lignocellulosic biomass can be simplified and facilitated. As a result, the present disclosure can reduce enzyme cost and can provide improved production efficiency by simplifying the biofuel production process.

Abstract translation: 本公开内容涉及同时降解木质素和纤维素并且使用特定催化剂对纤维素酶活性的促进作用的方法。 由于本公开允许通过使用先前已知的仅作为木质素降解生物催化剂的酶降解木质素而且降解纤维素和半纤维素来制备糖，因此其具有以下优点：制备水解产物作为 可以简化和促进从木质纤维素生物质生产生物燃料或生物化学品。 结果，本公开可以降低酶成本，并且可以通过简化生物燃料生产过程来提供提高的生产效率。

公开(公告)号：US20170283832A1

公开(公告)日：2017-10-05

申请号：US15357815

申请日：2016-11-21

Applicant: Korea Institute of Science and Technology

Inventor： Han Min WOO ,  Sun-young CHOI ,  Youngsoon UM ,  Gyeong Taek GONG ,  Sun Mi LEE ,  Yunje KIM

IPC: C12P5/00 ,  C12N9/90 ,  C12N9/10 ,  C12N15/52 ,  C12N9/04

CPC classification number: C12P5/007 ,  C12N9/0006 ,  C12N9/1022 ,  C12N9/1085 ,  C12N9/90 ,  C12N15/52 ,  C12Y101/01267 ,  C12Y202/01007 ,  C12Y205/0101 ,  C12Y205/01021 ,  C12Y503/03002

Abstract: The present specification discloses a transformed Synechococcus elongatus strain which may directly produce squalene from carbon dioxide, and a method for producing squalene and a method for removing carbon dioxide, using the same. In an aspect, the strain may produce squalene using carbon dioxide as a carbon source. The Synechococcus elongatus strain is economically efficient because a high-value added squalene is produced using light and carbon dioxide present in the atmosphere as a carbon source, and the method for producing squalene is eco-friendly because the strain may be utilized to remove or reduce carbon dioxide in the atmosphere by using microorganisms. The strain of the present disclosure may produce only squalene, which is a desired target material with high purity, and has an advantage in that squalene may be continuously mass-produced.

公开(公告)号：US20170114375A1

公开(公告)日：2017-04-27

申请号：US15299188

申请日：2016-10-20

Applicant: Korea Institute of Science and Technology

Inventor： Han Min WOO ,  Hyun Jeong LEE ,  Youngsoon UM ,  Gyeong Taek GONG ,  Sun Mi LEE ,  Yunje KIM

IPC: C12P7/64 ,  C12N1/20

CPC classification number: C12P7/649 ,  C12N9/0006 ,  C12N9/1029 ,  C12N9/16 ,  C12N9/88 ,  C12N15/52 ,  C12Y101/01001 ,  C12Y401/01001

Abstract: The present disclosure discloses a transformed Synechococcus elongatus strain capable of producing biodiesel directly from carbon dioxide and a method for producing biodiesel and a method for removing carbon dioxide using the same. In an aspect, the transformed Synechococcus elongatus strain of the present disclosure can produce biodiesel in large scale using carbon dioxide as a carbon source. The Synechococcus elongatus strain is environment-friendly because it can be used to remove or reduce carbon dioxide in the atmosphere. The strain of the present disclosure is advantageous in that it can produce biodiesel in large scale because it grows faster and exhibits excellent carbon dioxide fixation capability as compared to other photosynthetic microorganisms.

公开(公告)号：US20170009240A1

公开(公告)日：2017-01-12

申请号：US14950425

申请日：2015-11-24

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Han Min WOO ,  Eun Mi KIM ,  Youngsoon UM ,  Gyeongtaek GONG ,  Sun Mi LEE ,  Yunje KIM

IPC: C12N15/52 ,  C12N15/71 ,  C12N9/90 ,  C12N9/88 ,  C12N9/04 ,  C12N9/12 ,  C12N15/72 ,  C12N9/10

CPC classification number: C12N15/52 ,  C12N9/0006 ,  C12N9/1029 ,  C12N9/1085 ,  C12N9/1205 ,  C12N9/1229 ,  C12N9/88 ,  C12N9/90 ,  C12N15/70 ,  C12N15/71 ,  C12N15/72 ,  C12Y101/01034 ,  C12Y203/01026 ,  C12Y205/01 ,  C12Y207/01036 ,  C12Y207/04002 ,  C12Y401/01033 ,  C12Y401/03 ,  C12Y503/03002

Abstract: Disclosed herein are an expression vector capable of expressing myrcene, an Escherichia coli strain transformed with the vector and having improved capability of producing myrcene and a method for producing myrcene and a method for recycling glycerol using the same. In an aspect, the transformed Escherichia coli strain of the present disclosure can produce myrcene with high purity on a large scale using glycerol or glucose as a carbon source. Also, the Escherichia coli strain of the present disclosure is economical and environment-friendly because it can produce high value-added myrcene using waste glycerol as a carbon source. In addition, the strongly volatile myrcene can be produced and isolated at the same time.

Abstract translation: 本文公开了能够表达月桂烯的表达载体，用载体转化的大肠杆菌菌株，并且具有改进的生产月桂烯的能力和用于制备月桂烯的方法和使用该方法回收甘油的方法。 在一方面，本公开的转化的大肠杆菌菌株可以使用甘油或葡萄糖作为碳源大量生产高纯度的月桂烯。 此外，本公开的大肠杆菌菌株是经济和环境友好的，因为它可以使用废甘油作为碳源产生高附加值的月桂烯。 此外，强烈挥发性月桂烯可以同时生产和分离。

公开(公告)号：US20160168580A1

公开(公告)日：2016-06-16

申请号：US14860373

申请日：2015-09-21

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Han Min WOO ,  Jun Won CHWA ,  Youngsoon UM ,  Gyeong Taek GONG ,  Yunje KIM

IPC: C12N15/74

CPC classification number: C12N15/74

Abstract: The present disclosure discloses a vector that can be used for both cyanobacteria and E. coli, which contains, sequentially, a pUC replication origin as a replication origin; a spectinomycin-resistant gene as a selection marker; and a promoter selected from a group consisting of a trc promoter, a tetA promoter or a modified tetA promoter, a BAD promoter and a cbbL promoter. An industrially useful substance may be produced effectively using a host cell transformed with the vector. Also, the vector may be used to insert a variety of target genes through simple combination and, as a result, various vectors can be prepared effectively.

Abstract translation: 本公开公开了可以用于蓝细菌和大肠杆菌的载体，其包含顺序地作为复制起点的pUC复制起点; 壮观霉素抗性基因作为选择标记; 和选自trc启动子，tetA启动子或修饰的tetA启动子，BAD启动子和cbbL启动子的启动子。 可以使用用载体转化的宿主细胞有效地产生工业上有用的物质。 此外，载体可以用于通过简单组合插入各种靶基因，结果可以有效地制备各种载体。

公开(公告)号：US20160152942A1

公开(公告)日：2016-06-02

申请号：US14679622

申请日：2015-04-06

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Han Min WOO ,  Youngsoon UM ,  Gyeong Taek GONG ,  Dong Jin SUH ,  Yunje KIM

IPC: C12N1/20

CPC classification number: C12N1/20 ,  C12R1/01

Abstract: A method for isolating a highly furfural-resistant strain is disclosed. The method includes (A) mixing soil with an isotonic solution and collecting the soil supernatant from the mixture, (B) diluting the soil supernatant and spreading the dilution on a medium supplemented with carboxymethyl cellulose as a nutrient source, and (C) growing a desired strain by culture in the medium spread with the diluted soil supernatant and isolating the strain. Also disclosed is a strain isolated by the method. The strain is Enterobacter cloacae strain GGT036 [Accession No. KCTC 12672BP].

Abstract translation: 公开了一种分离高糠醛抗性菌株的方法。 该方法包括（A）将土壤与等渗溶液混合并从混合物中收集土壤上清液，（B）稀释土壤上清液并将稀释液铺展在补充有羧甲基纤维素作为营养源的培养基上，（C） 通过在稀释的土壤上清液中培养的培养物中所需的菌株并分离菌株。 还公开了通过该方法分离的菌株。 菌株为阴沟肠杆菌菌株GGT036 [保藏号KCTC 12672BP]。

公开(公告)号：US20160024535A1

公开(公告)日：2016-01-28

申请号：US14874040

申请日：2015-10-02

Applicant: Korea Institute of Science and Technology

Inventor： Youngsoon UM ,  Yunje KIM ,  Dongjin SUH ,  Kyung Min LEE ,  Jeong Myeong HA

IPC: C12P7/52 ,  C12P7/16

CPC classification number: C12P7/52 ,  C12P7/10 ,  C12P7/16 ,  C12P7/64 ,  C12P19/02 ,  C12P19/14 ,  C12P2201/00 ,  C12P2203/00 ,  Y02E50/10 ,  Y02E50/16 ,  Y02E50/343

Abstract: Provided is a method for producing a saccharified solution of wood-based biomass having toxicity reduced or removed, comprising the steps of: preparing a saccharified solution in which wood-based biomass has been pretreated by hydrolysis; and reducing toxicity in the saccharified solution using an electrochemical detoxification method. The method is capable of efficiently removing the toxicity of compounds which are produced during the pretreatment process and inhibit growth of microorganisms and fermentation. In addition, the method is capable of increasing the efficiency of production by minimizing sugar loss and additional costs during the toxicity removal process.

Abstract translation: 本发明提供一种生产具有毒性降低或去除的木质生物质的糖化溶液的方法，包括以下步骤：制备通过水解预处理木质生物质的糖化溶液; 并使用电化学解毒方法降低糖化溶液中的毒性。 该方法能够有效地去除在预处理过程中产生的化合物的毒性并抑制微生物的生长和发酵。 此外，该方法能够通过最小化糖损失和毒性去除过程中的额外成本来提高生产效率。

公开(公告)号：US20160298146A1

公开(公告)日：2016-10-13

申请号：US15092829

申请日：2016-04-07

Applicant: Korea Institute of Science and Technology

Inventor： Youngsoon UM ,  Yunje KIM ,  Kyung Min LEE ,  Han Min WOO ,  Gyeongtaek GONG ,  Ki Yeon KIM

IPC: C12P7/52

CPC classification number: C12P7/52 ,  C12P19/02 ,  C12P19/12 ,  C12P2203/00 ,  Y02E50/17 ,  Y02E50/343

Abstract: The present disclosure relates to a hydrolysate of a mixture of lignocellulosic biomass and seaweed biomass. By mixing seaweed biomass with lignocellulosic biomass and then preparing a hydrolysate, lignocellulosic biomass-derived acetic acid is consumed together with seaweed biomass-derived mannitol. As a result, high sugar productivity can be maintained while reducing fermentation inhibitors. Because the present disclosure can solve the problem of lignocellulosic biomass of decreased fermentation efficiency due to lignocellulose-derived fermentation inhibitors and the problem of seaweed biomass of very low productivity in spite of long fermentation time, the hydrolysate according to the present disclosure may be used to produce biofuels and biochemicals economically.

Abstract translation: 本公开涉及木质纤维素生物质和海藻生物质的混合物的水解产物。 通过将海藻生物质与木质纤维素生物质混合，然后制备水解产物，木质纤维素生物质衍生的乙酸与海藻生物质衍生的甘露醇一起消耗。 因此，可以在减少发酵抑制剂的同时保持高的糖生产率。 因为本公开可以解决由于木素纤维素衍生的发酵抑制剂引起的发酵效率降低的木质纤维素生物质的问题和尽管发酵时间长的生产效率非常低的海藻生物质的问题，但根据本公开的水解产物可用于 经济地生产生物燃料和生物化学品。

公开(公告)号：US20160115491A1

公开(公告)日：2016-04-28

申请号：US14637355

申请日：2015-03-03

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Han Min WOO ,  Youngsoon UM ,  Yunje KIM ,  Gyeong Taek GONG ,  Jung Seok LEE

IPC: C12N15/77 ,  C12N15/72

CPC classification number: C12N15/77 ,  C12N15/72

Abstract: Disclosed is a shuttle vector that can be used for Corynebacterium and E. coli, containing: a repressor selected from a group consisting of a lacI repressor and a tetR repressor; a promoter selected from a group consisting of a trc promoter, a tetA promoter and a LacUV5 promoter; a replication origin pBL1 derived from Corynebacterium glutamicum; and a replication origin ColE1 of E. coli. A host cell transformed with the shuttle vector can effectively produce industrially useful substances. Also, the shuttle vector may be used to easily insert various combinations of target genes and, as a result, a variety of vectors can be prepared effectively.

Abstract translation: 公开了可用于棒状杆菌和大肠杆菌的穿梭载体，其包含：选自lacI阻遏物和tetR阻遏物的阻遏物; 选自由trc启动子，tetA启动子和LacUV5启动子组成的组的启动子; 衍生自谷氨酸棒杆菌的复制起点pBL1; 和大肠杆菌的复制起点ColE1。 用穿梭载体转化的宿主细胞可以有效地产生工业上有用的物质。 此外，穿梭载体可以用于容易地插入靶基因的各种组合，结果可以有效地制备各种载体。

公开(公告)号：US20160002677A1

公开(公告)日：2016-01-07

申请号：US14494930

申请日：2014-09-24

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Han Min WOO ,  Jung Seok LEE ,  Youngsoon UM ,  Gyeong Taek GONG ,  Byoung Koun MIN ,  Jeong-Myeong HA ,  Yunje KIM ,  Sang Jun SIM

IPC: C12P7/46 ,  C12N15/77

CPC classification number: C12P7/46 ,  C12N15/77

Abstract: The present disclosure relates to a strain capable of producing succinate using starch accumulated in microalgae which grow using carbon dioxide as a direct carbon source without converting it to glucose and a method for producing succinate using the same. The present disclosure provides a strain producing succinate from carbon dioxide, selected from a group consisting of Corynebacterium glutamicum BL-1-pBlAmyS (KCTC 12585BP) and Corynebacterium glutamicum BL-1-pSbAmyA (KCTC 12587BP). The present disclosure also provides a method for producing succinate from carbon dioxide, including fermenting starch by inoculating the strain producing succinate from carbon dioxide in a starch-containing medium.

Abstract translation: 本公开涉及使用在二氧化碳作为直接碳源而不将其转化为葡萄糖而生长的微藻中积累的淀粉生产琥珀酸的菌株，以及使用该淀粉生产琥珀酸盐的方法。 本公开提供了从二氧化碳生产琥珀酸盐的菌株，其选自谷氨酸棒杆菌BL-1-pBlAmyS（KCTC 12585BP）和谷氨酸棒杆菌BL-1-pSbAmyA（KCTC 12587BP）。 本公开还提供了一种从二氧化碳生产琥珀酸的方法，包括通过在含淀粉培养基中从二氧化碳接种产生琥珀酸的菌株来发酵淀粉。